Difference between revisions of "Team:William and Mary/Part Collection"

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Revision as of 16:53, 18 September 2015

NOISE - W&M iGEM

Part Collections

In deciding which parts to submit to the iGEM Registry we focused on three main aspects.

First: ensuring our project is as reproducible and extensible as possible. To that end we have submitted all of new composite fluorescent protein parts that we constructed during the project. Second: Making genome integration as straightforward as possible for iGEM teams. In order to accomplish this goal we designed, tested, and validated a new integrator cassette that allows for simple genome integration using either 3A or Gibson Assembly. Third: Increasing the number of tools available for promoter-mediated regulation in synthetic biology. We created and validated an E. coli codon optimized dCas9 variant and a suite of gRNAs to target the most commonly used promoters in iGEM.

Integrator Suites

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Antibiotic Operons

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dCas9s

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gRNAs

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XFPs Under Various Promoters

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G^2

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