Difference between revisions of "Team:Westminster/Results"

Line 6: Line 6:
  
 
<h3> Project Results</h3>
 
<h3> Project Results</h3>
 +
RESULTS<br>
 +
<b>11/09/15</b><br>
 +
Nanodrop results from plasmid prep<br>
 +
OmcA (1) - 260/280= 1.87 260/230=2.10<br>
 +
TOTAL= 114.5 ng/µl<br>
 +
OmcA (2) - 260/280= 1.88 260/230=2.13<br>
 +
TOTAL= 77.9 ng/µl<br>
 +
CymA (1) - 260/280= 1.86 260/230=2.13<br>
 +
TOTAL= 183.5ng/µl<br>
 +
CymA (2) - 260/280= 1.88 260/230=2.06<br>
 +
TOTAL= 107.1ng/µl<br>
 +
MtrC (1) - 260/280= 1.88 260/230=2.15<br>
 +
TOTAL= 166.7ng/µl<br>
 +
MtrC (2) - 260/280= 1.89 260/230=2.18<br>
 +
TOTAL= 91.1 ng/µl<br>
 +
<b>17/09/15<br>
 +
2 Gels of PCR products extracted yesterday morning (16/09/15) (tube 1, 2, 3, 4, 5, 6, 7, 8, MtrCAB). Gel was run for 40 mins at 100V. <br>
 +
GEL 1</b><br>
 +
<table border=="1" >
 +
<tbody>
 +
<tr>
 +
<td valign="top" ><p><b>Lane 1</b></p></td>
 +
<td valign="top" ><p><b>Lane 2</b></p></td>
 +
<td valign="top" ><p><b>Lane 3</b></p></td>
 +
<td valign="top" ><p><b>Lane 4</b></p></td>
 +
<td valign="top" ><p><b>Lane 5</b></p></td>
 +
<td valign="top" ><p><b>Lane 6</b></p></td>
 +
<td valign="top" ><p><b>Lane 7</b></p></td>
 +
<td valign="top" ><p><b>Lane 8</b></p></td>
 +
</tr>
  
−
<p>Here you can describe the results of your project and your future plans. </p>
+
<tr>
 +
<td valign="top" ><p><b>DNA ladder (10</b> <b>µl)</b></p></td>
 +
<td valign="top" ><p><b>MtrCAB (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>Tube 1 (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>Tube 2 (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>Tube 3 (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>Tube 4 (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>DNA ladder (10</b> <b>µl)</b></p></td>
 +
<td valign="top" ><p><b>Empty</b></p></td>
 +
</tr>
  
−
<h5>What should this page contain?</h5>
+
</tbody>
−
<ul>
+
</table>
−
<li> Clearly and objectively describe the results of your work.</li>
+
−
<li> Future plans for the project </li>
+
−
<li> Considerations for replicating the experiments </li>
+
−
</ul>
+
  
  
 +
<br><br>Both ladders and MtrCAB were the only lanes with results.  <br>
  
 +
<br><br> <br> <br> <br> <br> <br>image here <br> <br> <br> <br> <br> <br>
  
  
−
<h3> Project Achievements </h3>
 
  
−
<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
  
−
<ul>
+
<b>GEL 2 </b><br><table border="1">  
−
<li>A list of linked bullet points of the successful results during your project</li>
+
<tbody>
−
<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
+
<tr>
−
</ul>
+
<td valign="top" ><p><b>Lane 1</b></p></td>
 +
<td valign="top" ><p><b>Lane 2</b></p></td>
 +
<td valign="top" ><p><b>Lane 3</b></p></td>
 +
<td valign="top" ><p><b>Lane 4</b></p></td>
 +
<td valign="top" ><p><b>Lane 5</b></p></td>
 +
<td valign="top" ><p><b>Lane 6</b></p></td>
 +
<td valign="top" ><p><b>Lane 7</b></p></td>
 +
<td valign="top" ><p><b>Lane 8</b></p></td>
 +
</tr>
  
 +
<tr>
 +
<td valign="top" ><p><b>DNA ladder (10</b> <b>µl)</b></p></td>
 +
<td valign="top" ><p><b>Tube 5 (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>Tube 6 (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>Tube 7 (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>Tube 8 (2 µl + 8 µl)</b></p></td>
 +
<td valign="top" ><p><b>DNA ladder (10</b> <b>µl)</b></p></td>
 +
<td valign="top" ><p><b>Empty</b></p></td>
 +
<td valign="top" ><p><b>Empty</b></p></td>
 +
</tr>
  
 +
</tbody>
 +
</table>
  
−
<h3>Inspiration</h3>
+
 
−
<p>See how other teams presented their results.</p>
+
<br>
−
<ul>
+
Lane 3 was the only lane without results. 
−
<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
+
<br> <br> <br> <br> <br> <br>image here <br> <br> <br> <br> <br> <br>
−
<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
+
 
−
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
+
 
−
</ul>
+
<br>
 +
<b>Plasmid prep</b> <br>
 +
9 LB bottles were inoculated with amplicillin (100µg/ml) or chlorophenicol (34.5µg/ml) and plasmid pSB1C3 or pSB1A3 from yesterday’s transformation and left to incubate overnight (pSB1C3 =tube 2, 4, 5, 7 and MtrCAB; pSB1A3= tube 1, 3, 6, 8).<br><br>
 +
 
 +
<table border="1">
 +
<tbody>
 +
<tr>
 +
<td valign="top" ><p>Steps</p></td>
 +
<td valign="top" ><p>Tube 1,3,6,8 (Amp)</p></td>
 +
<td valign="top" ><p>Tube 2,5,7 (all Chlr) MtrCAB, </p></td>
 +
<td valign="top" ><p>Tube 4 Chlr</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>1. Harvest</p></td>
 +
<td valign="top" ><p>Yes 15mins + extra 5 mins</p></td>
 +
<td valign="top" ><p>Yes 15mins + extra 5 mins</p></td>
 +
<td valign="top" ><p>Yes 15mins + extra 5 mins</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>2. Resuspend</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>3. Lyse</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>4. Precipitate</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>5. Bind</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>6. Wash (optional)</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>7. Wash and Ethanol Removal</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>8. Elute</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p>9. Recover</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
<td valign="top" ><p>yes</p></td>
 +
</tr>
 +
 
 +
</tbody>
 +
</table>
 +
 
 +
<br>
 +
 
 +
 
 +
Tube 3 (Amp) - resuspended with 340 µl rather than 250 µl <br>
 +
Tube 7 (Chlr) - 360 µl resuspension buffer added rather than 250 µl <br>
 +
<b>Nanodrop results of plasmid prep </b><br>
 +
<b>Amp 1</b>- 260/280= 1.98 260/230=2.12 <br>
 +
TOTAL= 83.0ng/µl <br>
 +
<b>Chlr 2</b>- 260/280= 1.89 260/230=2.11 <br>
 +
TOTAL= 151.7ng/µl <br>
 +
<b>Amp 3</b>- 260/280= 1.91 260/230=2.12 <br>
 +
TOTAL= 173.5ng/µl <br>
 +
<b>Chlr 4</b>- 260/280= 1.89 260/230=2.18 <br>
 +
TOTAL= 92.1ng/µl <br>
 +
<b>Chlr 5</b>- 260/280= 1.93 260/230=2.23 <br>
 +
TOTAL= 87.8ng/µl <br>
 +
<b>Amp 6</b>- 260/280= 1.92 260/230=2.15 <br>
 +
TOTAL= 129.5ng/µl <br>
 +
<b>Chlr 7</b>- 260/280= 1.91 260/230=2.21 <br>
 +
TOTAL= 80.4ng/µl <br>
 +
<b>Chlr 8</b>- 260/280= 1.94 260/230=2.11 <br>
 +
TOTAL= 128.4ng/µl <br>
 +
<b>MtrCAB</b>- 260/280= 1.96 260/230=2.28 <br>
 +
TOTAL= 38.3ng/µl <br> <br>
 +
 
 +
Below is the plate that the plasmid DNA that will be freeze dried <br>
 +
 
 +
 
 +
 
 +
 
 +
<table border="1">
 +
<tbody>
 +
<tr>
 +
<td valign="top" ><p><br />
 +
</p></td>
 +
<td valign="top" ><p><b>1</b></p></td>
 +
<td valign="top" ><p><b>2</b></p></td>
 +
<td valign="top" ><p><b>3</b></p></td>
 +
<td valign="top" ><p><b>4</b></p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p><b>A</b></p></td>
 +
<td valign="top" ><p>1</p></td>
 +
<td valign="top" ><p>9</p></td>
 +
<td valign="top" ><p>17</p></td>
 +
<td valign="top" ><p>25</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p><b>B</b></p></td>
 +
<td valign="top" ><p>2</p></td>
 +
<td valign="top" ><p>10</p></td>
 +
<td valign="top" ><p>18</p></td>
 +
<td valign="top" ><p>26</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p><b>C</b></p></td>
 +
<td valign="top" ><p>3</p></td>
 +
<td valign="top" ><p>11</p></td>
 +
<td valign="top" ><p>19</p></td>
 +
<td valign="top" ><p>27</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p><b>D</b></p></td>
 +
<td valign="top" ><p>4</p></td>
 +
<td valign="top" ><p>12</p></td>
 +
<td valign="top" ><p>20</p></td>
 +
<td valign="top" ><p><br />
 +
</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p><b>E</b></p></td>
 +
<td valign="top" ><p>5</p></td>
 +
<td valign="top" ><p>13</p></td>
 +
<td valign="top" ><p>21</p></td>
 +
<td valign="top" ><p><br />
 +
</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p><b>F</b></p></td>
 +
<td valign="top" ><p>6</p></td>
 +
<td valign="top" ><p>14</p></td>
 +
<td valign="top" ><p>22</p></td>
 +
<td valign="top" ><p><br />
 +
</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p><b>G</b></p></td>
 +
<td valign="top" ><p>7</p></td>
 +
<td valign="top" ><p>15</p></td>
 +
<td valign="top" ><p>23</p></td>
 +
<td valign="top" ><p><br />
 +
</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td valign="top" ><p><b>H</b></p></td>
 +
<td valign="top" ><p>8</p></td>
 +
<td valign="top" ><p>16</p></td>
 +
<td valign="top" ><p>24</p></td>
 +
<td valign="top" ><p><br />
 +
</p></td>
 +
</tr>
 +
 
 +
</tbody>
 +
</table>
 +
 
 +
<table><tr><td>
 +
1. MtrA pSB1C3 <br>
 +
2. MtrB pSB1C3 <br>
 +
3. MtrC pSB1C3 <br>
 +
4. MtrC (1) <br>
 +
5. MtrC (2) <br>
 +
6. CymA (1) <br>
 +
7. CymA (2) </td><td>
 +
8. OmcA <br>
 +
9. OmcA (1) <br>
 +
10. OmcA (2) <br>
 +
11. MtrA-His <br>
 +
12. MtrB-His <br>
 +
13. MtrC-His <br>
 +
14. CymA-His <br>
 +
15. OmcA-His
 +
16- OmcA-CymA(1)</td><td>
 +
17. OmcA-CymA (2) <br>
 +
18. MtrCAB <br>
 +
19. Chlr 2 <br>
 +
20. Chlr 4 <br>
 +
21. Chlr 5 <br>
 +
22. Chlr 7</td></tr></table>
  
  

Revision as of 07:12, 18 September 2015

Project Results

RESULTS
11/09/15
Nanodrop results from plasmid prep
OmcA (1) - 260/280= 1.87 260/230=2.10
TOTAL= 114.5 ng/µl
OmcA (2) - 260/280= 1.88 260/230=2.13
TOTAL= 77.9 ng/µl
CymA (1) - 260/280= 1.86 260/230=2.13
TOTAL= 183.5ng/µl
CymA (2) - 260/280= 1.88 260/230=2.06
TOTAL= 107.1ng/µl
MtrC (1) - 260/280= 1.88 260/230=2.15
TOTAL= 166.7ng/µl
MtrC (2) - 260/280= 1.89 260/230=2.18
TOTAL= 91.1 ng/µl
17/09/15
2 Gels of PCR products extracted yesterday morning (16/09/15) (tube 1, 2, 3, 4, 5, 6, 7, 8, MtrCAB). Gel was run for 40 mins at 100V.
GEL 1

Lane 1

Lane 2

Lane 3

Lane 4

Lane 5

Lane 6

Lane 7

Lane 8

DNA ladder (10 µl)

MtrCAB (2 µl + 8 µl)

Tube 1 (2 µl + 8 µl)

Tube 2 (2 µl + 8 µl)

Tube 3 (2 µl + 8 µl)

Tube 4 (2 µl + 8 µl)

DNA ladder (10 µl)

Empty


Both ladders and MtrCAB were the only lanes with results.







image here





GEL 2

Lane 1

Lane 2

Lane 3

Lane 4

Lane 5

Lane 6

Lane 7

Lane 8

DNA ladder (10 µl)

Tube 5 (2 µl + 8 µl)

Tube 6 (2 µl + 8 µl)

Tube 7 (2 µl + 8 µl)

Tube 8 (2 µl + 8 µl)

DNA ladder (10 µl)

Empty

Empty

Lane 3 was the only lane without results.





image here






Plasmid prep
9 LB bottles were inoculated with amplicillin (100µg/ml) or chlorophenicol (34.5µg/ml) and plasmid pSB1C3 or pSB1A3 from yesterday’s transformation and left to incubate overnight (pSB1C3 =tube 2, 4, 5, 7 and MtrCAB; pSB1A3= tube 1, 3, 6, 8).

Steps

Tube 1,3,6,8 (Amp)

Tube 2,5,7 (all Chlr) MtrCAB,

Tube 4 Chlr

1. Harvest

Yes 15mins + extra 5 mins

Yes 15mins + extra 5 mins

Yes 15mins + extra 5 mins

2. Resuspend

yes

yes

yes

3. Lyse

yes

yes

yes

4. Precipitate

yes

yes

yes

5. Bind

yes

yes

yes

6. Wash (optional)

yes

yes

yes

7. Wash and Ethanol Removal

yes

yes

yes

8. Elute

yes

yes

yes

9. Recover

yes

yes

yes

Tube 3 (Amp) - resuspended with 340 µl rather than 250 µl
Tube 7 (Chlr) - 360 µl resuspension buffer added rather than 250 µl
Nanodrop results of plasmid prep
Amp 1- 260/280= 1.98 260/230=2.12
TOTAL= 83.0ng/µl
Chlr 2- 260/280= 1.89 260/230=2.11
TOTAL= 151.7ng/µl
Amp 3- 260/280= 1.91 260/230=2.12
TOTAL= 173.5ng/µl
Chlr 4- 260/280= 1.89 260/230=2.18
TOTAL= 92.1ng/µl
Chlr 5- 260/280= 1.93 260/230=2.23
TOTAL= 87.8ng/µl
Amp 6- 260/280= 1.92 260/230=2.15
TOTAL= 129.5ng/µl
Chlr 7- 260/280= 1.91 260/230=2.21
TOTAL= 80.4ng/µl
Chlr 8- 260/280= 1.94 260/230=2.11
TOTAL= 128.4ng/µl
MtrCAB- 260/280= 1.96 260/230=2.28
TOTAL= 38.3ng/µl

Below is the plate that the plasmid DNA that will be freeze dried


1

2

3

4

A

1

9

17

25

B

2

10

18

26

C

3

11

19

27

D

4

12

20


E

5

13

21


F

6

14

22


G

7

15

23


H

8

16

24


1. MtrA pSB1C3
2. MtrB pSB1C3
3. MtrC pSB1C3
4. MtrC (1)
5. MtrC (2)
6. CymA (1)
7. CymA (2) 		8. OmcA
9. OmcA (1)
10. OmcA (2)
11. MtrA-His
12. MtrB-His
13. MtrC-His
14. CymA-His
15. OmcA-His 16- OmcA-CymA(1)		 17. OmcA-CymA (2)
18. MtrCAB
19. Chlr 2
20. Chlr 4
21. Chlr 5
22. Chlr 7