|
|
| Line 107: |
Line 107: |
| | , the 5´side of the intron | | , the 5´side of the intron |
| | <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_1332009" > [BBa_1332009]</a> | | <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_1332009" > [BBa_1332009]</a> |
| | + | </p> |
| | + | <p> |
| | + | (2) the 3´side of the intron |
| | + | <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1859015" > [BBa_K1859015]</a> |
| | + | , RBS |
| | + | <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034" > [BBa_B0034]</a> |
| | + | , RFP |
| | + | <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" > [BBa_K1332002]</a> |
| | + | , the 5´side of the intron |
| | + | <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1859016" > [BBa_K1859016]</a> |
| | + | </p> |
| | + | <p> |
| | + | |
| | </p> | | </p> |
| | </font> | | </font> |
Revision as of 17:27, 18 September 2015
|
|
PROJECT
MODELING
Efficiency of circularization of mRNA is proportion to probability of meeting 3’ and 5’ side of the intron which is in the both side of mRNA. In this study, we designed complementary sequences near the 5’side of the intron and the 3’side of the intron to improve the efficiency of circularization of mRNA.
If there are complementary sequences, both splicing sites approach and the reaction of circularization is easy to happen because these sequences make hydrogen bands.
Therefore we predict how much efficiency of circularization improved.
The generators used in this study are (1)
[BBa_K1332011]
(2)
[BBa_K1859026]
(3)
[BBa_K1859024]
(4)
[BBa_K1859025]
mRNA transcribed from these generators are the sequences of the following parts.
(1) the 3´side of the intron
[BBa_K1859008]
, RBS
[BBa_B0034]
, RFP
[BBa_K1332002]
, the 5´side of the intron
[BBa_1332009]
(2) the 3´side of the intron
[BBa_K1859015]
, RBS
[BBa_B0034]
, RFP
[BBa_K1332002]
, the 5´side of the intron
[BBa_K1859016]
|