Difference between revisions of "Team:UAM Poznan"

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<h2> EcoFactory, a multipromoter explorer expression system for <i> Escherichia coli </i> </h2>
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<p> At the moment, the most popular system in use for protein expression is the pET expression system.
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The pET expression system is a lactose induced T7 polymerase dependent system.
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This system, however, has few downsides: i) the system tends to leak; ii) it is very hard to control the protein expression level once it is induced; iii) being a viral polymerase,T7 polymerase has a tendency to insert the mismatches (mutations) in the transcripted sequence; iv) if the produced protein is toxic for the host, the bacterial culture can decline before the desired concentration of the protein of interest is manufactured; v) the organized and stable transfer of the produced protein to i.e. a medium can prove difficult to carry out. </p>
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<p> Therefore, we decided to seek an alternate solution to use in protein expression.
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We prepared a series of plasmids expressing his-tagged superfolder GFP under the control of four nontoxic inducers: arabinose, melibiose, rhamnose and xylose.  The four promoters are more controllable than the most popular lactose induced T7RNA polymerase dependent systems. The coding sequences are transcribed by the cellular RNA polymerase which is slower and more accurate than T7 RNA polymerase.  SfGFP can be used to compare activities of standard, modified, and minimal promoters during growth on different carbon sources and as well as a probe of a fused protein solubility. We present a  comprehensive comparison of the sfGFP expression driven by inducible promoters with the insulated, constitutive promoters proC and proD as standards. Two pairs of primers  should be sufficient to clone an ORF of interest as a protein fused to sfGFP or to replace it in all vectors of the mulltipromoter system. </p>
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<p> Our next step is to compare the efficiency between these promoters and test their function when grown on the media consisting of different components.
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<h4>Before you start: </h4>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<h4> Styling your wiki </h4>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h4> Editing your wiki </h4>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:UAM_Poznan&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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<h4>Templates </h4>
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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<h4>Tips</h4>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h4>Inspiration</h4>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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<a target="_blank"href="https://2015.igem.org/Team:UAM_Poznan"><img class="banner" src="https://static.igem.org/mediawiki/2015/4/4a/UAMPOZNANbannerhorizontal.png" style="display:block; margin-left: auto; margin-right:auto;" border="1"></a>
  
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  <div class="column-center"><a target="_blank"href="https://2015.igem.org/Team:UAM_Poznan/Parts"><img class="button" src="https://static.igem.org/mediawiki/2015/2/27/UAMPOZNANparts.jpg" ></a></div>
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  <div class="column-left"><a target="_blank"href="https://2015.igem.org/Team:UAM_Poznan/Events"><img class="button" src="https://static.igem.org/mediawiki/2015/4/47/UAMPOZNANevents.jpg" ></a></div>
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<img src="">: igem2015uam@gmail.com
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Revision as of 11:05, 18 September 2015

: igem2015uam@gmail.com