Difference between revisions of "Team:IISER Pune/Notebook"

Revision as of 13:28, 18 September 2015

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Document the dates you worked on your project.

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Chronological notes of what your team is doing.
Brief descriptions of daily important events.
Pictures of your progress.
Mention who participated in what task.

Inspiration

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25^th June 2015

DH5α competent cells were transformed with the following biobricks:

BioBrick	Part	Backbone	Kit plate, Well
BBa_C0080	araC protein coding sequence	pSB1C3	3,11B
BBa_C0012	LacI protein coding sequence	pSB1C3	pSB1C3

The plates were kept at 37°C for 14 hours.

26^th June 2015

Transformation results:

@@ Line 19: / Line 19: @@
 </div>
+<div class=WordSection1>
+<p class=MsoNormal><span style='mso-spacerun:yes'> </span><span
+style='mso-tab-count:1'>               </span>25<sup>th</sup> June 2015</p>
+<p class=MsoNormal>DH5&#945; competent cells were transformed with the
+following <span class=SpellE>biobricks</span>:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>BioBrick</span></p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Part</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Backbone</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Kit plate, Well</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>araC</span> protein coding sequence</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>pSB1C3</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3,11B</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2;mso-yfti-lastrow:yes'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>LacI</span> protein coding sequence</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>pSB1C3</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>pSB1C3</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>The plates were kept at 37°C for 14 hours.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>26<sup>th</sup> June 2015</p>
+<p class=MsoNormal>Transformation results:</p>
 </html>