Difference between revisions of "Template:Heidelberg/project/standardization/onthesouthern"
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| − | Not only can the HRP be used in the ApatBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligation<x-ref>Kershaw2012</x-ref>. This system can be used to avoid working with radioactive isotopes and also poses an alternative to using fluorescent probes. | + | Not only can the HRP be used in the ApatBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligation<x-ref>Kershaw2012</x-ref> (Fig. 4). This system can be used to avoid working with radioactive isotopes and also poses an alternative to using fluorescent probes. |
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Revision as of 17:02, 18 September 2015
On the Southern Blot
Not only can the HRP be used in the ApatBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligation