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                                     In figure 4 one can see three labelling RNAs each having different modifications respectively having either a C8 Alkyne-dUTP, 5-EdUTP or C8 Alkyne-dCTP incorporated. The Alexa 488 scan indicates that one Labelling RNA + 5-EdUTP was modified with an alkyne and that the CuAAC reaction was successful, too.  
 
                                     In figure 4 one can see three labelling RNAs each having different modifications respectively having either a C8 Alkyne-dUTP, 5-EdUTP or C8 Alkyne-dCTP incorporated. The Alexa 488 scan indicates that one Labelling RNA + 5-EdUTP was modified with an alkyne and that the CuAAC reaction was successful, too.  
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                                    <img src="https://static.igem.org/mediawiki/2015/thumb/2/23/Heidelberg_CLT_Fig.4_CuAAC.png/800px-Heidelberg_CLT_Fig.4_CuAAC.png" style="min-width:85%;">
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                Figure 4: Enter here
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Revision as of 18:07, 18 September 2015

Click Chemistry

In figure 4 one can see three labelling RNAs each having different modifications respectively having either a C8 Alkyne-dUTP, 5-EdUTP or C8 Alkyne-dCTP incorporated. The Alexa 488 scan indicates that one Labelling RNA + 5-EdUTP was modified with an alkyne and that the CuAAC reaction was successful, too.

Figure 4: Enter here