Difference between revisions of "Team:Pasteur Paris/Week 10"
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− | + | <h3><em>Toxicity and solubility of TPA (Terephthalic acid)</em></h3><br> | |
<ol> | <ol> | ||
<li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li> | <li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li> | ||
<li style="text-align:left">Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l </li> | <li style="text-align:left">Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l </li> | ||
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− | + | Solubilisation of TPA by NaOH | |
+ | Creation of a stock solution of soluble TPA in water at 0,5mol/L | ||
+ | Test of the TPA Solubilisation in LB Broth | ||
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+ | Preparation of different media containing TPA and EG : | ||
+ | Media 1 to 10 are made in solid form | ||
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+ | <!-- <u>3 solutions: </u><br /> | ||
<br /> | <br /> | ||
<u>Solution 1: (salts)</u><br /> | <u>Solution 1: (salts)</u><br /> |
Revision as of 12:17, 18 September 2015
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Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Enzymatic Assay of NB-EsteraseFigure 8: Gel for verification of ligation. There is no band for the ligation sample. Gibson Assembly
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