Difference between revisions of "Team:Yale/Parts"

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<h2> Part Documentation</h2>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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    <title>Yale iGem 2015: Project Parts</title>
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<div class="highlightBox">
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    <meta name="author" content="Cameron Yick">
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<h4>Note</h4>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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    <link href="http://fonts.googleapis.com/css?family=Noto+Sans" rel="stylesheet" type="text/css">
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    <link href="http://client.cameronyick.us/igem/assets/styles/main.min.css" rel="stylesheet" type="text/css">
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    <nav class="nav-contain">
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<h4>Adding parts to the registry</h4>
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      <ul class="nav">
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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        <li> <a href="https://2015.igem.org/Team:Yale" class="logoMark"> <span>Non</span><span>Model</span></a>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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        </li>
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        <li class="submenu"><a href="https://2015.igem.org/Team:Yale/project">Project</a>
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          <ul>
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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            <li><a href="https://2015.igem.org/Team:Yale/project#overview" alt="Overview">Overview</a></li>
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<p>The information needed to initially create a part on the Registry is:</p>
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            <li><a href="https://2015.igem.org/Team:Yale/methods" alt="Methods">Methods</a></li>
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<ul>
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            <li><a href="https://2015.igem.org/Team:Yale/results" alt="Results">Results</a></li>
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<li>Part Name</li>
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            <li><a href="https://2015.igem.org/Team:Yale/modeling" alt="Modeling">Modeling</a></li>
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<li>Part type</li>
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            <li><a href="https://2015.igem.org/Team:Yale/parts" alt="Parts">Parts</a></li>
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<li>Creator</li>
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          </ul>
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<li>Sequence</li>
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        </li>
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<li>Short Description (60 characters on what the DNA does)</li>
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        <li><a href="https://2015.igem.org/Team:Yale/notebook" alt="Notebook">Notebook</a></li>
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<li>Long Description (Longer description of what the DNA does)</li>
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        <li class="submenu"><a href="collaborations">Collaborations</a></li>
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<li>Design considerations</li>
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        <li class="submenu"><a href="practices">Human Practices</a>
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</ul>
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          <ul>
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            <li><a href="https://2015.igem.org/Team:Yale/practices#video" alt="Documentary">Documentary</a></li>
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<p>
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            <li><a href="https://2015.igem.org/Team:Yale/practices#ssri" alt="SSRI">SSRI</a></li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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            <li><a href="https://2015.igem.org/Team:Yale/practices#lgbtq" alt="LGBTQ Survey">LGBTQ Survey</a></li>
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          </ul>
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        </li>
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        <li class="submenu"><a href="team">Team</a>
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          <ul>
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            <li><a href="https://2015.igem.org/Team:Yale/team#people" alt="People">People</a></li>
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            <li><a href="https://2015.igem.org/Team:Yale/team#acknowledgements" alt="Acknowledgements">Acknowledgements</a></li>
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<h4>Inspiration</h4>
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          </ul>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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        </li>
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        <li class="submenu"><a href="standards">Standards</a>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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          <ul>
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<ul>
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            <li><a href="https://2015.igem.org/Team:Yale/standards#gold" alt="Gold">Gold</a></li>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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            <li><a href="https://2015.igem.org/Team:Yale/standards#silver" alt="Silver">Silver</a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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            <li><a href="https://2015.igem.org/Team:Yale/standards#bronze" alt="Bronze">Bronze</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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          </ul>
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</ul>
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        </li>
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      </ul>
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    </nav>
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    <section class="findout__section">
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<h4>Part Table </h4>
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      <h2 class="page__head">Developing a Framework for the Genetic Manipulation of Non-Model and Environmentally Significant Microbes</h2>
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</html>
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      <div class="row overview__blocks">
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<groupparts>iGEM015 Example</groupparts>
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        <div class="small-12 switch-border">
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          <h2><a href="project#overview" alt="Overview">Overview</a></h2>
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          <h2><a href="methods" alt="Methods">Methods</a></h2>
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          <h2><a href="results" alt="Results">Results</a></h2>
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          <h2><a href="modeling" alt="Modeling">Modeling</a></h2>
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</div>
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          <h2><a href="parts" alt="Parts">Parts</a></h2>
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        </div>
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      </div>
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    </section>
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    <section class="content__section">
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      <h2 id="overview">Parts List
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        <h3>These are our legos.</h3>
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        <div class="page__button"><a href="#partfull" class="custom__button">Full Molecule</a><a href="#part1" class="custom__button">Part One</a><a href="#part2" class="custom__button">Part Two</a>
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        </div>
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      </h2>
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    </section>
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    <section class="content__section">
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      <p> Our collection of submitted biobricks consists of:</p>
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      <ul>
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        <li>Mussel foot protein (MFP) 1-5-1 sequence [combination of Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus li Edulis Foot Protein 1 (Mefp-1)].</li>
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        <li>MFP with superfolder Green Fluorescence Protein (sfGFP).</li>
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        <li>MFP with our anti-microbial peptide, LL-37.</li>
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        <li>Entire construct of our anti-microbial adhesive peptide: 2XStrep_Flagtag--LL-37--Mussel Foot Protein--sfGFP.  </li>
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      </ul>
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    </section>
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    <section class="content__section">
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      <h2 id="partfull">Full Molecule</h2>
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      <h3>BBa_K1396000</h3>
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      <div class="row content__dark part__row">
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        <div class="small-5 columns readable">
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          <h4>Testing Promoters</h4>
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          <p>The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>
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        </div>
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        <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2014/b/b7/Full_Construct.jpg"></div>
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      </div>
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    </section>
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    <section class="content__section">
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      <h2 id="part1">Part 1: LL-37-MFP:</h2>
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      <h3>Based on BBa_K1396000</h3>
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      <div class="row content__dark part__row">
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        <div class="small-5 columns readable">
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          <h4>Feeding Fish</h4>
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          <p>The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.tion. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.</p>
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        </div>
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        <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2014/f/f4/LL37_FP151.jpg">
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        </div>
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      </div>
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    </section>
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    <section class="content__section">
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      <h2 id="part2">Part 2: MFP-sfGFP: </h2>
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      <h3>Based on BBa_K1396002</h3>
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      <div class="row content__dark part__row">
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        <div class="small-5 columns readable">
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          <h4>Harvesting Oysters</h4>
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          <p>The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>
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        </div>
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        <div class="small-7 columns readable"><img src="https://static.igem.org/mediawiki/2014/5/5b/FP151_GFP.jpg" ,data-width="565" data-height="525" class="img"></div>
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      </div>
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    </section>
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    <div class="igem__footer">
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      <div class="foot__contain">
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        <div class="row foot__row"><img src="http://client.cameronyick.us/igem/assets/img/iGEM_Tree_Round_Red.png" class="foot__logo">
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          <div class="small-7 columns foot__text">
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            <h2>Yale iGem 2015</h2>
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            <p>Main Campus:</p>
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            <p>Yale Department of Molecular, Cellular & Developmental Biology</p>
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            <p>Attn: Farren Isaacs/iGEM</p>
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            <p>219 Prospect St</p>
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            <p>PO Box 208103</p>
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            <p>New Haven, CT 06520</p>
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            <p>Tel. +1(203) 432-3783</p>
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            <p>E-mail: igem.yale@gmail.com</p>
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          </div><img src="http://client.cameronyick.us/igem/assets/img/iGEM_Logo.png" class="igem__logo">
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        </div>
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        <div class="row">
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          <p class="text-center">&copy; Yale iGEM 2015</p>
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Latest revision as of 01:31, 18 September 2015


<!DOCTYPE html> Yale iGem 2015: Project Parts

Developing a Framework for the Genetic Manipulation of Non-Model and Environmentally Significant Microbes

Overview

Methods

Results

Modeling

Parts

Parts List

These are our legos.

Full MoleculePart OnePart Two

Our collection of submitted biobricks consists of:

  • Mussel foot protein (MFP) 1-5-1 sequence [combination of Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus li Edulis Foot Protein 1 (Mefp-1)].
  • MFP with superfolder Green Fluorescence Protein (sfGFP).
  • MFP with our anti-microbial peptide, LL-37.
  • Entire construct of our anti-microbial adhesive peptide: 2XStrep_Flagtag--LL-37--Mussel Foot Protein--sfGFP.

Full Molecule

BBa_K1396000

Testing Promoters

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.

Part 1: LL-37-MFP:

Based on BBa_K1396000

Feeding Fish

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.tion. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.

Part 2: MFP-sfGFP:

Based on BBa_K1396002

Harvesting Oysters

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.

Yale iGem 2015

Main Campus:

Yale Department of Molecular, Cellular & Developmental Biology

Attn: Farren Isaacs/iGEM

219 Prospect St

PO Box 208103

New Haven, CT 06520

Tel. +1(203) 432-3783

E-mail: igem.yale@gmail.com

© Yale iGEM 2015