Difference between revisions of "Team:Mingdao/Lab"

Revision as of 14:21, 18 September 2015

Lab

*

We did our work at P1 level bio lab and worked with E. coli DH5alpha and BL21 strains.

We have safety training by a lab manager in the school and followed standard lab regulation. We (dried them completely in the oven at 100°C) before flame test and application. We recommend that the fire retardant proteins should be purified before use.

Safety training was done at iGEM bio lab at Mingdao High School, which follows the regulation of BioSafety of Mingdao High School and Safety Guide for the Lab of Genetic Engineering from Ministry of Science and Technology in Taiwan.

Autoclave

The genetically engineered bacteria with recombinant DNAs were sterilized before discarded.

Laminar Flow

The bacteria were manipulated in the laminar flow cabinet.

Latex Glove & Laboratory Coat

We always wear gloves and coats in the lab.

Fire Extinguisher

Several extinguishers are prepared in experiments with fire.

Emergency Shower & Eyewash

Emergency shower and eyewash are equipped if there’s any necessaries.

We created fire retardant bacteria, any concerns about the application?

We would like to apply our prototype of fire retardant protein/bacteria to paint and textile industries. We didn’t perform any test with purified proteins so far because of time and cost. We recommend the proteins have better to be purified before any application. If you want to use them as paint, you can just dry and kill them completely like what we did in our test. Furthermore, we’re planning to collaborate with the KraigLabs to make GE silkworm to produce fire retardant fibers by putting the SR genes into the host.

Will there be any biological material escape from our lab?

1) We always killed all the materials including bacteria by standard autoclave procedure before letting them leave the lab.

2) The model of wooden house coated with fire retardant bacteria was dried completely in the oven at 100 for over 30 min. All the bacteria are supposed to be killed by the procedure. The fire retardant effectiveness is based on the composition of the contents, and it still works well after killing the bacteria or decomposing the proteins.

Is it dangerous to burn the materials such as cotton, cloth and wooden house in school?

In flame test, we burned our samples in a chemical lab, which has equipment with chemical fume hoods. In addition, there’s fire extinguisher, fire resisting sand, etc. in a regular lab setting.

2~16: An introduction to iGEM

6: How to use pipette

18: Synthetic biology and iGEM

23~25: Biosensor introduction

30: Definition of DNA, gene, and genome

2: Getting to know “Human Practices” and "Multimedia"

7~9: All about “cell”

● Getting to know "Poster" and "Presentation"

16~21: Introduction to biotechs

● Analysis of nucleic acids, gene cloning, analysis of protein, and immunoassay

27~Nov 18: Project discussion

28~Nov 4: Procedure of DNA cloning

Oct 27~18: Project discussion

18~25: PCR and DNA electrophoresis

● An introduction to DNA electrophoresis, PCR(Polymerase Chain Reaction), and

Kary Mullis, the inventor of PCR.

● Applications of PCR and agarose gel electrophoresis.

27: Introduction to biotechs

● Bacterial Transformation, chemical analysis

2: Started to work on the project: Fire Retardant

9: Paper discussion about fire

14: All about soy protein

25: Lab experiment:Bacteria part1

27: Experiment:

Practice 1: Plasmid extraction

Practice 2: run gel (PCR performed previously)

Run gel: samples from colony PCR

28: Lab meeting

8: Lab experiment:Bacteria part2

9: PCR, Plasmid extraction, RE digestion, agarose gel preparation and running

10: Ligation & Transformation

11: Colony PCR

13~17: Gene cloning of casein, CKII alpha, and CKII beta, etc.

21: Lab meeting:project

25: Media discussion

9: Lab meeting:team T-shirt

13: Visiting fire station

15~18: Filming

22: Present the first part of video

25, 26: Bacterial culture and plasmid extraction

27: Discussion:paper about flame retardancy mechanisms of flame retardants containing phosphorus

4: Plasmid extraction

5: T-shirt design

12: E.coli：SR phosphoprotein production

19~21: Making poster

27: Discuss ideas about our Presentation

14: Present the progress of experiment

14: Paper discussion:Flame-retardant Bio-materials

21: Group meeting: T-shirts and Poster design

23: Group meeting: Wiki design

4~26: Human practice:iGEM for Taiwan

15: Prepare for the “Science and Technology Month” campaign

●Not only popularize science and technology knowledge but also seize this

chance and give an introduction of iGEM to the other students, in order

them to know more about iGEM

25~28: “Science and Technology Month” campaign

26:Film: Fire Introduction

2: Lab meeting: Poster, T-shirt & Art Design

4: Lab meeting: iGEM for Taiwan & Human Practice

7: iGEM First Meet-up with NCTU-Formosa & HSNU-TAIPEI

7: iGEM human practice at 中興 university

8: A meeting about gene modified food

9: Lab meeting: the lab and other activities in summer vacation

11: Lab meeting: web design

14: Human practice at National Museum of Natural Science

9~21: Experiment: LB preparation, PCR, Plasmid extraction,DNA

electrophoresis,DNA clean up, and RE digestion

13-24: gene cloning for Fire Retardant BioBricks

16: Fire Test for Fire Retardant Bio-Coating part I

19-23: iGEM Asian Conference

23: Fire Test for Fire Retardant Bio-Coating part II

30: Prof. Dave Westenberg, co-advisor for the Missouri-Rolla iGEM team, visit iGEM Mingdao

10~16: Lab experiment: protein analysis

15: Practicing oral presentation

19: Filming finished

22: Practicing oral presentation

24, 25, 26: Genetic Engineering Camp for MD iGEM 2016

27: iGEM Team Meet-ups in Taipei & Hsinchu with HSNU-TAIPEI, TAS-TAIPEI, NTU-LIHPAO-Taiwan & NCTU-Formosa

28: Visiting DLuB

31: Team banner design

1~13: wiki design

4: Lab meeting

5,6: Sand painting

10: Prepare bacteria for magic show

12,13: Poster design and making

17: Oral presentation rehearsal

24~28: iGEM Jamboree

Detailed Notes

Row Data

o Plasmid extraction

o PCR clean up

o PCR (KOD-plus, 2X PCR MasterMix)

o Anti-rabbit IgG-HRP antibody

o Anti-mouse IgG-AP antibody

o Chromogen

• DAB

• BCIP/TNBT

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