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| <ul class="article-ul"> | | <ul class="article-ul"> |
| <li>Microcapsule</li> | | <li>Microcapsule</li> |
− | <p class="article-p">First mechanism is that we will package the E-coli biosensors into microcapsules that prevent E-coli within from leaking out.</p> | + | <p class="article-p">We embed E.coli in the microcapsule by immobilization, which prevent E.coli from polluting environment.</p> |
| <li>Chitosan</li> | | <li>Chitosan</li> |
− | <p class="article-p">Second mechanism is that we have selected one chemical compound- percarbonate that can carry out the ability to sterilize and kill 99% E-coli once activated. | + | <p class="article-p">We solidify chitosan to form a film and put it on the upper and the lower layer of microcapsules, so microcapsules won’t not leak or be destroyed.</p> |
− | </p> | + | |
| <li>Sodium percarbonate</li> | | <li>Sodium percarbonate</li> |
− | <p class="article-p">Third mechanism is that after using this product, one can pour the alimentary acetic acid into the product to activate the sterilizing procedure. | + | <p class="article-p">Sterilizing procedure: By using acetic acid to dissolve chitosan and produce water, the water flows to the lower part and triggers precarbonate, thus activates the sterilizing process.</p> |
− | </p> | + | |
| </ul> | | </ul> |
− | <p class="article-p">Because the alimentary acetic acid can dissolve both layers of chitosan and activate percarbonate for sterilizing. For those three safety mechanisms, we hope to reduce the potential risk that our product might posed and thus can be further utilized by people all over the world.
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− | </p>
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| </article> | | </article> |
| <article class="article"> | | <article class="article"> |
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| <h3>1. Immobilization</h3> | | <h3>1. Immobilization</h3> |
| <h4>1.1 Compare the effect of different immobilization methods on microcapsules’ patterns</h4> | | <h4>1.1 Compare the effect of different immobilization methods on microcapsules’ patterns</h4> |
− | <p>We chose three methods to immobilize E.coli. Then we made a pre-test to ensure each method’ feasibility, including immobilization materials and equipment.</p> | + | <p>We chose three methods to immobilize E.coli. Then we made a pre-test to ensure the feasibility of each method, including immobilization materials and equipment.</p> |
| <h5>1.1.1Procedure</h5> | | <h5>1.1.1Procedure</h5> |
| <h6>PVA(Polyvinyl alcohol)-SA(Alginate)</h6> | | <h6>PVA(Polyvinyl alcohol)-SA(Alginate)</h6> |
| <ol type="a"> | | <ol type="a"> |
| <li>Pour some solution into beakers.</li> | | <li>Pour some solution into beakers.</li> |
− | <li>Draw certain amount of 8%PVA-1%SA and add it into 3%BA(Boric acid)-1%CaCl2.</li> | + | <li>Draw certain amount of 8%PVA-1%SA and add it into 3%BA(Boric acid)-1%CaCl<sub>2</sub>.</li> |
| </ol> | | </ol> |
| <h6>SA(Alginate)</h6> | | <h6>SA(Alginate)</h6> |
| <ol type="a"> | | <ol type="a"> |
| <li>Pour some solution into beakers.</li> | | <li>Pour some solution into beakers.</li> |
− | <li>Draw certain amount of 8%PVA-1%SA and add it into 3%BA(Boric acid)-1%CaCl2.</li> | + | <li>Draw certain amount of 8%PVA-1%SA and add it into 3%BA(Boric acid)-1%CaCl<sub>2</sub>.</li> |
| </ol> | | </ol> |
| <h6>ACA(Alginate & Chitosan)</h6> | | <h6>ACA(Alginate & Chitosan)</h6> |
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| <h5>1.1.2 Result</h5> | | <h5>1.1.2 Result</h5> |
| <h6>1. Different materials of immobilization</h6> | | <h6>1. Different materials of immobilization</h6> |
− | <p>We decided to apply only SA and ACA materials to our experiment, and we will delete step e. of ACA because it’s infeasible.</p> | + | <p>We decided to apply only SA and ACA materials in our experiment, and we deleted step e. of ACA because it’s infeasible.</p> |
| <div class="three-img"> | | <div class="three-img"> |
| <div class="img-figure"> | | <div class="img-figure"> |
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| </div> | | </div> |
| <h6>2. Comparison of syringe and pipet</h6> | | <h6>2. Comparison of syringe and pipet</h6> |
− | <p>We decide to apply syringe to our experiment because syringe has a better effect on microcapsules’ patterns than pipet.</p> | + | <p>We decide to apply syringe in our experiment because it has a better effect on microcapsules’ patterns than pipet.</p> |
| <div class="double-img"> | | <div class="double-img"> |
| <div class="img-figure"> | | <div class="img-figure"> |
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| </ol> | | </ol> |
| <h5>2.1.2 Result</h5> | | <h5>2.1.2 Result</h5> |
| + | <p>The freeze-dried beads have better adsorption to copper</p> |
| <p class="table-note">▼Table 1: The effect of freeze-dried and oven-dried chitosan on Cu(II) ion adsorption</p> | | <p class="table-note">▼Table 1: The effect of freeze-dried and oven-dried chitosan on Cu(II) ion adsorption</p> |
| <table id="table-one"> | | <table id="table-one"> |