Difference between revisions of "Team:Warwick/Results"
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| + | <p>_______________________________________________________________________________________________________________________________________</p> | ||
| + | <h3>Experiment 2: Testing the expression of zinc finger proteins on the surface of E. coli cells upon induction with IPTG </h3> | ||
| + | |||
| + | <p> | ||
| + | Our objective was to prove that the modified protein was being expressed on the surface of the plasmid transformed cells. | ||
| + | </p> | ||
| + | <h5> Zif268 </h5> | ||
| + | <p> | ||
| + | <ul> <li> A: Wild type DH5α-Z1 E. <i>coli</i> cells </li> | ||
| + | <li> B: Uninduced Lpp_OmpA-Zif268 transformed cells</li> | ||
| + | <li> C: Induced Lpp_OmpA-Zif268 transformed cells</li> | ||
| + | </ul> | ||
| + | </p> | ||
| + | <p> | ||
| + | Upon comparison of image B and C,it is clear that the modified protein is being expressed upon induction with IPTG. However, the wild type cell shows fluorescence as well, suggesting that there is an error with our method of washing the cells after the fluorescent antibodies have been applied. The affected cells would fluoresce regardless of surface zinc finger expression. | ||
| + | </p> | ||
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Revision as of 18:25, 18 September 2015
