Difference between revisions of "Team:ANU-Canberra/Parts"

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<a href="http://parts.igem.org/Part:BBa_K1750000" class="image featured"><img src="https://static.igem.org/mediawiki/2015/7/78/Efored.jpg" alt="" /></a>
 
<a href="http://parts.igem.org/Part:BBa_K1750000" class="image featured"><img src="https://static.igem.org/mediawiki/2015/7/78/Efored.jpg" alt="" /></a>
 
<header>
 
<header>
<h3>loxP-flanked RFP</h3>
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<h3>Cre-lox recombination reporter/h3>
 
</header>
 
</header>
<p>Red fluorescent protein flanked by loxP sites that are recombined by Cre-recombinase to excise the intervening gene.</p>
+
<p>LoxP-flanked RFP that when excised by Cre-recombinase enables expression of the downstream kanamycin-resistance gene.</p>
 
<footer>
 
<footer>
 
<a href="http://parts.igem.org/Part:BBa_K1750000" class="button alt">Registry page</a>
 
<a href="http://parts.igem.org/Part:BBa_K1750000" class="button alt">Registry page</a>
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<a href="http://parts.igem.org/Part:BBa_K1750001" class="image featured"><img src="https://static.igem.org/mediawiki/2015/0/04/CIB1.png" alt="" /></a>
 
<a href="http://parts.igem.org/Part:BBa_K1750001" class="image featured"><img src="https://static.igem.org/mediawiki/2015/0/04/CIB1.png" alt="" /></a>
 
<header>
 
<header>
<h3>YFP-CIB1 Fusion</h3>
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<h3>CIB1-YFP reporter</h3>
 
</header>
 
</header>
<p>Yellow fluorescent protein fused to the carboxy-terminus of cryptochrome-interacting basic-helix-loop-helix1.</p>
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<p>YFP fused to the C-terminus of CIB1 as a reporter of blue-light induced CRY2 association for FRET analysis.</p>
 
<footer>
 
<footer>
 
<a href="http://parts.igem.org/Part:BBa_K1750001" class="button alt">Registry page</a>
 
<a href="http://parts.igem.org/Part:BBa_K1750001" class="button alt">Registry page</a>

Revision as of 13:53, 18 September 2015

Our Parts

Cre-lox recombination reporter/h3>

LoxP-flanked RFP that when excised by Cre-recombinase enables expression of the downstream kanamycin-resistance gene.

CIB1-YFP reporter

YFP fused to the C-terminus of CIB1 as a reporter of blue-light induced CRY2 association for FRET analysis.