Difference between revisions of "Team:UiOslo Norway/Experiments/Restriction Digest"
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Latest revision as of 12:10, 18 September 2015
Restriction Digest:
Back to Protocols5 μl Cutsmart x μl DNA (1 μg total concentration) 1 μl Restriction Enzyme I 1 μl Restriction Enzyme II up to 50 μl MilliQ Water
Total Reaction of 50 μl
Incubate for 1 hour at 37 °C For restriction digests including NdeI, at first only 1 μl of NdeI was added and the digest incubated at 37 °C for 1 hour, afterwards NdeI was heat inactivated at 65 °C for 20 minutes and then the second restriction enzyme was added and the digest was again incubated for 1 hour.
iGEM UiOslo 2015 is sponsored by:
