Revision as of 14:02, 18 September 2015

Chew fight

Parts

We use existing biobrick to create new biobricks we needed:

-T7 promoter with RBS (Bba_K525998) and optimized Laccase from T.Thermophilus (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification

-T7 promoter with RBS and optimized Laccase from T.Thermophilus, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion

We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:

-Cytochrome C of S.oneidensis, a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.

-Yeast signal CXXCH from… responsible for the S. oneidensis CytC translocation into the periplasm using TAT system

-Yeast signal CXXCH from… responsible for the Synechocystis sp CytC translocation into the periplasm using TAT system

We designed some linker to facilitate the meeting of the laccase and the cytochrom C

Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC S.oneidensis

Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC P.denitrificans

Rigid and structured linker designed to link (or connect) T.Thermophilus and CytC Synechocystis sp

Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC P.denitrificans

Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC Synechocystis sp

Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC S.oneidensis

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 <li ><a href="/Team:Aix-Marseille/Measurement">Calendar</a></li>
                                          <li ><a href="/Team:Aix-Marseille/Measurement">Results</a></li>
-                                         <li ><a href="/Team:Aix-Marseille/Protocols2">Protocols2</a></li>
+                                         <li ><a href="/Team:Aix-Marseille/Protocols2">Protocols</a></li>
                                          <li ><a href="/Team:Aix-Marseille/Sequencing/data">Sequencing data</a></li>
                                        </ul>