Difference between revisions of "Team:BIT/Description"

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{{BIT}}
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    <meta name="author" content="Zhangyan">
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    <meta name="keyword" content="igem, BIT,  iGem_BIT, China, BIOLOGICAL">
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    <link rel="shortcut icon" href="img/favicon.png">
  
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<h2> Project Description </h2>
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    <title>iGem_BIT</title>
  
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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    <!-- Bootstrap core CSS -->
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<br />
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    <link href="https://2015.igem.org/Team:BIT/css/bootstrap-min?action=raw&amp;ctype=text/css" rel="stylesheet">
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<h5>What should this page contain?</h5>
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    <link rel="stylesheet" href="https://2015.igem.org/Team:BIT/assets/css/rs-style?action=raw&amp;ctype=text/css" media="screen">
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<ul>
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    <link rel="stylesheet" href="https://2015.igem.org/Team:BIT/assets/css/settings?action=raw&amp;ctype=text/css" media="screen">
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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</ul>
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<h4>Advice on writing your Project Description</h4>
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<style type="text/css">
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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</p>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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</p>
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  <body>
  
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    <div id="content">
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<h4>References</h4>
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<div id="bodyContent">
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
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<h4>Inspiration</h4>
 
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<p>See how other teams have described and presented their projects: </p>
 
  
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<ul>
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    <!--header start-->
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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    <header class="header-frontend">
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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        <div class="navbar navbar-default navbar-static-top">
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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                    <a class="navbar-brand" href="index.html">Disease <span>Alarm</span></a>
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                        <li><a href="https://2015.igem.org/Team:BIT">Home</a></li>
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                        <li  class="active" class="dropdown ">
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                            <a href="#" class="dropdown-toggle " data-toggle="dropdown" data-hover="dropdown" data-delay="0" data-close-others="false">Project <b class=" icon-angle-down"></b></a>
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                            <ul class="dropdown-menu">
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                    <li class="active"><a href="https://2015.igem.org/Team:BIT/Biology">Biology Part</a></li>
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                    <li><a href="https://2015.igem.org/Team:BIT/Hardware">Hardware Part</a></li>                           </ul>
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                        </li>   
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                        <li><a href="https://2015.igem.org/Team:BIT/Attributions">Attributions</a></li>                          
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                        <li><a href="https://2015.igem.org/Team:BIT/Practices">Practices</a></li>
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                                <li><a href="https://2015.igem.org/Team:BIT/Collaborations">Collaboration</a></li>
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                                <li><a href="https://2015.igem.org/Team:BIT/Description">Description</a></li>
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                        <li><a href="https://2015.igem.org/Team:BIT/Team">Team</a></li>
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                        <li><a href="https://2015.igem.org/Team:BIT/Gallery">Gallery</a></li>
  
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                    <h1>Description</h1>
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                        <h1>BIOLOGY</h1>
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<H3>Improvement</H3>
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                            <p><span class="dropcap">C</span>ompared to last year’s project, the project this year brings in a new amplifying system in signal amplifying for microbe, which significantly improves the efficiency of signal amplifying. For the feature of project, we build a cascade amplifier in microbe by imitating cascade amplifier in circuitry, based on amplifying system of last year.
 +
<br>
 +
<br>
 +
Last year: In the plasmid of amplification system, two activating transcription factors are in constant expression and remain in a certain concentration. With a certain level of autoinducer PAO1, PAO1 integrates with lasR, the complex of which are bond to the target site of las promoter, then activating it and control the expression of the downstream reporter gene GFP in accord with the concentration of the amount of combination.
 +
<br><br>
 +
This year: Inside the upriver bacteria, certain enzyme will digest the oligopeptides that is from glycopeptide so that the arabinose will be released and induce the engineering bacteria expressing LasI protein. The protein can catalyze matrix producing a large number of small molecules PAI1. When PAI1 free diffuse into the downstream bacteria, it will combine with the constitutive expressed LasR protein and form a transcription factor complex. The combination between complex and the pLas promoter accelerate the process of transcription and translation to generate the Rhl1 enzyme, which can catalyze producing the autoinducer PAI2 from system RhIIR. The complex of PAI2 and RhIR protein that is mediated-expressed by double promoter in bacteria can act on pRhl promoter and induce the engineering bacteria to express GFP.
 +
<br><br>
 +
 +
<H3>Innovation</H3>
 +
<span class="dropcap">W</span>e use aptamers as intermedia innovatively, which forms a competitive inhibition system, so as to realize the goal this year that detecting biochemical macromolecules by microbe.
 +
<br><br>
 +
This year: Outside bacteria, we combine the artificial oligopeptides with the aptamers. As the macromolecules get into the measurement system, they will take places of glycopeptide and leave them as dissociated molecules. Finally, these free glycopeptide will enter the transformed engineering bacteria.
 +
<br><br>
 +
 +
<h1>HARDWARE</h1>
 +
<H3>Improvement</H3>
 +
                           
 +
<p><span class="dropcap">F</span>or fluorescence detection device, the improvement are as following. First of all, we use blue laser pen instead of blue LED light, for its light concentrates better and the energy is more powerful, which remarkably improves the efficiency of fluorescent protein stimulation. Secondly, given that fluorescent chromaticity is single, we use sensor OPT101 instead of sensor TCS32XX to improve accuracy, which is sensitive to grayscale rather than chromaticity. Finally, we use UART screen instead of LED panel, which sharply improves the user interaction interface and is more interactive.
 +
<br>
 +
<br>
 +
 +
<H3>Innovation</H3>
 +
<span class="dropcap">I</span>n order to make microbe express fluorescent protein rapidly, we innovatively build a bacterial culture device, which refers to thermostatic shaker in laboratory. The details is shown in “Hardware-Design”.
 +
<br><br>
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                      </div>
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                    <h1>contact info</h1>
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                    <address>
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                        <p>Address: Beijing Institute of Technology, No. 5 South Zhong Guan Cun Street,
 +
Haidian Beijing 100081, P. R. China </p>
 +
 +
                        <p>Twitter : @igem_BIT</p>
 +
                        <p>Sina Weibo : @igem_BIT</p>
 +
                        <p>Website : <a href="javascript:;">http://www.bit.edu.cn</a></p>
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Revision as of 04:47, 18 September 2015

iGem_BIT

Disease Alarm

Description

  1. Home
  2. Description

BIOLOGY

Improvement

Compared to last year’s project, the project this year brings in a new amplifying system in signal amplifying for microbe, which significantly improves the efficiency of signal amplifying. For the feature of project, we build a cascade amplifier in microbe by imitating cascade amplifier in circuitry, based on amplifying system of last year.

Last year: In the plasmid of amplification system, two activating transcription factors are in constant expression and remain in a certain concentration. With a certain level of autoinducer PAO1, PAO1 integrates with lasR, the complex of which are bond to the target site of las promoter, then activating it and control the expression of the downstream reporter gene GFP in accord with the concentration of the amount of combination.

This year: Inside the upriver bacteria, certain enzyme will digest the oligopeptides that is from glycopeptide so that the arabinose will be released and induce the engineering bacteria expressing LasI protein. The protein can catalyze matrix producing a large number of small molecules PAI1. When PAI1 free diffuse into the downstream bacteria, it will combine with the constitutive expressed LasR protein and form a transcription factor complex. The combination between complex and the pLas promoter accelerate the process of transcription and translation to generate the Rhl1 enzyme, which can catalyze producing the autoinducer PAI2 from system RhIIR. The complex of PAI2 and RhIR protein that is mediated-expressed by double promoter in bacteria can act on pRhl promoter and induce the engineering bacteria to express GFP.

Innovation

We use aptamers as intermedia innovatively, which forms a competitive inhibition system, so as to realize the goal this year that detecting biochemical macromolecules by microbe.

This year: Outside bacteria, we combine the artificial oligopeptides with the aptamers. As the macromolecules get into the measurement system, they will take places of glycopeptide and leave them as dissociated molecules. Finally, these free glycopeptide will enter the transformed engineering bacteria.

HARDWARE

Improvement

For fluorescence detection device, the improvement are as following. First of all, we use blue laser pen instead of blue LED light, for its light concentrates better and the energy is more powerful, which remarkably improves the efficiency of fluorescent protein stimulation. Secondly, given that fluorescent chromaticity is single, we use sensor OPT101 instead of sensor TCS32XX to improve accuracy, which is sensitive to grayscale rather than chromaticity. Finally, we use UART screen instead of LED panel, which sharply improves the user interaction interface and is more interactive.

Innovation

In order to make microbe express fluorescent protein rapidly, we innovatively build a bacterial culture device, which refers to thermostatic shaker in laboratory. The details is shown in “Hardware-Design”.

contact info

Address: Beijing Institute of Technology, No. 5 South Zhong Guan Cun Street, Haidian Beijing 100081, P. R. China

Twitter : @igem_BIT

Sina Weibo : @igem_BIT

Website : http://www.bit.edu.cn

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