Difference between revisions of "Team:UiOslo Norway/Experiments/SDS-Page"
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Latest revision as of 12:19, 18 September 2015
SDS-Page:
Back to ProtocolsReagent
Stacking Gel: 0.7 ml 30 % Acrylamide (37.5:1) 1.25 ml Stacking gel buffer (1 M Tris pH 6.8) 3 ml Water 50 ul 10 % (w/v) SDS 5 ul TEMED 50 ul APS
Separating gel:
12 % 18 % 2 ml 2 ml Seperating buffer (1.5 M Tris pH 8.8) 3.2 ml 4.8 ml 30 % Acrylamide (37.5:1) 2.7 ml 1.1 ml Water 80 μl 80 μl 10% (w/v) SDS 8 μl 8 μl TEMED 80 μl 80 μl APS Run SDS-Page for 1 hour at 30 mA per gel
Coomassie-staining
Quick Coomassie staining solution: 1 % (w/v) Coomassie Brilliant Blue R250 50 % (v/v) Methanol 10 % (v/v) Acetic acid Coomassie Destaining solution: 40 % (v/v) Methanol 10 % (v/v) Acetic acid
iGEM UiOslo 2015 is sponsored by:
