Difference between revisions of "Team:BIOSINT Mexico/Project"

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Results
 
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<br>spisPink & amilGFP optimized to Escherichia coli K12 </br>
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<br>After completing the insertion of gBlocks (IDT) designed (spisPink and amilGFP), into the plasmid pSB1C3, by following the relevant protocols; competent cells were successfully transformed and consequently the removal of plasmids (spisPink into pSB1C3) BBa_K1684000 and (amilGFP into pSB1C3) BBa_K1684001 was performed.</br>
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<br>The Figure 6 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 1, 2 and 3 are located the triplicate samples of spisPink piece, optimized for E. coli K12 (BBa_K1684000), with a size of 2789 bp. Likewise on the line 4,5 and 6 are repeated samples of the piece amilGFP optimized for E. coli K12 (BBa_K1684001). For both BioBricks, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.</br>
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Revision as of 07:19, 18 September 2015

Coliroid

Background

Overview

Color Coliroid

Assembly

Methods

Light Cannon

Results


spisPink & amilGFP optimized to Escherichia coli K12

After completing the insertion of gBlocks (IDT) designed (spisPink and amilGFP), into the plasmid pSB1C3, by following the relevant protocols; competent cells were successfully transformed and consequently the removal of plasmids (spisPink into pSB1C3) BBa_K1684000 and (amilGFP into pSB1C3) BBa_K1684001 was performed.

The Figure 6 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 1, 2 and 3 are located the triplicate samples of spisPink piece, optimized for E. coli K12 (BBa_K1684000), with a size of 2789 bp. Likewise on the line 4,5 and 6 are repeated samples of the piece amilGFP optimized for E. coli K12 (BBa_K1684001). For both BioBricks, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.