Difference between revisions of "Team:TU Darmstadt/Notebook/sec1/K1602040"
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<a href=" https://2015.igem.org/Team:TU_Darmstadt/Notebook/sec1/K1602037 " title="Opens internal link in current window" class="internal link">K1602037</a> was digested with <em>Xba</em>I and <em>Pst</em>I and ligated into T7-pSB1A2. Competent <em>E. coli</em> Top 10 were transformed via heat shock. The plasmids were extracted and digested with <em>EcoR</em>I and <em>Pst</em>I and cloned into pSB1C3. Competent <em>E. coli</em> Top 10 were transformed and colonies were screened by colony PCR. Plasmids were extracted and used for protein expression in <em>E. coli</em> strain BL21. </p> | <a href=" https://2015.igem.org/Team:TU_Darmstadt/Notebook/sec1/K1602037 " title="Opens internal link in current window" class="internal link">K1602037</a> was digested with <em>Xba</em>I and <em>Pst</em>I and ligated into T7-pSB1A2. Competent <em>E. coli</em> Top 10 were transformed via heat shock. The plasmids were extracted and digested with <em>EcoR</em>I and <em>Pst</em>I and cloned into pSB1C3. Competent <em>E. coli</em> Top 10 were transformed and colonies were screened by colony PCR. Plasmids were extracted and used for protein expression in <em>E. coli</em> strain BL21. </p> | ||
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Latest revision as of 07:38, 18 September 2015
K1602040 - T7-B0034-enhanced yellow fluorescent protein-GBD-ligand (T7-B0034-eYFP-GBDlig)
K1602037 was digested with XbaI and PstI and ligated into T7-pSB1A2. Competent E. coli Top 10 were transformed via heat shock. The plasmids were extracted and digested with EcoRI and PstI and cloned into pSB1C3. Competent E. coli Top 10 were transformed and colonies were screened by colony PCR. Plasmids were extracted and used for protein expression in E. coli strain BL21.