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                                    In figure 4 one can see three labelling RNAs each having different modifications respectively having either a C8 Alkyne-dUTP, 5-EdUTP or C8 Alkyne-dCTP incorporated. The Alexa 488 scan indicates that one Labelling RNA + 5-EdUTP was modified with an alkyne and that the CuAAC reaction was successful, too.
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Revision as of 12:52, 18 September 2015

Click Chemistry

Results

In figure 4 one can see three labelling RNAs each having different modifications respectively having either a C8 Alkyne-dUTP, 5-EdUTP or C8 Alkyne-dCTP incorporated. The Alexa 488 scan indicates that one Labelling RNA + 5-EdUTP was modified with an alkyne and that the CuAAC reaction was successful, too. 

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<img src="800px-Heidelberg_CLT_Fig.4_CuAAC.png" style="min-width:85%;"> 

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