Difference between revisions of "Team:UiOslo Norway/Experiments/Protein Expression"

 
Line 29: Line 29:
 
<!---------------sponsors------------------------->
 
<!---------------sponsors------------------------->
  
−
<p></br></p>
+
 
−
<div style="float:left; width:100%">
+
 
−
<center><h1>iGEM UiOslo 2015 was sponsored by:</h1>
+
<center><h1>iGEM UiOslo 2015 is sponsored by:</h1>
−
<hr style="height:2px;border:none;color:black; background-color:black;" />
+
<hr style="height:2px;border:none;color:black;background-color:black;" />
 +
<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/e/e0/UiOslo_uio_logo.png" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
Line 38: Line 40:
 
<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
−
<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
 
 
</center>
 
</center>
 
<hr style="height:2px;border:none;color:black;background-color:black;" />
 
<hr style="height:2px;border:none;color:black;background-color:black;" />
−
</div>
 
  
 
<!--------end sponsors---------------------------->
 
<!--------end sponsors---------------------------->

Latest revision as of 12:19, 18 September 2015

Protein Expression:

Back to Protocols


  • Inoculate 5 ml preculture and incubate at 37 °C overnight shaking at 220 rpm

  • Measure OD600 and calculate the amount needed to inoculate an expression culture with and OD600 of 0.3

  • Inoculate the expression culture and incubate at 37 °C for 1 hour shaking at 220 rpm

  • Determine OD600

  • Recommended is an OD600 between 0.4-1.0 to induce protein expression

  • Take a 1 ml sample in an uninduced stage

  • Induce protein expression by adding IPTG to a final concentration of 1 mM

  • Incubate the culture for 3 hours at 37 °C shaking at 220 rpm

  • Harvest cells by centrifugation at 8,000 x g for 5 minutes

    • iGEM UiOslo 2015 is sponsored by: