Difference between revisions of "Team:Gifu/result-page"
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<p><b>Semi-quantitative PCR </b></p> | <p><b>Semi-quantitative PCR </b></p> | ||
− | <p> The result of Semi-quantitative PCR was shown below [ raw data ]. And, we made the graphs by using the raw data. | + | <p> The result of Semi-quantitative PCR was shown below [ raw data ]. And, we made the graphs by using the raw data. |
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<img src="https://static.igem.org/mediawiki/2015/1/1b/Gifu-kekka-kekka.png" width="450" height="96" vspace="10" hspace="30" align="right"></img> | <img src="https://static.igem.org/mediawiki/2015/1/1b/Gifu-kekka-kekka.png" width="450" height="96" vspace="10" hspace="30" align="right"></img> | ||
− | <p> Ct is a value that the more the gene template in PCR increases, the more Ct value decreases. If there is a difference between “C” and “D”, there is a difference in the quantity of template geneThus, the difference between “C” and “D” is small; the cyclization may be efficiency.As a result, Ct in "normal" was 2.31, but “outside”, “inside ①”, “inside ②” were 1.28, 2.02, 1.89 collectively. From these things, it can be said that the efficiency of cyclization rose with all devices which we designed in this time. Especially, in the case of “outside”, there was a difference with Ct more than 1 point than “normal”. When we think that a quantity of the gene doubles for 1 cycle in PCR simply, it can be said that the cyclic efficiency of “outside” was twice as high as that of “normal”. <br> | + | <p> We compared each Ct value at the same fluorescence intensity. These value is summarized right. Ct is a value that the more the gene template in PCR increases, the more Ct value decreases. If there is a difference between “C” and “D”, there is a difference in the quantity of template geneThus, the difference between “C” and “D” is small; the cyclization may be efficiency.As a result, Ct in "normal" was 2.31, but “outside”, “inside ①”, “inside ②” were 1.28, 2.02, 1.89 collectively. From these things, it can be said that the efficiency of cyclization rose with all devices which we designed in this time. Especially, in the case of “outside”, there was a difference with Ct more than 1 point than “normal”. When we think that a quantity of the gene doubles for 1 cycle in PCR simply, it can be said that the cyclic efficiency of “outside” was twice as high as that of “normal”. <br> |
After all, it is thought that it fitted this splice site because it is a complementarity chain derived from the creature. | After all, it is thought that it fitted this splice site because it is a complementarity chain derived from the creature. | ||
</p> | </p> |
Revision as of 03:45, 18 September 2015