Difference between revisions of "Team:Tec-Monterrey/Notebook"

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    <div class="notebook-pg">
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<h2>Notebook</h2>
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<p> Document the dates you worked on your project.</p>
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<h5>What should this page have?</h5>
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<ul>
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<li>Chronological notes of what your team is doing.</li>
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<li> Brief descriptions of daily important events.</li>
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<li>Pictures of your progress. </li>
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<li>Mention who participated in what task.</li>
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</ul>
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<h4>Inspiration</h4>
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<p>You can see what others teams have done to organize their notes:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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</ul>
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</div>
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</html>
 
</html>
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{{Tec-Monterrey_Nav}}
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<nav class="submenu">
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              <ul>
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                <li><a href="#">Hello world!</a></li>
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              </ul>
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            </nav>
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        <!-- END NAVIGATION -->
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        </header>
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        <div class="container push-top">
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          <div class="row">
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            <button id="jun-but" class="col-md-3 col-xs-3 cal-but">June</button>
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            <button id="jul-but" class="col-md-3 col-xs-3 cal-but">July</button>
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            <button id="aug-but" class="col-md-3 col-xs-3 cal-but">August</button>
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            <button id="sept-but" class="col-md-3 col-xs-3 cal-but">September</button>
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          </div>
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          <table id="june">
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            <col class="weekend">
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            <col class="weekday" span="5">
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            <col class="weekend">
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            <thead>
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              <tr>
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                <th>Sun</th>
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                <th>Mon</th>
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                <th>Tue</th>
 +
                <th>Wed</th>
 +
                <th>Thu</th>
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                <th>Fri</th>
 +
                <th>Sat</th>
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              </tr>
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            </thead>
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            <tbody>
 +
              <tr>
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                <td></td>
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                <td><div class="day">1</div></td>
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                <td><div class="day">2</div></td>
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                <td><div class="day">3</div></td>
 +
                <td><div class="day">4</div></td>
 +
                <td><div class="day">5</div></td>
 +
                <td><div class="day">6</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><div class="day">7</div></td>
 +
                <td><div class="day">8</div></td>
 +
                <td><div class="day">9</div></td>
 +
                <td><div class="day">10</div></td>
 +
                <td><div class="day">11</div></td>
 +
                <td><div class="day">12</div></td>
 +
                <td><div class="day">13</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><div class="day">14</div></td>
 +
                <td><div class="day">15</div></td>
 +
                <td><div class="day">16</div></td>
 +
                <td><div class="day">17</div></td>
 +
                <td><div class="day">18</div></td>
 +
                <td><div class="day">19</div></td>
 +
                <td><div class="day">20</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><div class="day">21</div></td>
 +
                <td class="workday"><div class="day">22</div>
 +
                    <div class="notes">Miniprep (Dania, Hiyam) Digestion (Carolina) Electrophoresis (David, Alex)</div></td>
 +
                <td class="workday"><div class="day">23</div>
 +
                    <div class="notes">Stock RFP (Dania)</div></td>
 +
                <td><div class="day">24</div></td>
 +
                <td class="workday"><div class="day">25</div>
 +
                    <div class="notes">Miniprep (Hiyam, Dania) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Ausencio, Dania, Hiyam)</div></td>
 +
                <td class="workday"><div class="day">26</div>
 +
                    <div class="notes">Miniprep (Ausencio, Dania, Hiyam) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Karen Carrizales, Dania)</div></td>
 +
                <td class="workday"><div class="day">27</div>
 +
                    <div class="notes">Calcium-competent cells (Jesús)</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">28</div>
 +
                    <div class="notes">Transformation (David, Ernesto)</div></td>
 +
                <td class="workday"><div class="day">29</div>
 +
                    <div class="notes">Inoculations (Alejandro, Carolina, Jesús, Dania)</div></td>
 +
                <td class="workday"><div class="day">30</div>
 +
                    <div class="notes">Miniprep (David, Alejandro) Digestion (Alejandro, Hiyam) Electrophoresis (Alejandro, Ernesto) Digestion (Alejandro, David, Ernesto) Electrophoresis (David, Ernesto)</div></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
              </tr>
 +
            </tbody>
 +
          </table>
 +
          <table id="july">
 +
            <col class="weekend">
 +
            <col class="weekday" span="5">
 +
            <col class="weekend">
 +
            <thead>
 +
              <tr>
 +
                <th>Sun</th>
 +
                <th>Mon</th>
 +
                <th>Tue</th>
 +
                <th>Wed</th>
 +
                <th>Thu</th>
 +
                <th>Fri</th>
 +
                <th>Sat</th>
 +
              </tr>
 +
            </thead>
 +
            <tbody>
 +
              <tr>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td class="workday"><div class="day">1</div>
 +
                    <div class="notes">Purification (David, Minerva, Ernesto) Digestions (Carolina, Karen Carrizales) Electrophoresis (Carolina, Dania, Ausencio) Ligations (Jesús, Alex, Hiyam)</div></td>
 +
                <td class="workday"><div class="day">2</div>
 +
                    <div class="notes">Purification (Carolina, Alejandro) Ligations (Carolina, Alejandro) Transformation (David, Karen Carrizales)</div></td>
 +
                <td class="workday"><div class="day">3</div>
 +
                    <div class="notes">Stock P4 (Hiyam) Miniprep (Hiyam, Carolina)</div></td>
 +
                <td class="workday"><div class="day">4</div>
 +
                    <div class="notes">Digestion (Hiyam, David) Electrophoresis (Carolina) Transformation (David, Jesús) Digestion (Karen Carrizales, Alejandro) Electrophoresis (Alejandro, Karen Carrizales, David) Purification (Hiyam, Dania) Ligations (Hiyam, Alejandro, Dania)</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">5</div>
 +
                    <div class="notes">Transformation (Jesús, Carolina) Inoculations (David, Dania, Karen Carrizales, Alejandro)</div></td>
 +
                <td class="workday"><div class="day">6</div>
 +
                    <div class="notes">Stocks P1, P2, P3, GFP (Carolina, Dania) Miniprep (Carolina, Dania, Ausencio) Inoculations (Jesus, Hiyam)</div></td>
 +
                <td class="workday"><div class="day">7</div>
 +
                    <div class="notes">Degestions (Carolina, Dania, Hiyam) Miniprep (Hiyam, Jesús, David, Ausencio) Digestions (Ausencio, Dania, Hiyam)</div></td>
 +
                <td class="workday"><div class="day">8</div>
 +
                    <div class="notes">Electrophoresis (David, Hiyam, Karen Carrizales, Dania) Purification (Karen Carrizales, Ausencio, David) Electrophoresis (Hiyam, Dania) Ligations (David, Karen Carrizales, Ernesto) Transformation (Minerva)</div></td>
 +
                <td class="workday"><div class="day">9</div>
 +
                    <div class="notes">Electrophoresis (David, Ausencio, Ernesto) Digestion (David, Ernesto) Electrophoresis (David, Carolina, Dania) Purification (Hiyam, Dania) Ligations (David) Miniprep Lowe Budget (Alejandra, Karen Tello)</div></td>
 +
                <td class="workday"><div class="day">10</div>
 +
                    <div class="notes">Digestion (Carolina) Electrophoresis (David, Ausencio) Transformation (David, Ausencio) Purification (Ausencio, David, Dania, Karen Carrizales) Digestion Low Budget (Alejandra, Karen Tello)</div></td>
 +
                <td class="workday"><div class="day">11</div>
 +
                    <div class="notes">Ligations (Carolina)</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">12</div>
 +
                    <div class="notes">Transformation (Carolina, Jesús, Ausencio)</div></td>
 +
                <td class="workday"><div class="day">13</div>
 +
                    <div class="notes">Inoculations (Carolina, David, Karen Carrizales)</div></td>
 +
                <td class="workday"><div class="day">14</div>
 +
                    <div class="notes">Miniprep (Carolina, Ausencio, David) Digestion (Hiyam, David) Transformation (Karen Carrizales, David) Electrophoresis (Hiyam, David)</div></td>
 +
                <td class="workday"><div class="day">15</div>
 +
                    <div class="notes">Inoculations (Karen Carrizales, Jesús)</div></td>
 +
                <td class="workday"><div class="day">16</div>
 +
                    <div class="notes">Miniprep (David, Hiyam) Digestion (David, Hiyam) Electrophoresis (Carolina, Karen Carrizales) Purification (Hiyam, Karen Carrizales, David)</div></td>
 +
                <td class="workday"><div class="day">17</div>
 +
                    <div class="notes">Antibiotic preparation (Hiyam, Carolina) Transformation (Karen Carrizales, David) </div></td>
 +
                <td><div class="day">18</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">19</div>
 +
                    <div class="notes">Miniprep (David) Digestion (Karen Carrizales, Ausencio) Electrophoresis, Purification (Hiyam, David) Inoculations (Ausencio)</div></td>
 +
                <td class="workday"><div class="day">20</div>
 +
                    <div class="notes">Miniprep (David, Hiyam) Miniprep Low Budget, Digestion Lowe Budget (Alejandra, Karen Tello)</div></td>
 +
                <td class="workday"><div class="day">21</div>
 +
                    <div class="notes">Digestion (Hiyam, Alejandra, Claudia) Electrophoresis (Karen Carrizales, Hiyam) Miniprep (David) Digestion (Hiyam, David) Electrophoresis (Hiyam)</div></td>
 +
                <td class="workday"><div class="day">22</div>
 +
                    <div class="notes">Calcium-competent cells (Claudia)</div></td>
 +
                <td><div class="day">23</div></td>
 +
                <td class="workday"><div class="day">24</div>
 +
                    <div class="notes">Miniprep (Hiyam, Dania, Carolina) Calcium-competent cells (Claudia) Transformation, Stocks P1 P2 P3 TOP10 DH5alpha (Alejandro) Digestion (Hiyam, Caro, Dania) Electrophoresis (David, Karen Carrizales)</div></td>
 +
                <td><div class="day">25</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><div class="day">26</div></td>
 +
                <td class="workday"><div class="day">27</div>
 +
                    <div class="notes">Miniprep (Dania, Hiyam, Caro) Digestion, Electrophoresis (David, Alejandro) Digestion (Carolina, Alejandro) Sf9 cells counting and passing (Minerva)</div></td>
 +
                <td><div class="day">28</div></td>
 +
                <td class="workday"><div class="day">29</div>
 +
                    <div class="notes">Digestion (Carolina, David, Hiyam) Electrophoresis (Hiyam, Alejandro) Transformation (Alejandro) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">30</div>
 +
                    <div class="notes">Digestion (Hiyam, Dania, David) Electrophoresis (David, Hiyam, Carolina) Purification (Alejandro, Hiyam) Digestion (Alejandro, Hiyam) Electrophoresis (Hiyam, Alejandro, David)</div></td>
 +
                <td class="workday"><div class="day">31</div>
 +
                    <div class="notes">Electrophoresis (Alejandro, David) Purification (Carolina, David) Digestion (Carolina, Alejandro, Hiyam, Dania) Electrophoresis (David)</div></td>
 +
                <td></td>
 +
              </tr>
 +
            </tbody>
 +
          </table>
 +
          <table id="august">
 +
            <col class="weekend">
 +
            <col class="weekday" span="5">
 +
            <col class="weekend">
 +
            <thead>
 +
              <tr>
 +
                <th>Sun</th>
 +
                <th>Mon</th>
 +
                <th>Tue</th>
 +
                <th>Wed</th>
 +
                <th>Thu</th>
 +
                <th>Fri</th>
 +
                <th>Sat</th>
 +
              </tr>
 +
            </thead>
 +
            <tbody>
 +
              <tr>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td class="workday"><div class="day">1</div>
 +
                    <div class="notes">Purification (Hiyam, Dania) Ligation (Hiyam, Alex, Dania) Inoculations (Dania, Hiyam, Alex, David) Transformation (Carolina, Hiyam, Alejandro) </div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">2</div>
 +
                    <div class="notes">Digestion, Electrophoresis (Alejandro) Calcium-competent cells (Minerva, David) Transformation (Alejandro, Hiyam) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">3</div>
 +
                    <div class="notes">Stocks C2, C4, C5, P4, pFastBac (Alejandro, Hiyam) Miniprep (David, Hiyam) Digestion (Hiyam, David, Dania) Calcium-competent cells (Jesús, Karen Carrizales), Electrophoresis (Carolina, Dania)</div></td>
 +
                <td class="workday"><div class="day">4</div>
 +
                    <div class="notes">Digestion, Electrophoresis (Alejandro, Karen Carrizales, David)</div></td>
 +
                <td class="workday"><div class="day">5</div>
 +
                    <div class="notes">Cell Lysis (Alejandro) Transformation (Hiyam, Alejandro, Karen Tello)</div></td>
 +
                <td class="workday"><div class="day">6</div>
 +
                    <div class="notes">Ligation, Digestion (Hiyam, Alejandro) Miniprep (Hiyam)</div></td>
 +
                <td class="workday"><div class="day">7</div>
 +
                    <div class="notes">Miniprep 1 (David) Miniprep 2 (Alejandro, Karen Tello) Digestion (Karen Tello, Lourdes, Jesús) Electrophoresis (Karen Tello, Lourdes) Sf9 cells counting, passing and criopreservation (Dania, Claudia)</div></td>
 +
                <td><div class="day">8</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><div class="day">9</div></td>
 +
                <td class="workday"><div class="day">10</div>
 +
                    <div class="notes">Electrophoresis (Alejandro) Inoculations (Jesús, David)</div></td>
 +
                <td class="workday"><div class="day">11</div>
 +
                    <div class="notes">Miniprep 1 (Carolina, Karen Carrizales) Miniprep 2 (David) Miniprep 3 (Karen Tello, Alejandro, Hiyam) Digestion (Hiyam) Bacmid extraction (David) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">12</div>
 +
                    <div class="notes">Electrophoresis, Miniprep (Hiyam) Electrophoresis (Karen Tello, Ausencio) Inoculations (Carolina) Transfection (David, Dania, Minerva)</div></td>
 +
                <td class="workday"><div class="day">13</div>
 +
                    <div class="notes">Miniprep (Carolina) Transformation (Alejandro)</div></td>
 +
                <td class="workday"><div class="day">14</div>
 +
                    <div class="notes">SDS-Page (David) Western Blot (Minerva, David)</div></td>
 +
                <td class="workday"><div class="day">15</div>
 +
                    <div class="notes">Luminiscence readings, Biuret Assay (Alejandro) Sf9 cells counting and passing (Dania, Claudia) </div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">16</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia) SDS-Page (David) Western Blot (David, Minerva) Luminiscence readings (Minerva, Alejandro) Virus amplification (Jesús, Karen Carrizales, Claudia)</div></td>
 +
                <td><div class="day">17</div></td>
 +
                <td class="workday"><div class="day">18</div>
 +
                    <div class="notes">Calcium-competents (Alejandro, Hiyam, Ausencio) Transformation, Digestion, Electrophoresis (Hiyam, Karen Tello, Ausencio) Purification, Ligation (Hiyam, Carolina) Inoculations, Miniprep (Carolina, Karen Tello)</div></td>
 +
                <td><div class="day">19</div></td>
 +
                <td class="workday"><div class="day">20</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td><div class="day">21</div></td>
 +
                <td class="workday"><div class="day">22</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">23</div>
 +
                    <div class="notes">Minprep (David) Digestion (Carolina) Bacmid extraction (Karen Carrizales, Carolina) Electrophoresis (Carolina) Inoculations (Karen Tello, Carolina) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">24</div>
 +
                    <div class="notes">Transfection (David, Dania, Minerva)</div></td>
 +
                <td class="workday"><div class="day">25</div>
 +
                    <div class="notes">Miniprep, Digestion, Electrophoresis, Gibson Assembly (Hiyam, Karen Tello, Ausencio) Bradford (Alejandro) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td><div class="day">26</div></td>
 +
                <td class="workday"><div class="day">27</div>
 +
                    <div class="notes">Digestion (Alejandro)</div></td>
 +
                <td class="workday"><div class="day">28</div>
 +
                    <div class="notes">Miniprep, Digestion, Electrophoresis (Hiyam, Alejandro, Ausencio) Purification (Eduardo, Hiyam, Alejandro, Ausencio)</div></td>
 +
                <td class="workday"><div class="day">29</div>
 +
                    <div class="notes">Gibson Assembly (Eduardo, Alejandro, Hiyam, Ausencio) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">30</div>
 +
                    <div class="notes">Viral plaque assay (Jesús, Karen Carrizales, Claudia)</div></td>
 +
                <td class="workday"><div class="day">31</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
                <td></td>
 +
              </tr>
 +
            </tbody>
 +
          </table>
 +
          <table id="september">
 +
            <col class="weekend">
 +
            <col class="weekday" span="5">
 +
            <col class="weekend">
 +
            <thead>
 +
              <tr>
 +
                <th>Sun</th>
 +
                <th>Mon</th>
 +
                <th>Tue</th>
 +
                <th>Wed</th>
 +
                <th>Thu</th>
 +
                <th>Fri</th>
 +
                <th>Sat</th>
 +
              </tr>
 +
            </thead>
 +
            <tbody>
 +
              <tr>
 +
                <td></td>
 +
                <td></td>
 +
                <td class="workday"><div class="day">1</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td><div class="day">2</div></td>
 +
                <td><div class="day">3</div></td>
 +
                <td class="workday"><div class="day">4</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">5</div>
 +
                    <div class="notes">Digestion (Hiyam) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">6</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia) Viral plaque assay (Jesús, Karen Carrizales, Claudia)</div></td>
 +
                <td class="workday"><div class="day">7</div>
 +
                    <div class="notes">Gibson Assembly (Hiyam) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">8</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">9</div>
 +
                    <div class="notes">Gibson Assembly (Eduardo, Hiyam, Alejandro, Ausencio) Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">10</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia) Transfection (David, Dania, Minerva)</div></td>
 +
                <td class="workday"><div class="day">11</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia)</div></td>
 +
                <td class="workday"><div class="day">12</div>
 +
                    <div class="notes">Sf9 cells counting and passing (Dania, Claudia</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td class="workday"><div class="day">13</div>
 +
                    <div class="notes">Viral plaque staining, viral plaque assay (Karen Carrizales, Jesús)</div></td>
 +
                <td><div class="day">14</div></td>
 +
                <td class="workday"><div class="day">15</div>
 +
                    <div class="notes">Digestion (Alejandro, Ausencio) Electrophoresis (David, Eduardo, Ausencio) IMAC chromatography (Karen Carrizales, Jesús, Alejandro) SDS-Page (David) Western Blot (David, Minerva)</div></td>
 +
                <td class="workday"><div class="day">16</div>
 +
                    <div class="notes">Miniprep (Alejandro, Hiyam) SDS-Page, Western Blot (David, Minerva)</div></td>
 +
                <td class="workday"><div class="day">17</div>
 +
                    <div class="notes">Confirmation of parts for submitting (Karen Tello, Ausencio, Hiyam, Alejandro, Eduardo)</div></td>
 +
                <td><div class="day">18</div></td>
 +
                <td><div class="day">19</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><div class="day">20</div></td>
 +
                <td><div class="day">21</div></td>
 +
                <td><div class="day">22</div></td>
 +
                <td><div class="day">23</div></td>
 +
                <td><div class="day">24</div></td>
 +
                <td><div class="day">25</div></td>
 +
                <td><div class="day">26</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><div class="day">27</div></td>
 +
                <td><div class="day">28</div></td>
 +
                <td><div class="day">29</div></td>
 +
                <td><div class="day">30</div></td>
 +
                <td><div class="day">31</div></td>
 +
                <td></td>
 +
                <td></td>
 +
              </tr>
 +
            </tbody>
 +
          </table>
 +
      </div>
 +
      <footer>
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          <div class="container-fluid">
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Revision as of 16:16, 18 September 2015


iGEM MTY 2015

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  • <a href="#">Hello world!</a>
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           <button id="jun-but" class="col-md-3 col-xs-3 cal-but">June</button>
           <button id="jul-but" class="col-md-3 col-xs-3 cal-but">July</button>
           <button id="aug-but" class="col-md-3 col-xs-3 cal-but">August</button>
           <button id="sept-but" class="col-md-3 col-xs-3 cal-but">September</button>
<col class="weekend"> <col class="weekday" span="5"> <col class="weekend"> <thead> </thead> <tbody> </tbody>
Sun Mon Tue Wed Thu Fri Sat
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Miniprep (Dania, Hiyam) Digestion (Carolina) Electrophoresis (David, Alex)
23
Stock RFP (Dania)
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25
Miniprep (Hiyam, Dania) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Ausencio, Dania, Hiyam)
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Miniprep (Ausencio, Dania, Hiyam) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Karen Carrizales, Dania)
27
Calcium-competent cells (Jesús)
28
Transformation (David, Ernesto)
29
Inoculations (Alejandro, Carolina, Jesús, Dania)
30
Miniprep (David, Alejandro) Digestion (Alejandro, Hiyam) Electrophoresis (Alejandro, Ernesto) Digestion (Alejandro, David, Ernesto) Electrophoresis (David, Ernesto)
<col class="weekend"> <col class="weekday" span="5"> <col class="weekend"> <thead> </thead> <tbody> </tbody>
Sun Mon Tue Wed Thu Fri Sat
1
Purification (David, Minerva, Ernesto) Digestions (Carolina, Karen Carrizales) Electrophoresis (Carolina, Dania, Ausencio) Ligations (Jesús, Alex, Hiyam)
2
Purification (Carolina, Alejandro) Ligations (Carolina, Alejandro) Transformation (David, Karen Carrizales)
3
Stock P4 (Hiyam) Miniprep (Hiyam, Carolina)
4
Digestion (Hiyam, David) Electrophoresis (Carolina) Transformation (David, Jesús) Digestion (Karen Carrizales, Alejandro) Electrophoresis (Alejandro, Karen Carrizales, David) Purification (Hiyam, Dania) Ligations (Hiyam, Alejandro, Dania)
5
Transformation (Jesús, Carolina) Inoculations (David, Dania, Karen Carrizales, Alejandro)
6
Stocks P1, P2, P3, GFP (Carolina, Dania) Miniprep (Carolina, Dania, Ausencio) Inoculations (Jesus, Hiyam)
7
Degestions (Carolina, Dania, Hiyam) Miniprep (Hiyam, Jesús, David, Ausencio) Digestions (Ausencio, Dania, Hiyam)
8
Electrophoresis (David, Hiyam, Karen Carrizales, Dania) Purification (Karen Carrizales, Ausencio, David) Electrophoresis (Hiyam, Dania) Ligations (David, Karen Carrizales, Ernesto) Transformation (Minerva)
9
Electrophoresis (David, Ausencio, Ernesto) Digestion (David, Ernesto) Electrophoresis (David, Carolina, Dania) Purification (Hiyam, Dania) Ligations (David) Miniprep Lowe Budget (Alejandra, Karen Tello)
10
Digestion (Carolina) Electrophoresis (David, Ausencio) Transformation (David, Ausencio) Purification (Ausencio, David, Dania, Karen Carrizales) Digestion Low Budget (Alejandra, Karen Tello)
11
Ligations (Carolina)
12
Transformation (Carolina, Jesús, Ausencio)
13
Inoculations (Carolina, David, Karen Carrizales)
14
Miniprep (Carolina, Ausencio, David) Digestion (Hiyam, David) Transformation (Karen Carrizales, David) Electrophoresis (Hiyam, David)
15
Inoculations (Karen Carrizales, Jesús)
16
Miniprep (David, Hiyam) Digestion (David, Hiyam) Electrophoresis (Carolina, Karen Carrizales) Purification (Hiyam, Karen Carrizales, David)
17
Antibiotic preparation (Hiyam, Carolina) Transformation (Karen Carrizales, David)
18
19
Miniprep (David) Digestion (Karen Carrizales, Ausencio) Electrophoresis, Purification (Hiyam, David) Inoculations (Ausencio)
20
Miniprep (David, Hiyam) Miniprep Low Budget, Digestion Lowe Budget (Alejandra, Karen Tello)
21
Digestion (Hiyam, Alejandra, Claudia) Electrophoresis (Karen Carrizales, Hiyam) Miniprep (David) Digestion (Hiyam, David) Electrophoresis (Hiyam)
22
Calcium-competent cells (Claudia)
23
24
Miniprep (Hiyam, Dania, Carolina) Calcium-competent cells (Claudia) Transformation, Stocks P1 P2 P3 TOP10 DH5alpha (Alejandro) Digestion (Hiyam, Caro, Dania) Electrophoresis (David, Karen Carrizales)
25
26
27
Miniprep (Dania, Hiyam, Caro) Digestion, Electrophoresis (David, Alejandro) Digestion (Carolina, Alejandro) Sf9 cells counting and passing (Minerva)
28
29
Digestion (Carolina, David, Hiyam) Electrophoresis (Hiyam, Alejandro) Transformation (Alejandro) Sf9 cells counting and passing (Dania, Claudia)
30
Digestion (Hiyam, Dania, David) Electrophoresis (David, Hiyam, Carolina) Purification (Alejandro, Hiyam) Digestion (Alejandro, Hiyam) Electrophoresis (Hiyam, Alejandro, David)
31
Electrophoresis (Alejandro, David) Purification (Carolina, David) Digestion (Carolina, Alejandro, Hiyam, Dania) Electrophoresis (David)
<col class="weekend"> <col class="weekday" span="5"> <col class="weekend"> <thead> </thead> <tbody> </tbody>
Sun Mon Tue Wed Thu Fri Sat
1
Purification (Hiyam, Dania) Ligation (Hiyam, Alex, Dania) Inoculations (Dania, Hiyam, Alex, David) Transformation (Carolina, Hiyam, Alejandro)
2
Digestion, Electrophoresis (Alejandro) Calcium-competent cells (Minerva, David) Transformation (Alejandro, Hiyam) Sf9 cells counting and passing (Dania, Claudia)
3
Stocks C2, C4, C5, P4, pFastBac (Alejandro, Hiyam) Miniprep (David, Hiyam) Digestion (Hiyam, David, Dania) Calcium-competent cells (Jesús, Karen Carrizales), Electrophoresis (Carolina, Dania)
4
Digestion, Electrophoresis (Alejandro, Karen Carrizales, David)
5
Cell Lysis (Alejandro) Transformation (Hiyam, Alejandro, Karen Tello)
6
Ligation, Digestion (Hiyam, Alejandro) Miniprep (Hiyam)
7
Miniprep 1 (David) Miniprep 2 (Alejandro, Karen Tello) Digestion (Karen Tello, Lourdes, Jesús) Electrophoresis (Karen Tello, Lourdes) Sf9 cells counting, passing and criopreservation (Dania, Claudia)
8
9
10
Electrophoresis (Alejandro) Inoculations (Jesús, David)
11
Miniprep 1 (Carolina, Karen Carrizales) Miniprep 2 (David) Miniprep 3 (Karen Tello, Alejandro, Hiyam) Digestion (Hiyam) Bacmid extraction (David) Sf9 cells counting and passing (Dania, Claudia)
12
Electrophoresis, Miniprep (Hiyam) Electrophoresis (Karen Tello, Ausencio) Inoculations (Carolina) Transfection (David, Dania, Minerva)
13
Miniprep (Carolina) Transformation (Alejandro)
14
SDS-Page (David) Western Blot (Minerva, David)
15
Luminiscence readings, Biuret Assay (Alejandro) Sf9 cells counting and passing (Dania, Claudia)
16
Sf9 cells counting and passing (Dania, Claudia) SDS-Page (David) Western Blot (David, Minerva) Luminiscence readings (Minerva, Alejandro) Virus amplification (Jesús, Karen Carrizales, Claudia)
17
18
Calcium-competents (Alejandro, Hiyam, Ausencio) Transformation, Digestion, Electrophoresis (Hiyam, Karen Tello, Ausencio) Purification, Ligation (Hiyam, Carolina) Inoculations, Miniprep (Carolina, Karen Tello)
19
20
Sf9 cells counting and passing (Dania, Claudia)
21
22
Sf9 cells counting and passing (Dania, Claudia)
23
Minprep (David) Digestion (Carolina) Bacmid extraction (Karen Carrizales, Carolina) Electrophoresis (Carolina) Inoculations (Karen Tello, Carolina) Sf9 cells counting and passing (Dania, Claudia)
24
Transfection (David, Dania, Minerva)
25
Miniprep, Digestion, Electrophoresis, Gibson Assembly (Hiyam, Karen Tello, Ausencio) Bradford (Alejandro) Sf9 cells counting and passing (Dania, Claudia)
26
27
Digestion (Alejandro)
28
Miniprep, Digestion, Electrophoresis (Hiyam, Alejandro, Ausencio) Purification (Eduardo, Hiyam, Alejandro, Ausencio)
29
Gibson Assembly (Eduardo, Alejandro, Hiyam, Ausencio) Sf9 cells counting and passing (Dania, Claudia)
30
Viral plaque assay (Jesús, Karen Carrizales, Claudia)
31
Sf9 cells counting and passing (Dania, Claudia)
<col class="weekend"> <col class="weekday" span="5"> <col class="weekend"> <thead> </thead> <tbody> </tbody>
Sun Mon Tue Wed Thu Fri Sat
1
Sf9 cells counting and passing (Dania, Claudia)
2
3
4
Sf9 cells counting and passing (Dania, Claudia)
5
Digestion (Hiyam) Sf9 cells counting and passing (Dania, Claudia)
6
Sf9 cells counting and passing (Dania, Claudia) Viral plaque assay (Jesús, Karen Carrizales, Claudia)
7
Gibson Assembly (Hiyam) Sf9 cells counting and passing (Dania, Claudia)
8
Sf9 cells counting and passing (Dania, Claudia)
9
Gibson Assembly (Eduardo, Hiyam, Alejandro, Ausencio) Sf9 cells counting and passing (Dania, Claudia)
10
Sf9 cells counting and passing (Dania, Claudia) Transfection (David, Dania, Minerva)
11
Sf9 cells counting and passing (Dania, Claudia)
12
Sf9 cells counting and passing (Dania, Claudia
13
Viral plaque staining, viral plaque assay (Karen Carrizales, Jesús)
14
15
Digestion (Alejandro, Ausencio) Electrophoresis (David, Eduardo, Ausencio) IMAC chromatography (Karen Carrizales, Jesús, Alejandro) SDS-Page (David) Western Blot (David, Minerva)
16
Miniprep (Alejandro, Hiyam) SDS-Page, Western Blot (David, Minerva)
17
Confirmation of parts for submitting (Karen Tello, Ausencio, Hiyam, Alejandro, Eduardo)
18
19
20
21
22
23
24
25
26
27
28
29
30
31
 
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