Difference between revisions of "Team:NJU-China"

Line 165: Line 165:
 
<div class="row">
 
<div class="row">
 
<div class="col-md-12">
 
<div class="col-md-12">
−
<div class="scroll-link-wrapper" style="visibility: visible;margin-top:500px ">
+
<div class="scroll-link-wrapper" style="visibility: visible;margin-top:300px ">
 
<a class="scroll-link" href="#menu"><img src="https://static.igem.org/mediawiki/2015/1/16/NJU-China-file-arrow_down.png">
 
<a class="scroll-link" href="#menu"><img src="https://static.igem.org/mediawiki/2015/1/16/NJU-China-file-arrow_down.png">
 
</a></div>
 
</a></div>
Line 174: Line 174:
 
</div>
 
</div>
  
 +
 +
<B>Opioids and opiates top the list of illicit drugs and cause the most burden of disease and drug-related deaths worldwide. The goal of this project is to develop a strategy to treat opioid addiction. We prepared exosomes (nano-sized vesicles secreted by endogenous cells) from cell factory that was engineered to express a rabies viral glycoprotein peptide (specifically recognize and target neuronal cells) on the exosomal membrane surface. By filling the exosomes with siRNA of the mu opioid receptor (MOR, a primary target for opioids) and injecting the exosomes into mouse bloodstream, we detected efficient passage of the siRNA through blood-brain barrier and specific accumulation of the siRNA in mouse brain. Consequently, siRNA-loaded exosomes significantly reduced MOR mRNA and protein levels in mice. To show the therapeutic potential, siRNA-loaded exosomes strongly restrained morphine-induced conditioned place preference in mice. This project may open up new avenues for future treatment of drug addiction.</B>
 
<!--
 
<!--
 
<img id="home2" src="https://static.igem.org/mediawiki/2015/0/05/NJU-China-home2.png">
 
<img id="home2" src="https://static.igem.org/mediawiki/2015/0/05/NJU-China-home2.png">

Revision as of 19:06, 18 September 2015

展示网页

Opioids and opiates top the list of illicit drugs and cause the most burden of disease and drug-related deaths worldwide. The goal of this project is to develop a strategy to treat opioid addiction. We prepared exosomes (nano-sized vesicles secreted by endogenous cells) from cell factory that was engineered to express a rabies viral glycoprotein peptide (specifically recognize and target neuronal cells) on the exosomal membrane surface. By filling the exosomes with siRNA of the mu opioid receptor (MOR, a primary target for opioids) and injecting the exosomes into mouse bloodstream, we detected efficient passage of the siRNA through blood-brain barrier and specific accumulation of the siRNA in mouse brain. Consequently, siRNA-loaded exosomes significantly reduced MOR mRNA and protein levels in mice. To show the therapeutic potential, siRNA-loaded exosomes strongly restrained morphine-induced conditioned place preference in mice. This project may open up new avenues for future treatment of drug addiction.