Difference between revisions of "Team:Warwick/Description"
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<li>Three colonies of each plasmid were then inoculated in LB and grown overnight.</li> | <li>Three colonies of each plasmid were then inoculated in LB and grown overnight.</li> | ||
<li>In the morning, each inoculation was refreshed in M9 + casein amino acid (500x dilution), induced with different concentrations of IPTG (0uM, 250uM, and 500uM IPTG) and grown for another 6 hours.</li> | <li>In the morning, each inoculation was refreshed in M9 + casein amino acid (500x dilution), induced with different concentrations of IPTG (0uM, 250uM, and 500uM IPTG) and grown for another 6 hours.</li> | ||
| − | <li> They were then transferred into a 96 well plate (200uL per well) and | + | <li> They were then transferred into a 96 well plate (200uL per well) and measured their fluorescence every 15 minutes.</li> |
</ul> | </ul> | ||
</p> | </p> | ||
Revision as of 18:04, 18 September 2015