Difference between revisions of "Team:CityU HK/Experiments"

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<div class="paragraph" style="text-align:left;"><font size="3"><span style="">The lysis plasmid mainly is consisted of two parts: the lysis cassette and Lac repressor gene. </span><br /><span style=""></span><br /><span style=""></span>  <span "font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:="" &quot;calibri&quot;,&quot;sans-serif&quot;;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:="" &#26032;&#32048;&#26126;&#39636;;mso-fareast-theme-font:minor-fareast;mso-hansi-theme-font:minor-latin;="" mso-bidi-font-family:&quot;times="" roman&quot;;mso-bidi-theme-font:minor-bidi;="" mso-ansi-language:en-us;mso-fareast-language:zh-tw;mso-bidi-language:ar-sa"="" style="">The lysis cassette is composed of holin (<em style="">S</em> gene), endolysin (<em style="">R</em> gene) and spanin (<em style="">Rz</em> gene). Two phage origins of the lysis cassette, phage lambda and phage 21, were cloned and compared. To speed up the cell lysis for releasing &beta;-galactosidase, 2 modifications including codon optimization and mutations on the <em style="">S </em>gene were included. A missense mutation and a deletion of the trans-membrane domain were applied to the <em style="">S</em> gene of phage lambda and phage 21, respectively. Our team has constructed 9 lysis cassettes with different combinations (please refer to the <a href="https://static.igem.org/mediawiki/2015/4/41/2015CityU_HK_Interlab_Sequencing.pdf" style="color: #000000"><b><u>PARTS</u></b></a>). For all the cassettes, pL8-UV5, a LacI regulated promoter was used to turn the transcription on.</span></font></div>
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<div class="paragraph" style="text-align:left;"><font size="3"><span style="">The lysis plasmid mainly is consisted of two parts: the lysis cassette and Lac repressor gene. </span><br /><span style=""></span><br /><span style=""></span>  <span "font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:="" &quot;calibri&quot;,&quot;sans-serif&quot;;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:="" &#26032;&#32048;&#26126;&#39636;;mso-fareast-theme-font:minor-fareast;mso-hansi-theme-font:minor-latin;="" mso-bidi-font-family:&quot;times="" roman&quot;;mso-bidi-theme-font:minor-bidi;="" mso-ansi-language:en-us;mso-fareast-language:zh-tw;mso-bidi-language:ar-sa"="" style="">The lysis cassette is composed of holin (<em style="">S</em> gene), endolysin (<em style="">R</em> gene) and spanin (<em style="">Rz</em> gene). Two phage origins of the lysis cassette, phage lambda and phage 21, were cloned and compared. To speed up the cell lysis for releasing &beta;-galactosidase, 2 modifications including codon optimization and mutations on the <em style="">S </em>gene were included. A missense mutation and a deletion of the trans-membrane domain were applied to the <em style="">S</em> gene of phage lambda and phage 21, respectively. Our team has constructed 9 lysis cassettes with different combinations (please refer to the <a href="https://2015.igem.org/Team:CityU_HK/Parts" style="color: #000000"><b><u>PARTS</u></b></a>). For all the cassettes, pL8-UV5, a LacI regulated promoter was used to turn the transcription on.</span></font></div>
  
 
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Revision as of 11:17, 18 September 2015

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