Difference between revisions of "Team:IIT Delhi/modelling"
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<h1 style="font-family:'Trebuchet MS', 'Lucida Grande', 'Lucida Sans Unicode', 'Lucida Sans', Tahoma, sans-serif;color:white;font-size:250%;padding-top:18px;">Introduction</h1> | <h1 style="font-family:'Trebuchet MS', 'Lucida Grande', 'Lucida Sans Unicode', 'Lucida Sans', Tahoma, sans-serif;color:white;font-size:250%;padding-top:18px;">Introduction</h1> | ||
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Three of the proteins required in the project are cytochrome c proteins (haem proteins). Hence, the heme biosynthesis pathway needs to be modelled. | Three of the proteins required in the project are cytochrome c proteins (haem proteins). Hence, the heme biosynthesis pathway needs to be modelled. | ||
Pathway of heme production in E. coli (C-5 pathway from glutamate) | Pathway of heme production in E. coli (C-5 pathway from glutamate) | ||
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<h1 style="font-family:'Trebuchet MS', 'Lucida Grande', 'Lucida Sans Unicode', 'Lucida Sans', Tahoma, sans-serif;color:white;font-size:250%;padding-top:18px;">Mathematical model</h1> | <h1 style="font-family:'Trebuchet MS', 'Lucida Grande', 'Lucida Sans Unicode', 'Lucida Sans', Tahoma, sans-serif;color:white;font-size:250%;padding-top:18px;">Mathematical model</h1> | ||
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Revision as of 11:20, 18 September 2015
IntroductionThree of the proteins required in the project are cytochrome c proteins (haem proteins). Hence, the heme biosynthesis pathway needs to be modelled. Pathway of heme production in E. coli (C-5 pathway from glutamate) |
Mathematical modelAlso,d-Aminolevulinate (ALA) produced as an intermediate in the above reactions. -Aminolevulinate Synthase (ALA Synthase) is the committed step of the heme synthesis pathway, and is usually rate-limiting for the overall pathway. Regulation occurs through control of gene transcription. Heme functions as a feedback inhibitor, repressing transcription of the gene for -Aminolevulinate Synthase. |
A non-competitive irreversible feedback inhibition model is assumed for this step with a ki value of 0.02 mM. Hence, the following equation governs the conversion to ALA: |
Click here to read more: |
