Difference between revisions of "Team:TCU Taiwan/Composite Part"

Line 83: Line 83:
 
           <td><a  href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td>
 
           <td><a  href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td>
 
           <td>Coding</td>
 
           <td>Coding</td>
−
           <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide (<I>S.lividans chitinase C</I>)</a></td>
+
           <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide (<I>S.lividans</I> chitinase C)</a></td>
 
           <td>93 bp</td>   
 
           <td>93 bp</td>   
 
         </tr>
 
         </tr>

Revision as of 13:47, 18 September 2015

This year TCU_Taiwan iGEM team had built new standard biological parts for iGEM community. Click on the name of the parts for detailed information.

Composite parts table

Favorite Name Type Description Legth
  BBa_K1727007 Composite Promoter+Lac operator+RBS+signal peptide
+Epinecidin-1+Terminater		 216 bp
  BBa_K1727008 Composite Promoter+Lac operator+RBS+Signal peptide
+Signiferin+Terminater		 179 bp


We used T5 promoter, Lac operator, RBS, terminator on pQE 60 vectors. So we can use IPTG induction to get our product.


We used T5 promoter, Lac operator, RBS, terminator on pQE 60 vectors. So we can use IPTG induction to get our product.



             
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