Difference between revisions of "Team:UiOslo Norway/Experiments/Dialysis"
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Latest revision as of 12:20, 18 September 2015
Dialysis
Back to Protocols
Combine the elution fractions that are pure and containing the protein of interest.
Dialyse against dialysis buffer for 2 hours and change the buffer after 1 hour.
Dialysis buffer 20 mM Tris-HCl pH 8 50 mM NaCl 0.5 mM EDTA 10 mM beta-Mercaptoethanol