Difference between revisions of "Team:ANU-Canberra/labbook"
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Revision as of 12:58, 18 September 2015
Our Lab Book
Blue light source
We have devised a simple method to create your own light source from cheap, readily accessible materials, that can be customised for light intensity and pulse frequency - with minimal prior knowledge of electric circuits or experience with building them.
CRE-Recombinase Assay
We tested the functionality of the light-activated CRY2/CIB1-Cre-recombinase system using a reporter loxP construct.
FRET Tests
As a proof of principle for the expressability and association of the CRY2-CIB1 optogenetic system, we sought to conduct a fluorescence assay based upon the Forster resonance energy transfer (FRET) phenomenon.
Solubility Tests
Even a successfully expressed protein may not function properly - solubility of our product was one indicator of how successful our light-inducible system would be.
Lab Notes
A documentation of experiments we have run from the beginning to the end of our project.