Revision as of 18:14, 18 September 2015

Lab Equipment

In the lab we used many devices to perform our experiments. Followed is a short description of each equipment.

MiniSpin Centrifuge

The MiniSpin Centrifuge (figure 1A) is a powerful centrifuge that can accelerate up to twelve 1.5/2.0 ml Eppendorf Tubes to a maximum speed of 13,400 rpm (rcf: 12,100 x g). Balance is very important for this equipment. We used this centrifuge for several experiments, such as PCR Purification, Miniprepping, Gel Extraction and preparing samples for FACS.

Figure 1A: MiniSpin Centrifuge

Figure 1B: Tabletop Centrifuge

Tabletop Centrifuge

The Tabletop Centrifuge (figure 1B) can be used to centrifuge culture tubes. For this centrifuge, balance is also very important, just as with the MiniSpin Centrifuge. We used this centrifuge for several experiments, such as miniprepping.

MJ Mini Thermal Cycler

The compact, 48-well MJ Mini thermal cycler (figure 2) is a powerful thermal cycler that has been used to perform all kinds of PCR reaction, such as digestion and ligation. 0.2 ml PCR tubes can be used. Its thermal range is 0-99°C. This thermal cycler also offers a thermal gradient technology so that you can optimize reactions for maximum efficiency and accurate quantitation with a gradient range of 35-99°C.

Figure 2: MJ Mini Thermal Cycler.

ImageQuant 350

This equipment allows high-resolution scans of for example monochromatic fluorescence gels and Western Blots. We used it to make scans of all of our agarose gels and SDS PAGE gels.

Figure 3: ImageQuant 350