Difference between revisions of "Team:FAFU-CHINA/Modeling"

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mso-hansi-theme-font:minor-latin;
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mso-font-kerning:1.0pt;}
+
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+
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<![endif]--><!--[if gte mso 9]><xml>
+
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<o:shapedefaults v:ext="edit" spidmax="2049"/>
+
−
</xml><![endif]--><!--[if gte mso 9]><xml>
+
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<o:shapelayout v:ext="edit">
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  <o:idmap v:ext="edit" data="1"/>
+
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+
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+
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<body lang=ZH-CN style='tab-interval:21.0pt;text-justify-trim:punctuation'>
+
−
 
+
−
<div class=WordSection1 style='layout-grid:15.6pt'>
+
−
 
+
−
<p class=MsoTitle><a name="OLE_LINK2"></a><a name="OLE_LINK1"><span
+
−
style='mso-bookmark:OLE_LINK2'><span lang=EN-US style='font-size:14.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif";font-weight:normal;mso-bidi-font-weight:
+
−
bold'>Modeling<o:p></o:p></span></span></a></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>In
+
−
FAFU’s project, it is difficult to measure the quantitative data and determine
+
−
the amount of <span class=SpellE>dsRNAs</span> which is fed to the larvae. So
+
−
in the modeling part, we <a name="OLE_LINK6"></a><a name="OLE_LINK5"></a><a
+
−
name="OLE_LINK4"><span style='mso-bookmark:OLE_LINK5'><span style='mso-bookmark:
+
−
OLE_LINK6'>devote</span></span></a>d to establishing an accurate mathematical
+
−
model to simulate the dsRNA expression according to the mechanism of T7
+
−
promoter. After the model is built, we can determine the relationship between
+
−
the concentration of IPTG and the production of <span class=GramE>dsRNA .</span>
+
−
Then we can <span class=GramE>control <span
+
−
style='mso-spacerun:yes'>&nbsp;</span>the</span> amount of <span class=SpellE>dsRNAs</span>
+
−
which if fed to the larvae by controlling the concentration of IPTG easily.<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>We
+
−
know that T7 promoter is a kind of inducible promoter. Hill equation can be
+
−
used to simulate the effect of T7 promoter. In T7 strength model, the
+
−
independent variable is the concentration of IPTG, and the dependent variable
+
−
is the production of dsRNA.<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><b style='mso-bidi-font-weight:normal'><span lang=EN-US
+
−
style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></b></span></span></p>
+
−
 
+
−
<p class=MsoListParagraph style='margin-left:18.0pt;text-indent:-18.0pt;
+
−
mso-char-indent-count:0;mso-list:l0 level1 lfo1'><span style='mso-bookmark:
+
−
OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><![if !supportLists]><b
+
−
style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'><span style='mso-list:Ignore'>1.<span
+
−
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp; </span></span></span></b><![endif]><b
+
−
style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'>The Model Simulating the Change of
+
−
dsRNA with Time in Different Concentration of IPTG<o:p></o:p></span></b></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>By
+
−
observing the pattern of the data, we figure out that the Logistic equation,
+
−
which is often used to simulate the growth of population, can model the trend
+
−
best. Thus, we adapt the formula <span style='position:relative;top:14.0pt;
+
−
mso-text-raise:-14.0pt'><v:shapetype id="_x0000_t75" coordsize="21600,21600"
+
−
o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe" filled="f"
+
−
stroked="f">
+
−
<v:stroke joinstyle="miter"/>
+
−
<v:formulas>
+
−
  <v:f eqn="if lineDrawn pixelLineWidth 0"/>
+
−
  <v:f eqn="sum @0 1 0"/>
+
−
  <v:f eqn="sum 0 0 @1"/>
+
−
  <v:f eqn="prod @2 1 2"/>
+
−
  <v:f eqn="prod @3 21600 pixelWidth"/>
+
−
  <v:f eqn="prod @3 21600 pixelHeight"/>
+
−
  <v:f eqn="sum @0 0 1"/>
+
−
  <v:f eqn="prod @6 1 2"/>
+
−
  <v:f eqn="prod @7 21600 pixelWidth"/>
+
−
  <v:f eqn="sum @8 21600 0"/>
+
−
  <v:f eqn="prod @7 21600 pixelHeight"/>
+
−
  <v:f eqn="sum @10 21600 0"/>
+
−
</v:formulas>
+
−
<v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
+
−
<o:lock v:ext="edit" aspectratio="t"/>
+
−
</v:shapetype><v:shape id="_x0000_i1025" type="#_x0000_t75" style='width:114.75pt;
+
−
height:36pt' o:ole="">
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image001.wmz" o:title=""/>
+
−
</v:shape></span><!--[if gte mso 9]><xml>
+
−
<o:OLEObject Type="Embed" ProgID="Equation.DSMT4" ShapeID="_x0000_i1025"
+
−
  DrawAspect="Content" ObjectID="_1504112933">
+
−
</o:OLEObject>
+
−
</xml><![endif]--><span style='mso-spacerun:yes'>&nbsp;</span>in the following
+
−
fitting, where <span class=GramE><b style='mso-bidi-font-weight:normal'>a</b>
+
−
is</span> the concentration of dsRNA in the steady state.<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>When
+
−
the concentration of IPTG=0.3mmol/L, the result of curve fitting is: <o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>General
+
−
model:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
style='position:relative;top:14.0pt;mso-text-raise:-14.0pt'><v:shape id="_x0000_i1026"
+
−
type="#_x0000_t75" style='width:153pt;height:48pt' o:ole="">
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image002.wmz" o:title=""/>
+
−
</v:shape></span><!--[if gte mso 9]><xml>
+
−
<o:OLEObject Type="Embed" ProgID="Equation.DSMT4" ShapeID="_x0000_i1026"
+
−
  DrawAspect="Content" ObjectID="_1504112934">
+
−
</o:OLEObject>
+
−
</xml><![endif]--><o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Coefficients
+
−
(with 95% confidence bounds):<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>a =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp; </span><span class=GramE>0.3241<span
+
−
style='mso-spacerun:yes'>&nbsp; </span>(</span>0.299, 0.3492)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>b =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp; </span><span class=GramE>35.95<span
+
−
style='mso-spacerun:yes'>&nbsp; </span>(</span>-79.99, 151.9)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>c =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp; </span><span class=GramE>1.829<span
+
−
style='mso-spacerun:yes'>&nbsp; </span>(</span>0.3231, 3.334)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Goodness
+
−
of fit:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>SSE: 0.00333<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>R-square: 0.9676<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>Adjusted R-square: 0.9567<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>RMSE: 0.02356<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif";
+
−
mso-no-proof:yes'><v:shape id="图片_x0020_1" o:spid="_x0000_i1034" type="#_x0000_t75"
+
−
style='width:415.5pt;height:268.5pt;visibility:visible;mso-wrap-style:square'>
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image003.png" o:title="IPTG3"/>
+
−
</v:shape></span></span></span><span style='mso-bookmark:OLE_LINK1'><span
+
−
style='mso-bookmark:OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'><o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span class=GramE><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'>Figure 1.</span></span></span></span><span
+
−
style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
+
−
lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>
+
−
The curve is matched by the formula mentioned above.<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>When
+
−
the concentration of IPTG=0. 4mmol/L, the result of curve fitting is:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>General
+
−
model:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
style='position:relative;top:14.0pt;mso-text-raise:-14.0pt'><v:shape id="_x0000_i1027"
+
−
type="#_x0000_t75" style='width:179.25pt;height:56.25pt' o:ole="">
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image004.wmz" o:title=""/>
+
−
</v:shape></span><!--[if gte mso 9]><xml>
+
−
<o:OLEObject Type="Embed" ProgID="Equation.DSMT4" ShapeID="_x0000_i1027"
+
−
  DrawAspect="Content" ObjectID="_1504112935">
+
−
</o:OLEObject>
+
−
</xml><![endif]--><o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Coefficients
+
−
(with 95% confidence bounds):<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>a =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
class=GramE>0.3474<span style='mso-spacerun:yes'>&nbsp; </span>(</span>0.3261,
+
−
0.3686)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>b =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
class=GramE>46.66<span style='mso-spacerun:yes'>&nbsp; </span>(</span>-63.55,
+
−
156.9)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>c =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
class=GramE>1.828<span style='mso-spacerun:yes'>&nbsp; </span>(</span>0.7647,
+
−
2.892)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Goodness
+
−
of fit:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>SSE: 0.00232<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>R-square: 0.9808<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>Adjusted R-square: 0.9744<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>RMSE: 0.01966<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif";
+
−
mso-no-proof:yes'><v:shape id="图片_x0020_2" o:spid="_x0000_i1033" type="#_x0000_t75"
+
−
style='width:415.5pt;height:264pt;visibility:visible;mso-wrap-style:square'>
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image005.png" o:title="IPTG4"/>
+
−
</v:shape></span></span></span><span style='mso-bookmark:OLE_LINK1'><span
+
−
style='mso-bookmark:OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'><o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span class=GramE><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'>Figure 2.</span></span></span></span><span
+
−
style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
+
−
lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>
+
−
The curve is matched by the formula mentioned above.<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>When
+
−
the concentration of IPTG=0. 5mmol/L, the result of curve fitting is:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>General
+
−
model:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
style='position:relative;top:14.0pt;mso-text-raise:-14.0pt'><v:shape id="_x0000_i1028"
+
−
type="#_x0000_t75" style='width:181.5pt;height:57pt' o:ole="">
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image006.wmz" o:title=""/>
+
−
</v:shape></span><!--[if gte mso 9]><xml>
+
−
<o:OLEObject Type="Embed" ProgID="Equation.DSMT4" ShapeID="_x0000_i1028"
+
−
  DrawAspect="Content" ObjectID="_1504112936">
+
−
</o:OLEObject>
+
−
</xml><![endif]--><o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Coefficients
+
−
(with 95% confidence bounds):<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>a =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
class=GramE>0.3465<span style='mso-spacerun:yes'>&nbsp; </span>(</span>0.3206,
+
−
0.3723)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>b =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
class=GramE>49.63<span style='mso-spacerun:yes'>&nbsp; </span>(</span>-79.79,
+
−
179)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>c =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
class=GramE>1.76<span style='mso-spacerun:yes'>&nbsp; </span>(</span>0.6333,
+
−
2.886)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Goodness
+
−
of fit:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>SSE: 0.003338<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>R-square: 0.9732<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>Adjusted R-square: 0.9642<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>RMSE: 0.02359<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif";
+
−
mso-no-proof:yes'><v:shape id="图片_x0020_3" o:spid="_x0000_i1032" type="#_x0000_t75"
+
−
style='width:415.5pt;height:264pt;visibility:visible;mso-wrap-style:square'>
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image007.png" o:title="IPTG5"/>
+
−
</v:shape></span></span></span><span style='mso-bookmark:OLE_LINK1'><span
+
−
style='mso-bookmark:OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'><o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Figure
+
−
3 <span class=GramE>The</span> curve is matched by the formula mentioned above.<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoListParagraph style='margin-left:18.0pt;text-indent:-18.0pt;
+
−
mso-char-indent-count:0;mso-list:l0 level1 lfo1'><span style='mso-bookmark:
+
−
OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><![if !supportLists]><b
+
−
style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'><span style='mso-list:Ignore'>2.<span
+
−
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp; </span></span></span></b><![endif]><b
+
−
style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'>The Hill Equation<o:p></o:p></span></b></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>By
+
−
the work of first part, it is found that the concentration of dsRNA will become
+
−
steady after around 4 hours, so we regard the concentration of dsRNA after 4
+
−
hours’ culture as that of steady state. Then the Hill equation is applied to
+
−
model the relationship between the concentration of IPTG and the production of
+
−
dsRNA, the result of curve fitting is: (where </span></span></span><span
+
−
style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span><!--[if gte msEquation 12]><m:oMath><span
+
−
style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span><m:sSub><m:sSubPr><span
+
−
  style='font-size:12.0pt;mso-ansi-font-size:12.0pt;mso-bidi-font-size:12.0pt;
+
−
  font-family:"Cambria Math","serif";mso-ascii-font-family:"Cambria Math";
+
−
  mso-fareast-font-family:"Arial Unicode MS";mso-hansi-font-family:"Arial Unicode MS";
+
−
  mso-bidi-font-family:"Arial Unicode MS"'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><span
+
−
  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><m:r><i
+
−
    style='mso-bidi-font-style:normal'><span lang=EN-US style='font-size:12.0pt;
+
−
    font-family:"Cambria Math","serif";mso-fareast-font-family:"Arial Unicode MS";
+
−
    mso-bidi-font-family:"Arial Unicode MS"'>P</span></i></m:r></span></span><span
+
−
  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span></m:e><m:sub><span
+
−
  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><m:r><i
+
−
    style='mso-bidi-font-style:normal'><span lang=EN-US style='font-size:12.0pt;
+
−
    font-family:"Cambria Math","serif";mso-fareast-font-family:"Arial Unicode MS";
+
−
    mso-bidi-font-family:"Arial Unicode MS"'>max</span></i></m:r></span></span><span
+
−
  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span></m:sub></m:sSub><span
+
−
style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><m:r><i
+
−
  style='mso-bidi-font-style:normal'><span lang=EN-US style='font-size:12.0pt;
+
−
  font-family:"Cambria Math","serif";mso-fareast-font-family:"Arial Unicode MS";
+
−
  mso-hansi-font-family:"Arial Unicode MS";mso-bidi-font-family:"Arial Unicode MS"'>=0.3375</span></i></m:r></span></span></m:oMath><![endif]--><![if !msEquation]><span
+
−
lang=EN-US style='font-size:10.5pt;mso-bidi-font-size:11.0pt;font-family:"Calibri","sans-serif";
+
−
mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
+
−
minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
+
−
mso-bidi-theme-font:minor-bidi;position:relative;top:3.0pt;mso-text-raise:-3.0pt;
+
−
mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;mso-bidi-language:AR-SA'><v:shape
+
−
id="_x0000_i1025" type="#_x0000_t75" style='width:75.75pt;height:15.75pt'>
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image008.png" o:title=""
+
−
  chromakey="white"/>
+
−
</v:shape></span><![endif]><span style='mso-bookmark:OLE_LINK1'><span
+
−
style='mso-bookmark:OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;
+
−
font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;</span>is the maximal data we can get from the
+
−
data)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>General
+
−
model:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
style='position:relative;top:15.0pt;mso-text-raise:-15.0pt'><v:shape id="_x0000_i1029"
+
−
type="#_x0000_t75" style='width:174.75pt;height:57pt' o:ole="">
+
−
<v:imagedata src="collaboration-FAFU-CHINA.files/image009.wmz" o:title=""/>
+
−
</v:shape></span><!--[if gte mso 9]><xml>
+
−
<o:OLEObject Type="Embed" ProgID="Equation.DSMT4" ShapeID="_x0000_i1029"
+
−
  DrawAspect="Content" ObjectID="_1504112938">
+
−
</o:OLEObject>
+
−
</xml><![endif]--><o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Coefficients
+
−
(with 95% confidence bounds):<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>X<sub>M</sub>
+
−
=<span style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
class=GramE>0.1265<span style='mso-spacerun:yes'>&nbsp; </span>(</span>0.08411,
+
−
0.1689)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>n =<span
+
−
style='mso-spacerun:yes'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span
+
−
class=GramE>2.239<span style='mso-spacerun:yes'>&nbsp; </span>(</span>0.8187,
+
−
3.658)<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Goodness
+
−
of fit:<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>SSE: 0.003107<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>R-square: 0.9062<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>Adjusted R-square: 0.8828<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
+
−
OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><span
+
−
style='mso-spacerun:yes'>&nbsp; </span>RMSE: 0.02787<o:p></o:p></span></span></span></p>
+
−
 
+
−
<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
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OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif";
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mso-no-proof:yes'><v:shape id="图片_x0020_4" o:spid="_x0000_i1031" type="#_x0000_t75"
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style='width:415.5pt;height:264pt;visibility:visible;mso-wrap-style:square'>
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<v:imagedata src="collaboration-FAFU-CHINA.files/image010.png" o:title="Hill"/>
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</v:shape></span></span></span><span style='mso-bookmark:OLE_LINK1'><span
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style='mso-bookmark:OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;
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font-family:"Arial Unicode MS","sans-serif"'><o:p></o:p></span></span></span></p>
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<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
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OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Figure
+
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4 <span class=GramE>The</span> curve is matched by the formula mentioned above.<o:p></o:p></span></span></span></p>
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<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
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OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
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<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
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OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>We
+
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sorted out data and parameters and then beautified the graph.<o:p></o:p></span></span></span></p>
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<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
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style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
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  lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Parameter<o:p></o:p></span></span></span></p>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
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  lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>value<o:p></o:p></span></span></span></p>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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  <p class=MsoNormal align=center style='text-align:center'><span
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  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
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  lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>unit<o:p></o:p></span></span></span></p>
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  </td>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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<tr style='mso-yfti-irow:1'>
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  <p class=MsoNormal align=center style='text-align:center'><span
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  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
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  lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>n<o:p></o:p></span></span></span></p>
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  </td>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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  <p class=MsoNormal align=center style='text-align:center'><span
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  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
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  lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>2.239<o:p></o:p></span></span></span></p>
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  </td>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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  lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p>&nbsp;</o:p></span></span></span></p>
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  </td>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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<tr style='mso-yfti-irow:2;mso-yfti-lastrow:yes'>
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  <p class=MsoNormal align=center style='text-align:center'><span
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  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
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  lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>X<sub>M</sub><o:p></o:p></span></span></span></p>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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  <td width=189 valign=top style='width:142.05pt;border-top:none;border-left:
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  <p class=MsoNormal align=center style='text-align:center'><span
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  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
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  lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>0.1265<o:p></o:p></span></span></span></p>
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  </td>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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  <td width=189 valign=top style='width:142.05pt;border-top:none;border-left:
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  style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
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  style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>μ<span
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  lang=EN-US>g/</span>μ<span lang=EN-US>L<o:p></o:p></span></span></span></span></p>
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  </td>
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  <span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'></span></span>
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<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
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OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif";
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mso-no-proof:yes'><v:shape id="图片_x0020_5" o:spid="_x0000_i1030" type="#_x0000_t75"
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style='width:358.5pt;height:268.5pt;visibility:visible;mso-wrap-style:square'>
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<v:imagedata src="collaboration-FAFU-CHINA.files/image011.jpg" o:title=""/>
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</v:shape></span></span></span><span style='mso-bookmark:OLE_LINK1'><span
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style='mso-bookmark:OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;
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font-family:"Arial Unicode MS","sans-serif"'><o:p></o:p></span></span></span></p>
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<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
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OLE_LINK2'><span class=GramE><span lang=EN-US style='font-size:12.0pt;
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font-family:"Arial Unicode MS","sans-serif"'>Figure 5.</span></span></span></span><span
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style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:OLE_LINK2'><span
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lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>
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It shows the relationship between the concentration of IPTG and the production
+
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of dsRNA<o:p></o:p></span></span></span></p>
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<p class=MsoNormal><span style='mso-bookmark:OLE_LINK1'><span style='mso-bookmark:
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OLE_LINK2'><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'>Figure
+
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5 shows the relationship between the concentration of IPTG and the production
+
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of dsRNA. According to this mathematical model, we can work out the accurate
+
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production of dsRNA which is fed to the larvae with the concentration of IPTG
+
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which is put into the bacterial system to induce T7 promoter.</span></span></span><span
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lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS","sans-serif"'><o:p></o:p></span></p>
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<section class="com__section com__section--text com__section--text-img">
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<div class="com__section-half">
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<h1 class="animate slideInLeft">Communication Design</h1>
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<p class="animate slideInLeft delay-2">Communication design is a mixed discipline between design and information-development which is concerned with how media intermission such as printed, crafted, electronic media or presentations communicate with people.</p>
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</div>
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<div class="com__section-half">
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<img class="animate slideInRight delay-3" src="world.svg" alt="" />
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<section class="com__section com__section--text centered">
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<h1 class="animate scaleIn">A5源码</h1>
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+
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</section>
+
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<section class="com__section com__section--text">
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<h1 class="animate slideInLeft ">Communication Design</h1>
+
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<p class="animate slideInLeft delay-3">Communication design is a mixed discipline between design and information-development which is concerned with how media intermission such as printed, crafted, electronic media or presentations communicate with people.</p>
+
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</section>
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<section class="com__section com__section--text com__section--text-img">
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<div class="com__section-half">
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<h1 class="animate slideInLeft">Communication Design</h1>
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<p class="animate slideInLeft delay-2">Communication design is a mixed discipline between design and information-development which is concerned with how media intermission such as printed, crafted, electronic media or presentations communicate with people.</p>
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</div>
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<div class="com__section-half">
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<img class="animate slideInRight delay-3" src="world.svg" alt="" />
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<h1 class="animate scaleIn">Communication Design</h1>
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<img class="animate scaleIn delay-3" src="world.svg" alt="" />
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</section>
+
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<section class="com__section com__section--text">
+
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<h1 class="animate slideInLeft ">Communication Design</h1>
+
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<p class="animate slideInLeft delay-3">Communication design is a mixed discipline between design and information-development which is concerned with how media intermission such as printed, crafted, electronic media or presentations communicate with people.</p>
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</html>
 

Revision as of 14:49, 18 September 2015

Modeling