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       <th class="noBottom"><h3>Results</h3></td>
 
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       <td class="noTop">Lactobacillus plantarum, part of natural microbiota of idli fermentation, was chosen to apply metabolic engineering for production of Vitamin B2.  
 
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Revision as of 16:33, 18 September 2015

Background

Aims

Results

Lactobacillus plantarum, part of natural microbiota of idli fermentation, was chosen to apply metabolic engineering for production of Vitamin B2. Vitamin B2 pathway from Bacillus subtilis was codon optimized for Lactobacillus plantarum and two metabolic bottlenecks were overcame by synthetic promoters to have increased Vitamin B2 production.
  • Successfully cloned whole Vitamin B2 enzymatic pathway in coling strain of E.coli.
  • Successfully cloned RibD, RibT25, RibT48, synthetic promoters P48 into Biobrick backbones and therefore generated four new BioBricks.