|
|
| Line 1: |
Line 1: |
| − | {{Template_All_Teams}}
| |
| − | <html>
| |
| − | </div></div></div></div></div></div></div></div></div></div>
| |
| − | </html>
| |
| − | {{Team:UMarylandClasses}}
| |
| − | {{Team:UMarylandMenu}}
| |
| − | <html>
| |
| | | | |
| − | <!--Attention! If you are not part of the website team, you are NOT allowed to touch anything above this line without the express permission of Best Kohai.-->
| |
| − |
| |
| − | <style type="text/css">
| |
| − |
| |
| − | h1,h2,h3,h4,p,a {
| |
| − | text-decoration: none;
| |
| − | }
| |
| − | h1 {
| |
| − | font-size: 250%;
| |
| − | }
| |
| − | h2 {
| |
| − | font-size: 200%;
| |
| − | }
| |
| − | h3 {
| |
| − | font-size: 150%;
| |
| − | }
| |
| − | h4 {
| |
| − | font-size: 125%;
| |
| − | }
| |
| − | p {
| |
| − | font-size: 100%;
| |
| − | }
| |
| − | #sidemenu {
| |
| − | position:fixed;
| |
| − | float:left;
| |
| − | width: 200px;
| |
| − | top:200px;
| |
| − | }
| |
| − |
| |
| − | #sidemenu,
| |
| − | #sidemenu ul,
| |
| − | #sidemenu ul li,
| |
| − | #sidemenu ul li a {
| |
| − | margin: 0;
| |
| − | padding: 0;
| |
| − | border: 0;
| |
| − | list-style: none;
| |
| − | line-height: 1;
| |
| − | display: block;
| |
| − | -webkit-box-sizing: border-box;
| |
| − | -moz-box-sizing: border-box;
| |
| − | box-sizing: border-box;
| |
| − | }
| |
| − |
| |
| − | #sidemenu ul ul {
| |
| − | display: none;
| |
| − | }
| |
| − | .align-right {
| |
| − | float: right;
| |
| − | }
| |
| − | #sidemenu > ul > li > a {
| |
| − | padding: 15px 20px;
| |
| − | border: 1px solid black;
| |
| − | cursor: pointer;
| |
| − | z-index: 2;
| |
| − | font-size: 14px;
| |
| − | font-weight: bold;
| |
| − | text-decoration: none;
| |
| − | color: #000000;
| |
| − | background-image: -webkit-linear-gradient(bottom, #ffffff 0%, #2BB1FF 100%);
| |
| − | box-shadow: inset 0 1px 0 rgba(255, 255, 255, 0.15);
| |
| − | }
| |
| − |
| |
| − | #sidemenu > ul > li > a:hover,
| |
| − | #sidemenu > ul > li.active > a,
| |
| − | #sidemenu > ul > li.open > a {
| |
| − | color: #000000;
| |
| − | background-image: -webkit-linear-gradient(top, #C9C9C9 0%, #FFFFFF 100%);
| |
| − | }
| |
| − | #sidemenu > ul > li.open > a {
| |
| − | box-shadow: inset 0 1px 0 rgba(255, 255, 255, 0.15), 0 1px 1px rgba(0, 0, 0, 0.15);
| |
| − | border-bottom: 1px solid #1682ba;
| |
| − | }
| |
| − |
| |
| − |
| |
| − | #sidemenu > ul > li > a:hover > span::after,
| |
| − | #sidemenu > ul > li.active > a > span::after,
| |
| − | #sidemenu > ul > li.open > a > span::after {
| |
| − | border-color: #eeeeee;
| |
| − | }
| |
| − | .holder::before {
| |
| − | top: 18px;
| |
| − | border-top: 2px solid;
| |
| − | border-left: 2px solid;
| |
| − | border-top-color: inherit;
| |
| − | border-left-color: inherit;
| |
| − | }
| |
| − | #sidemenu ul ul li a {
| |
| − | cursor: pointer;
| |
| − | border-bottom: 1px solid #32373e;
| |
| − | border-left: 1px solid #32373e;
| |
| − | border-right: 1px solid #32373e;
| |
| − | padding: 10px 20px;
| |
| − | z-index: 1;
| |
| − | text-decoration: none;
| |
| − | font-size: 13px;
| |
| − | color: #eeeeee;
| |
| − | background: #49505a;
| |
| − | box-shadow: inset 0 1px 0 rgba(255, 255, 255, 0.1);
| |
| − | }
| |
| − | #sidemenu ul ul li:hover > a,
| |
| − | #sidemenu ul ul li.open > a,
| |
| − | #sidemenu ul ul li.active > a {
| |
| − | background: #424852;
| |
| − | color: #ffffff;
| |
| − | }
| |
| − | #sidemenu ul ul li:first-child > a {
| |
| − | box-shadow: none;
| |
| − | }
| |
| − | #sidemenu ul ul ul li:first-child > a {
| |
| − | box-shadow: inset 0 1px 0 rgba(255, 255, 255, 0.1);
| |
| − | }
| |
| − | #sidemenu ul ul ul li a {
| |
| − | padding-left: 30px;
| |
| − | }
| |
| − | #sidemenu > ul > li > ul > li:last-child > a,
| |
| − | #sidemenu > ul > li > ul > li.last > a {
| |
| − | border-bottom: 0;
| |
| − | }
| |
| − | #sidemenu > ul > li > ul > li.open:last-child > a,
| |
| − | #sidemenu > ul > li > ul > li.last.open > a {
| |
| − | border-bottom: 1px solid #32373e;
| |
| − | }
| |
| − | #sidemenu > ul > li > ul > li.open:last-child > ul > li:last-child > a {
| |
| − | border-bottom: 0;
| |
| − | }
| |
| − | #sidemenu ul ul li.has-sub > a::after {
| |
| − | display: block;
| |
| − | position: absolute;
| |
| − | content: "";
| |
| − | width: 5px;
| |
| − | height: 5px;
| |
| − | right: 20px;
| |
| − | z-index: 10;
| |
| − | top: 11.5px;
| |
| − | border-top: 2px solid #eeeeee;
| |
| − | border-left: 2px solid #eeeeee;
| |
| − | -webkit-transform: rotate(-135deg);
| |
| − | -moz-transform: rotate(-135deg);
| |
| − | -ms-transform: rotate(-135deg);
| |
| − | -o-transform: rotate(-135deg);
| |
| − | transform: rotate(-135deg);
| |
| − | }
| |
| − | #sidemenu ul ul li.active > a::after,
| |
| − | #sidemenu ul ul li.open > a::after,
| |
| − | #sidemenu ul ul li > a:hover::after {
| |
| − | border-color: #ffffff;
| |
| − | }
| |
| − |
| |
| − | #layer1 {
| |
| − | overflow:hidden;
| |
| − | position:relative;
| |
| − | width:100%;
| |
| − | margin:0px;
| |
| − | padding:20px;
| |
| − | background-color: #89e1ff;
| |
| − | min-height:42px;
| |
| − | top:-245px;
| |
| − | border-top:2px solid black;
| |
| − | }
| |
| − | #layer2 {
| |
| − | overflow:hidden;
| |
| − | position:relative;
| |
| − | width:100%;
| |
| − | margin:0px;
| |
| − | padding:20px;
| |
| − | background-color:#ffffff;
| |
| − | min-height:42px;
| |
| − | top:-245px;
| |
| − | border-top:2px solid black;
| |
| − | }
| |
| − | #layer3 {
| |
| − | overflow:hidden;
| |
| − | position:relative;
| |
| − | width:100%;
| |
| − | margin:0px;
| |
| − | padding:20px;
| |
| − | background-color:#7fcff9;
| |
| − | min-height:42px;
| |
| − | top:-245px;
| |
| − | border-top:2px solid black;
| |
| − | }
| |
| − | #layer4 {
| |
| − | overflow:hidden;
| |
| − | position:relative;
| |
| − | width:100%;
| |
| − | margin:0px;
| |
| − | padding:20px;
| |
| − | background-color:#ffffff;
| |
| − | min-height:42px;
| |
| − | }
| |
| − | #contentbox{
| |
| − | width: 80%;
| |
| − | max-width: 1900px;
| |
| − | min-width: 1000px;
| |
| − | text-align: justify;
| |
| − | margin:auto;
| |
| − | font-family: "Palatino Linotype", "Book Antiqua", Palatino, serif;
| |
| − | }
| |
| − | #cover {
| |
| − | overflow:hidden;
| |
| − | position:relative;
| |
| − | width:100%;
| |
| − | margin:auto;
| |
| − | padding:0px;
| |
| − | background-image: url("https://static.igem.org/mediawiki/2015/9/97/Resultsq.png");
| |
| − | background-size: 100% ;
| |
| − | background-repeat: no-repeat;
| |
| − | height: 100%;
| |
| − | width:100%;
| |
| − | min-height:800px;
| |
| − | color: #0DFF00;
| |
| − | font-family: Verdana, Geneva, sans-serif;
| |
| − | font-size:xx-large;
| |
| − | top:-200px;
| |
| − | }
| |
| − | #buttonset {
| |
| − | text-align:center;
| |
| − | bottom:0px;
| |
| − | position:relative;
| |
| − | top:500px;
| |
| − | }
| |
| − |
| |
| − |
| |
| − | #bar{
| |
| − | display: inline-block;
| |
| − | opacity:1;
| |
| − | background-color: rgba(255,255,255,.5);
| |
| − | color:black;
| |
| − | border-top:4px solid black;
| |
| − | border-bottom:4px solid black;
| |
| − | width:100%;
| |
| − | text-align:center;
| |
| − | height:200px;
| |
| − | position:absolute;
| |
| − | top:200px;
| |
| − | text-shadow: 4px 4px 4px white;
| |
| − | }
| |
| − | </style>
| |
| − |
| |
| − | <div id='cover'>
| |
| − | <div id='bar'>
| |
| − | <p style="font-size:64px"><b>Notebook</b>
| |
| − | </div>
| |
| − | <div id='sidemenu'>
| |
| − | <ul>
| |
| − | <li><a href="#Week1" class="smoothScroll"><span>Week 1</span></a></li>
| |
| − | <li><a href="#Week2" class="smoothScroll"><span>Week 2</span></a></li>
| |
| − | <li><a href="#Week3" class="smoothScroll"><span>Week 3</span></a></li>
| |
| − | <li><a href="#Week4" class="smoothScroll"><span>Week 4</span></a></li>
| |
| − | <li><a href="#Week5" class="smoothScroll"><span>Week 5</span></a></li>
| |
| − | <li><a href="#Week6" class="smoothScroll"><span>Week 6</span></a></li>
| |
| − | <li><a href="#Week7" class="smoothScroll"><span>Week 7</span></a></li>
| |
| − | <li><a href="#Week8" class="smoothScroll"><span>Week 8</span></a></li>
| |
| − | <li><a href="#Week9" class="smoothScroll"><span>Week 9</span></a></li>
| |
| − | <li><a href="#Week10" class="smoothScroll"><span>Week 10</span></a></li>
| |
| − | <li><a href="#Week11" class="smoothScroll"><span>Week 11</span></a></li>
| |
| − | <li><a href="#Week12" class="smoothScroll"><span>Week 12</span></a></li>
| |
| − | <li><a href="#Week13" class="smoothScroll"><span>Week 13</span></a></li>
| |
| − | <li><a href="#Week14" class="smoothScroll"><span>Week 14</span></a></li>
| |
| − | <li><a href="#Week15" class="smoothScroll"><span>Week 15</span></a></li>
| |
| − | <li><a href="#Week16" class="smoothScroll"><span>Week 16</span></a></li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − | <div id='layer2'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week1">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 1</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − | ligated the pBAD promoter in PSB1C3 with the miraculin gene in PSB1C3 using 3A assembly
| |
| − | plate had colonies
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer1'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week2">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 2</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − | pBAD +miraculin construct was moved back into psb1C3 backbone and sent for sequencing
| |
| − | Designing gBlocks:
| |
| − | Designed gblocks for epsilon-cyclase (from arabidopsis), pyocin and Hok/Sok
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer2'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week 3">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week3</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − | sequence confirmed through sequencing
| |
| − | failed to extract using French press, FPLC and SDS-Page
| |
| − | could be due to high arabinose induction (OD of 1)
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id='layer1'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week4">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 4</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − | induced 3 test cultures with 0%, 0.05%, 0.1%, 0.2% arabinose with no significant results
| |
| − | will perform procedure again using bl21 instead of Dh5a
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer2'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week5">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 5</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin
| |
| − | transformation in BL21 strain was successful
| |
| − | SDS page showed a band at roughly 25 kDa
| |
| − | Hok/Sok
| |
| − | inserted Hok/Sok gblock into PSB1C3 backbone using Gibson assembly
| |
| − | construct sent for sequencing
| |
| − | Interlab Study
| |
| − | transformed parts into dh5a: 3 Anderson promoters, GFP, and RFP
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id='layer1'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week6">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 6</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − | induced 3 test cultures with 0%, 0.05%, 0.1%, 0.2% arabinose with no significant results
| |
| − | will perform procedure again using bl21 instead of Dh5a
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id='layer2'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week7">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 7</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer1'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week8">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 8</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer2'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week9">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 9</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer1'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week10">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 10</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer2'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week11">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 11</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer1'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week12">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 12</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer2'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week13">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 13</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − |
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer1'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week14">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 14</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − |
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer2'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week15">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 15</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − |
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div id='layer1'>
| |
| − | <div id='contentbox'>
| |
| − | <a name="Week16">
| |
| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
| |
| − | <b>Week 16</b></a>
| |
| − | <p style="font-size:24px;text-align:center;">
| |
| − | Miraculin:
| |
| − |
| |
| − |
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| − |
| |
| − | </html>
| |