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Hijack Module (Fast robust oscillator) Notebook< /a>

Hijack Module (Fast robust oscillator) Notebook< /a>

Hijack Module (Fast robust oscillator) Notebook

25^th June 2015

DH5α competent cells were transformed with the following biobricks:

BioBrick	Part	Backbone	Kit plate, Well
BBa_C0080	araC protein coding sequence	pSB1C3	3,11B
BBa_C0012	LacI protein coding sequence	pSB1C3	pSB1C3

The plates were kept at 37°C for 14 hours.

26^th June 2015

Transformation results:

Biobrick	Number of colonies
BBa_C0080	0
BBa_C0012	3

As number of colonies obtained was very low, the transformation of these biobricks was repeated.

27^th June 2015

Transformation results:

Biobrick	Number of colonies
BBa_C0080	3
BBa_C0012	60

Inoculation:

A colony from BBa_C0080 plate was inoculated in 7ml LB media + 7μL Chloramphenicol. Same was done for BBa_C0012. These cultures were kept at 37°C for 12 hours.

28^th June 2015

No growth was observed in both the liquid cultures.

A new colony was inoculated again.

29^th June 2015

Growth observed in the cultures of BBa_C0080 and BBa_C0012. Gylcerol stocks were made for both.

Plasmid isolation was done using Alkaline Lysis protocol for the 2 cultures. (1.5ml culture x 3)

Concentrations obtained from nanodrop:

Sample	Concentration (ng/μL)	A260/A280
BBa_C0080 (1)	3301.8	2.02
BBa_C0080 (2)	3670.9	1.98
BBa_C0080 (3)	2777.6	1.95
BBa_C0012 (1)	4560.0	1.97
BBa_C0012 (2)	4542.1	1.94
BBa_C0012 (3)	5123.5	1.61

A single digest was set for one of each of these plasmids:

(all volumes in μL)	BBa_C0012	BBa_C0080
PstI	0.3	0.3
10x NEBuffer 3	1	1
100x BSA	0.1	0.1
DNA	0.5	0.5
MilliQ H₂0	8.1	8.1
Total Volume	10	10

- Kept at 37°C for 2 hours

- Heat Inactivation: 80°C for 20 minutes

Gel Electrophoresis:

- Digested samples run on a 1% gel.

- Voltage: 100V

- Time run: 50 minutes

Both samples showed an RNA smear. This occurred as the Alkaline Lysis solution I did not have RNAse. After observing this smear, RNAse was added to the solution.

Re-inoculation of the two cultures was done

1^st July 2015

A plasmid isolation using Alkaline lysis was done.

Sample	Concentration (ng/μL)	A260/A280
BBa_C0080 (1)	4548.1	1.98
BBa_C0080 (2)	3411.9	2.01
BBa_C0080 (3)	3492.6	1.98
BBa_C0012 (1)	2258.4	1.99
BBa_C0012 (2)	2442.6	2.00
BBa_C0012 (3)	2691.4	1.97

A digest was set up with a sample of each plasmid and then run on a gel.

The gel showed bands at correct length, but the band intensity was extremely low and did not reflect the concentration obtained on nanodrop. A new method for plasmid isolation was needed.

2^nd July 2015

BBa_K094120 arrived from iGEM Headquarters and was inoculated in 5ml LB media + 5μL Ampicillin. Kept at 37°C for 12 hours.

3^rd July 2015

The liquid culture of BBa_K094120 was plated and kept overnight at 37°C

5^th July 2015

A single colony was inoculated for BBa_K094120. Kept at 37°C for 12 hours

6^th July 2015

Plasmid isolation of BBa_K094120 using Alkaline Lysis:

Sample	Concentration (ng/μL)	A260/A280
BBa_K094120 (1)	1050.8	1.88
BBa_K094120(2)	1493.7	1.95
BBa_K094120 (3)	1365.2	1.97

29^th July 2015

1% gel run with all samples for the 3 plasmids BBa_C0080, BBa_C0012, BBa_K094120.

All bands seen had low intensity which did not correlate to the concentration on nanodrop.

11^th August 2015

Revive glycerol stocks for BBa_C0080, BBa_K094120 and BBa_C0012. A 50 ml culture was inoculated for BBa_C0080 and BBa_C0012 for a Midiprep using Qiagen kit.

Kept at 37°C overnight

12^th August 2015

Midiprep using Qiagen Kit results for BBa_C0080 and BBa_C0012:

Sample	Concentration (ng/μL)	A260/A280
BBa_C0012	7.0	3.62
BBa_C0080	27.8	2.07

Since results obtained were very low, a new “Qiagen spin miniprep” kit was obtained.

17^th August 2015

Primary cultures used to make an overnight 5ml culture of BBa_C0080, BBa_C0012 and BBa_K094120.

18^th August 2015

Qiagen Spin Miniprep protocol followed. Results:

Sample	Concentration (ng/μL)	A260/A280
BBa_K094120	250.7	1.91
BBa_C0080	165.5	1.92
BBa_C0012	195.4	1.92

A digestion was set to check plasmid length:

(all volumes in μL)	BBa_C0080	BBa_C0012	BBa_K094120
DNA	2	2	2
EcoRI	0.2	0.2	0.2
10x EcoRI Buffer	1	1	1
100x BSA	0.1	0.1	0.1
MilliQ H₂0	6.4	6.4	6.4
Total Volume	10	10	10

Gel: 1% gel run at 100V for 40 minutes.

The bands obtained for all 3 plasmids were at the correct length. The uncut plasmid ran faster than cut plasmid.

21^st August 2015

Inoculated BBa_I13504 (GFP, used as reporter in oscillator construct) from previously transformed plate. Kept at 37°C for 12 hours

22^nd August 2015

- Miniprep of BBa_I13504:

Sample	Concentration (ng/μL)	A260/A280
BBa_I13504	122.4	1.94

- Transformation of the biobrick BBa_B0034 (RBS) in DH5α competent cells (Ampicillin antibiotic)

- Plates kept at 37°C for 14 hours

23^rd August 2015

Inoculated a colony for BBa_B0034 and kept at 37°C for 12 hours.

24^th August 2015

Plasmid Isolation using Qiagen Miniprep kit:

Sample	Concentration (ng/μL)	A260/A280
BBa_B0034	95.2	1.95

25^th August 2015

- Transformation of the biobrick BBa_B0015 (double terminator) in DH5α competent cells (Chloramphenicol antibiotic)

- Plates kept at 37°C for 14 hours

26^th August 2015

Transformation Result:

Number of colonies for BBa_B0015 = 8

- Inoculated BBa_B0015 in 5ml LB media + 5 μL Chloramphenicol

- Kept at 37°C for 12 hours

28^th August 2015

Plasmid Isolation of BBa_B0015 using Qiagen Miniprep kit:

Sample	Concentration (ng/μL)	A260/A280
BBa_B0034	72.3	1.95

29^th August 2015

Single Digest to check plasmid length:

(all volumes in μL)	BBa_B0015	BBa_I13504	BBa_B0034
DNA	4	2	2
EcoRI	0.2	0.2	0.2
10x EcoRI Buffer	1	1	1
100x BSA	0.1	0.1	0.1
MilliQ H₂0	5.7	5.7	5.7
Total Volume	10	10	10

Gel: 1% gel run with uncut and cut samples at 100V for 40 minutes.

The 3 single cut bands were at the right length.

6^th September 2015

Digestion for 3A assembly of constructs of the oscillator construct:

(all volumes given are in μL)	BBa_ K094120	BBa_ C0080	BBa_ C0012	BBa_ I13504	BBa_ B0034	BBa_ B0015	Ampicillin Backbone	Chloramphenicol Backbone	Kanamycin Backbone
DNA	3	3	3	3	4	5	10	10	10
10x NEBuffer 2	2.5	2.5	2.5	2.5	2.5	2.5	2.5	2.5	2.5
BSA	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5
EcoRI	0.5	0.5	0.5	-	-	-	0.5	0.5	0.5
XbaI	-	-	-	0.5	0.5	0.5	-	-	-
SpeI	0.5	0.5	0.5	-	-	-	-	-	-
PstI	-	-	-	0.5	0.5	0.5	0.5	0.5	0.5
H₂0	13	13	13	13	12	11	6	6	6
Total	20	20	20	20	20	20	20	20	20

- Kept at 37°C for 5 hours

- Heat Inactivation at 80°C for 20 minutes

- The following devices were to be assembled:

- BBa_K094120 + BBa_B0034 + Chloramphenicol backbone = Device 2a

- BBa_C0080 + BBa_B0015 + Kanamycin backbone = Device 2b

- BBa_C0012 + + BBa_B0015 + Ampicillin backbone = Device 2c

- BBa_K094120 + BBa_I13504 + Kanamycin backbone = Device 2d

Ligation:

(all volumes in μL)	Device 2a	Device 2b	Device 2c	Device 2d
Ampicillin Backbone	-	-	2	-
Chloramphenicol Backbone	2	-	-	-
Kanamycin Backbone	-	2	-	2
BBa_K094120	2	-	-	2
BBa_B0034	2	-	-	-
BBa_C0080	-	2	-	-
BBa_B0015	-	2	2	-
BBa_C0012	-	-	2	-
BBa_I13504	-	-	-	2
T4 DNA ligase	0.5	0.5	0.5	0.5
Ligase Buffer	1	1	1	1
MilliQ H₂0	2.5	2.5	2.5	2.5
Total Volume	20	20	20	20

Ligation kept overnight at room temperature.

7^th September 2015

- Transformation of the ligation mix into DH5α cells

- Kept at 37°C overnight

8^th September 2015

Transformation results:

Ampicillin Negative Control : Lawn growth

Kanamycin Negative Control : Zero colonies

Plate	Number of Colonies
2a	10
2b	12
2c	Lawn growth
2d	5

Ampicillin degraded. 2c re-transformed

Inoculation of a single colony from each plate in 5ml LB media + 5μL antibiotic

Kept at 37°C for 12 hours

9^th September 2015

Miniprep by Qiagen Kit:

Sample	Concentration (ng/μL)	A260/A280
2a	283.4	1.91
2b	122.3	2.09
2d	226.6	1.96

Single Digest with EcoRI to check plasmid length:

Backbone-Backbone ligation was observed on the gel.

A new ligation was setup. This time insert:vector ratio was increased. Kept at 37°C for 2 hours.

These ligation mix were transformed into Dh5α competent cells.

10^th September 2015

Growth observed in negative control plates.

An overnight digestion was set for the individual plasmids again. Kept at 37°C for 12 hours

11^th September 2015

Ligation set for 2 hours and then transformed.

12^th September 2015

Colonies of device 2a, 2b and 2c obtained. No colonies on 2d.

3 colonies from each plate were inoculated, incubated for 12 hours at 37°C and miniprepped.

These were then digested with EcoRI to check the length of the plasmid on a gel.

The bands were as expected.

An overnight digest followed by 2 hour ligation was set to assemble to following device:

Device	Part1	Part2	Backbone
5a	2a	2b	pSB1A3
5b	2a	2c	pSB1K3

Two samples of 5b were assembled – one low copy and second high copy backbone

BBa_I13504 in Ampicillin backbone was chosen to assemble device 2d. This biobrick was digested and ligated.

Devices 5a, 5b and 2d were transformed.

13^th Spetember 2015

Transformation Results:
Negative Control: Zero

Plate	Number of Colonies
5a	15
5b (low copy)	70
5b (high copy)	26
2d	10

Three colonies were inoculated from each plate, incubated at 37°C and then miniprepped.

These were then single digested using EcorI to check on gel.

2d, 5a and 5b high copy showed correct bands.

18^th September 2015

2d construct was characterized by induction with different concentrations of IPTG and arabinose.

Chromoproteins

8th June, 2015

● The following biobrick parts were revived from the iGEM 2015 distribution plates using the iGEM protocol Standard protocol.

● Transformation was carried with Ultra-competent cells for DH5α strain of E.coli (with 200µL of LB).

● 200µL of the revived product was plated on LA+antibiotic.

● The plates were kept at 37℃ overnight (12-16 hours).

Part Number	Location in Kit	Backbone
J23110 (Constitutive Promoter)	Plate 4, Well 19D	Ampicillin
K592025 (RBS+amilCP)	Plate 1, Well 19C	Chloramphenicol
J04450 (RFP)	Plate 4, Well 2H	Ampicillin

Negative Control: For both antibiotics (Amp and Cam)

Positive Control: RFP

9th June, 2015

Part Number	Number of colonies
J23110 (Promoter)	Many colonies
K592025 (RBS+amilCP)	Many single colonies
J04450 (RFP)	Lawn growth
Negative Control	No colonies

● Next, the colonies were inoculated in 5mL LB+ 0.1% antibiotic.

● They were kept in an incubator at 37℃ overnight (12-16 hours) at around 180-200 rpm.

10th June, 2015

● Growth was observed in K592025, but no growth in J23110.

● Again a colony was picked from the plate with J23110 and inoculated in 5mL LB+ 0.1% antibiotic.

● They were kept in an incubator at 37℃ overnight (12-16 hours) at around 180-200 rpm.

11th June, 2015

● Again no growth was observed in J23110 (even after inoculating twice).

● Transformation was done again for J23110

● Different promoter was also transformed.

Part Number	Location in Kit	Backbone
J23119 (Constitutive Promoter)	Plate 3, Well 17O	Chloramphenicol

13th June, 2015

● The two promoters were tranformed with 200µL SOC media which was prepared using the iGEM protocol (earlier transformations with LB).

● 200µL of the revived product was plated on LA+antibiotic.

● The plates were kept at 37℃ overnight (12-16 hours).

14th June, 2015

Part Number	Number of colonies
J23110 (Promoter 1)	30
K592025 (RBS+amilCP)	3
J23119 (Promoter 2)	47
Negative Control	No colonies

● The colonies obtained were inoculated in 5mL LB+ 0.1% antibiotic.

● They were kept in an incubator at 37℃ overnight (12-16 hours) at around 180-200 rpm.

18th June, 2015

Plasmid isolation was carried out using the Alkaline Lysis Protocol from Sambrook and Maniatis.

Resuspended in TE Buffer.

Results of Nanodrop:

Sample	Vial Number	A260/A280	Concentration (ng/µL)
K592025	1	1.99	6691.9
	2	2.11	4749.5
	3	2.12	4158.3
J23119	1	2.10	4865.3
	2	2.09	2757.4
	3	2.10	4902.3
J23110	1	2.09	5121.8
	2	2.15	8148.0
	3	2.13	3223.8

● The alkaline lysis buffer I wasn’t added with RNase.

● The plasmid isolation was repeated with RNase added to Buffer I.

27th June, 2015

● The colonies were again inoculated in 5mL LB+ 0.1% antibiotic.

● They were kept in an incubator at 37℃ overnight (12-16 hours) at around 180-200 rpm.

28th June, 2015

● RNase (10mg/mL stock) was added to Alkaline Lysis Buffer I.

● Resuspension in milliQ water.

Results of Nanodrop:

Sample	Vial Number	A260/A280	Concentration (ng/µL)
K592025	1	2.13	56.9
	2	1.39	67.4
	3	2.06	58.5
J23119	1	2.16	34.1
	2	2.15	32.3
	3	1.39	116.1
J23110	1	2.08	158.4
	2	2.02	3222.1
	3	1.95	580.4

29th June, 2015

● The colonies were again inoculated in 5mL LB+ 0.1% antibiotic.

● They were kept in an incubator at 37℃ overnight (12-16 hours) at around 180-200 rpm.

30th June-10th July, 2015

Again, alkaline lysis was done.

Results of Nanodrop:

Date	Sample	Vial Number	A260/A280	Concentration (ng/µL)
30/6	J23119	1	1.94	2376.8
30/6	J23119	2	1.95	2301.2
30/6	J23119	3	1.88	1662.9
8/7	J23119	1	1.99	1616.5
8/7	J23110	2	1.98	618.9
10/7	K592025	1	1.96	1641.1
10/7	K592025	2	1.97	1457.0
10/7	K592025	3	1.99	1404.6

26th August and 3rd September

Results for Nanodrop by Qiagen spin mini kit

Sample	A260/A280	Concentration (ng/μL)
J23119	2.04	121.1
J23119	1.89	329.3
K592029	1.96	158
K592029	1.88	407.3

26th August

Single digestion reaction

(in μL)	K592025	J23119
EcoRI	0.3	0.3
DNA	2	2
10XEcoRI Buffer	1	1
100X BSA	0.1	0.1
Distilled water	6.6	6.6
Total Volume	10	10

27th August

3A assembly double digestion

(in μL)	Promoter(J23119)	amilCP(K592025)	Linear backbone
DNA	2	2	10
10X Buffer2	2.5	2.5	2.5
100X BSA	0.5	0.5	0.5
EcoRI	0.5	-	0.5
SpeI	0.5	-	-
PstI	-	0.5	0.5
XbaI	-	0.5	-
DpnI	-	-	0.5
Distlled water	14	14	5.5
Total Volume	20	20	20

Kept at 37°C for 4 hours, heat-kill at 80°C for 20min.

Colonies obtained (~10), were inoculated and miniprep was done with qiagen kit.

Table3: Double digestion for 2A assembly

2A assembly Double digestion:

(in μL)	Promoter(J23119)	amilCP(K592025)
DNA	7.8	7
10X Buffer2	1	1
100X BSA	0.2	0.2
SpeI	0.5	0.5
PstI	0.5	-
XbaI	-	0.5
Distilled water	0	0.8
Total Volume	10	10

@@ Line 3,526: / Line 3,526: @@
 </div>
+<div class=WordSection1>
+<a name="Chromoproteins"></a>
+<p> <h2> Chromoproteins</h2></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>8th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l3 level1 lfo7'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The following <span class=SpellE>biobrick</span> parts
+were revived from the <span class=SpellE><b style='mso-bidi-font-weight:normal'>iGEM</b></span><b
+style='mso-bidi-font-weight:normal'> 2015 distribution plates </b>using the <span
+class=SpellE>iGEM</span> protocol Standard protocol.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l3 level1 lfo7'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>Transformation was carried with Ultra-competent cells
+for <b style='mso-bidi-font-weight:normal'>DH5&#945; </b>strain of <b
+style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'>E.coli
+</i></b>(with 200µL of LB). </p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l3 level1 lfo7'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>200µL of the revived product was plated on <span
+class=SpellE>LA+antibiotic</span>.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l3 level1 lfo7'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The plates were kept at 37&#8451; overnight (12-16
+hours).</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a border=1 cellspacing=0 cellpadding=0 width=624 style='border-collapse:
+ collapse;mso-table-layout-alt:fixed;border:none;mso-border-alt:solid black 1.0pt;
+ mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;mso-border-insideh:
+.0pt solid black;mso-border-insidev:1.0pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Part Number</b></p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Location in Kit</b></p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Backbone</b></p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><span
+  style='font-size:9.5pt;background:white;mso-highlight:white'>J23110
+  (Constitutive Promoter)</span></p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Plate 4,
+  Well 19D</p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Ampicillin</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>K592025 (<span
+  class=SpellE>RBS+amilCP</span>)</p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Plate 1,
+  Well 19C</p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Chloramphenicol</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes'>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J04450
+  (RFP)</p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Plate 4,
+  Well 2H</p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Ampicillin</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>Negative Control</b>:
+For both antibiotics (Amp and Cam)</p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>Positive Control</b>:
+RFP</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>9th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a0 border=1 cellspacing=0 cellpadding=0 width=624
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black 1.0pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:1.0pt solid black;mso-border-insidev:1.0pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Part Number</b></p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Number of colonies</b></p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><span
+  style='font-size:9.5pt;background:white;mso-highlight:white'>J23110
+  (Promoter)</span></p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Many
+  colonies</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>K592025 (<span
+  class=SpellE>RBS+amilCP</span>)</p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Many single
+  colonies</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J04450
+  (RFP)</p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Lawn growth</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4;mso-yfti-lastrow:yes'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Negative
+  Control</p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>No colonies</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l6 level1 lfo1'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>Next, the colonies were inoculated in 5mL LB+ 0.1%
+antibiotic.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l6 level1 lfo1'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>They were kept in an incubator at 37&#8451; overnight
+(12-16 hours) at around 180-200 rpm.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>10th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l0 level1 lfo2'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>Growth was observed in K592025, but no growth in
+J23110.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l0 level1 lfo2'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>Again a colony was picked from the plate with J23110
+and inoculated in 5mL LB+ 0.1% antibiotic.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l0 level1 lfo2'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>They were kept in an incubator at 37&#8451; overnight
+(12-16 hours) at around 180-200 rpm. </p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>11th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l2 level1 lfo3'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>Again no growth was observed in J23110 (even after
+inoculating twice).</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l2 level1 lfo3'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>Transformation was done again for J23110</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l2 level1 lfo3'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>Different promoter was also transformed.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a1 border=1 cellspacing=0 cellpadding=0 width=624
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black 1.0pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:1.0pt solid black;mso-border-insidev:1.0pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Part Number</b></p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Location in Kit</b></p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Backbone</b></p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1;mso-yfti-lastrow:yes'>
+  <td width=208 valign=top style='width:156.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><span
+  style='font-size:9.5pt;background:white;mso-highlight:white'>J23119
+  (Constitutive Promoter)</span></p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Plate 3,
+  Well 17O</p>
+  </td>
+  <td width=208 valign=top style='width:156.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Chloramphenicol</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>13th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l1 level1 lfo4'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The two promoters were <span class=SpellE>tranformed</span>
+with 200µL SOC media which was prepared using the <span class=SpellE>iGEM</span>
+protocol (earlier transformations with LB).</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l1 level1 lfo4'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>200µL of the revived product was plated on <span
+class=SpellE>LA+antibiotic</span>.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l1 level1 lfo4'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The plates were kept at 37&#8451; overnight (12-16
+hours).</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>14th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a2 border=1 cellspacing=0 cellpadding=0 width=624
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black 1.0pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:1.0pt solid black;mso-border-insidev:1.0pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Part Number</b></p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Number of colonies</b></p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><span
+  style='font-size:9.5pt;background:white;mso-highlight:white'>J23110 (Promoter
+)</span></p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>30</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>K592025 (<span
+  class=SpellE>RBS+amilCP</span>)</p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23119
+  (Promoter 2)</p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>47</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4;mso-yfti-lastrow:yes'>
+  <td width=312 valign=top style='width:234.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>Negative
+  Control</p>
+  </td>
+  <td width=312 valign=top style='width:234.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>No colonies</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l6 level1 lfo1'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The colonies obtained were inoculated in 5mL LB+ 0.1%
+antibiotic.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l6 level1 lfo1'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>They were kept in an incubator at 37&#8451; overnight
+(12-16 hours) at around 180-200 rpm.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>18th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Plasmid isolation was carried out using the <b
+style='mso-bidi-font-weight:normal'>Alkaline Lysis Protocol </b>from <span
+class=SpellE>Sambrook</span> and <span class=SpellE>Maniatis</span>.</p>
+<p class=MsoNormal><span class=SpellE><span class=GramE>Resuspended</span></span><span
+class=GramE> in TE Buffer.</span></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>Results of <span
+class=SpellE>Nanodrop</span>:</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a3 border=1 cellspacing=0 cellpadding=0 width=624
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black 1.0pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:1.0pt solid black;mso-border-insidev:1.0pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;height:23.0pt'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt;height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Sample</b></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Vial Number</b></p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>A260/A280</b></p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Concentration (ng/µL)</b></p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>K592025</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1.99</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>6691.9</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.11</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>4749.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.12</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>4158.3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23119</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.10</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>4865.3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.09</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2757.4</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.10</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>4902.3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:7'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23110</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.09</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>5121.8</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:8'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.15</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>8148.0</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:9;mso-yfti-lastrow:yes'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.13</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3223.8</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l5 level1 lfo5'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The alkaline lysis buffer I wasn’t added with RNase. </p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l5 level1 lfo5'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The plasmid isolation was repeated with RNase added to
+Buffer I.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>27th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l6 level1 lfo1'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The colonies were again inoculated in 5mL LB+ 0.1%
+antibiotic.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l6 level1 lfo1'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>They were kept in an incubator at 37&#8451; overnight
+(12-16 hours) at around 180-200 rpm.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>28th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l4 level1 lfo6'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>RNase (10mg/mL stock) was added to Alkaline Lysis
+Buffer I.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l4 level1 lfo6'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>Resuspension in <span class=SpellE>milliQ</span> water.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>Results of <span
+class=SpellE>Nanodrop</span>:</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a4 border=1 cellspacing=0 cellpadding=0 width=624
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black 1.0pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:1.0pt solid black;mso-border-insidev:1.0pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;height:23.0pt'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt;height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Sample</b></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Vial Number</b></p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>A260/A280</b></p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Concentration (ng/µL)</b></p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>K592025</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.13</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>56.9</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1.39</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>67.4</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.06</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>58.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23119</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.16</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>34.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.15</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>32.3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1.39</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>116.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:7'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23110</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.08</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>158.4</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:8'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2.02</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3222.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:9;mso-yfti-lastrow:yes'>
+  <td width=156 valign=top style='width:117.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1.95</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>580.4</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>29th June, 2015</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l6 level1 lfo1'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>The colonies were again inoculated in 5mL LB+ 0.1%
+antibiotic.</p>
+<p class=MsoNormalCxSpMiddle style='margin-left:36.0pt;mso-add-space:auto;
+text-indent:-18.0pt;mso-list:l6 level1 lfo1'><![if !supportLists]><span
+style='mso-list:Ignore'>&#9679;<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span><![endif]>They were kept in an incubator at 37&#8451; overnight
+(12-16 hours) at around 180-200 rpm.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>30th June-10th July,
+</b></p>
+<p class=MsoNormal>Again, alkaline lysis was done.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>Results of <span
+class=SpellE>Nanodrop</span>:</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a5 border=1 cellspacing=0 cellpadding=0 width=624
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black 1.0pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:1.0pt solid black;mso-border-insidev:1.0pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;height:23.0pt'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt;height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Date</b></p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Sample</b></p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Vial Number</b></p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>A260/A280</b></p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:23.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'><b
+  style='mso-bidi-font-weight:normal'>Concentration (ng/µL)</b></p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>30/6</p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border-top:none;border-left:none;
+  border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;mso-border-top-alt:
+  solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23119</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.94</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2376.8</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>30/6</p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border-top:none;border-left:none;
+  border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;mso-border-top-alt:
+  solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23119</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.95</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2301.2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>30/6</p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border-top:none;border-left:none;
+  border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;mso-border-top-alt:
+  solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23119</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.88</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1662.9</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>8/7</p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border-top:none;border-left:none;
+  border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;mso-border-top-alt:
+  solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23119</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.99</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1616.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>8/7</p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border-top:none;border-left:none;
+  border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;mso-border-top-alt:
+  solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>J23110</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.98</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>618.9</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>10/7</p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border-top:none;border-left:none;
+  border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;mso-border-top-alt:
+  solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>K592025</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>1</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.96</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1641.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:7'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>10/7</p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border-top:none;border-left:none;
+  border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;mso-border-top-alt:
+  solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>K592025</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>2</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.97</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1457.0</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:8;mso-yfti-lastrow:yes'>
+  <td width=78 valign=top style='width:58.5pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>10/7</p>
+  </td>
+  <td width=78 valign=top style='width:58.5pt;border-top:none;border-left:none;
+  border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;mso-border-top-alt:
+  solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>K592025</p>
+  </td>
+  <td width=124 valign=top style='width:93.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal;mso-pagination:none'>3</p>
+  </td>
+  <td width=160 valign=top style='width:120.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.99</p>
+  </td>
+  <td width=184 valign=top style='width:138.0pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black 1.0pt;mso-border-left-alt:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1404.6</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><span style='mso-no-proof:yes'><!--[if gte vml 1]><v:shapetype
+ id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t"
+ path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
+ <v:stroke joinstyle="miter"/>
+ <v:formulas>
+  <v:f eqn="if lineDrawn pixelLineWidth 0"/>
+  <v:f eqn="sum @0 1 0"/>
+  <v:f eqn="sum 0 0 @1"/>
+  <v:f eqn="prod @2 1 2"/>
+  <v:f eqn="prod @3 21600 pixelWidth"/>
+  <v:f eqn="prod @3 21600 pixelHeight"/>
+  <v:f eqn="sum @0 0 1"/>
+  <v:f eqn="prod @6 1 2"/>
+  <v:f eqn="prod @7 21600 pixelWidth"/>
+  <v:f eqn="sum @8 21600 0"/>
+  <v:f eqn="prod @7 21600 pixelHeight"/>
+  <v:f eqn="sum @10 21600 0"/>
+ </v:formulas>
+ <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
+ <o:lock v:ext="edit" aspectratio="t"/>
+</v:shapetype><v:shape id="image04.png" o:spid="_x0000_i1027" type="#_x0000_t75"
+ style='width:453.75pt;height:288.75pt;visibility:visible;mso-wrap-style:square'>
+ <v:imagedata src="Chromoproteinsw_files/image001.png" o:title=""/>
+</v:shape><![endif]--><![if !vml]><img width=605 height=385
+src="Chromoproteinsw_files/image002.gif" v:shapes="image04.png"><![endif]></span></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a6 border=0 cellspacing=0 cellpadding=0 width=600
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;mso-yfti-tbllook:
+;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;height:81.0pt'>
+  <td width=576 valign=top style='width:432.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:81.0pt'>
+  <p class=MsoNormal style='mso-pagination:none'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=24 valign=top style='width:18.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;
+  height:81.0pt'>
+  <p class=MsoNormal><o:p>&nbsp;</o:p></p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1;mso-yfti-lastrow:yes'>
+  <td width=576 valign=top style='width:432.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=24 valign=top style='width:18.0pt;border:solid black 1.0pt;
+  mso-border-alt:solid black .75pt;background:white;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal><o:p>&nbsp;</o:p></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>26th August and 3rd
+September</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Results for <span class=SpellE>Nanodrop</span> by <span
+class=SpellE>Qiagen</span> spin mini kit</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a7 border=1 cellspacing=0 cellpadding=0 width=592
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black .75pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:.75pt solid black;mso-border-insidev:.75pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=132 valign=top style='width:99.0pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Sample</p>
+  </td>
+  <td width=175 valign=top style='width:131.25pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>A260/A280</p>
+  </td>
+  <td width=285 valign=top style='width:213.75pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Concentration (ng/<span
+  class=SpellE>&#956;L</span>)</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=132 valign=top style='width:99.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>J23119</p>
+  </td>
+  <td width=175 valign=top style='width:131.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2.04</p>
+  </td>
+  <td width=285 valign=top style='width:213.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>121.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=132 valign=top style='width:99.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>J23119</p>
+  </td>
+  <td width=175 valign=top style='width:131.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.89</p>
+  </td>
+  <td width=285 valign=top style='width:213.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>329.3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=132 valign=top style='width:99.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>K592029</p>
+  </td>
+  <td width=175 valign=top style='width:131.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.96</p>
+  </td>
+  <td width=285 valign=top style='width:213.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>158</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4;mso-yfti-lastrow:yes'>
+  <td width=132 valign=top style='width:99.0pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>K592029</p>
+  </td>
+  <td width=175 valign=top style='width:131.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1.88</p>
+  </td>
+  <td width=285 valign=top style='width:213.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>407.3</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><span style='mso-no-proof:yes'><!--[if gte vml 1]><v:shape
+ id="image05.png" o:spid="_x0000_i1026" type="#_x0000_t75" style='width:468pt;
+ height:282pt;visibility:visible;mso-wrap-style:square'>
+ <v:imagedata src="Chromoproteinsw_files/image003.png" o:title=""/>
+</v:shape><![endif]--><![if !vml]><img width=624 height=376
+src="Chromoproteinsw_files/image004.gif" v:shapes="image05.png"><![endif]></span></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><span style='mso-no-proof:yes'><!--[if gte vml 1]><v:shape
+ id="image02.png" o:spid="_x0000_i1025" type="#_x0000_t75" style='width:468pt;
+ height:279pt;visibility:visible;mso-wrap-style:square'>
+ <v:imagedata src="Chromoproteinsw_files/image005.png" o:title=""/>
+</v:shape><![endif]--><![if !vml]><img width=624 height=372
+src="Chromoproteinsw_files/image006.gif" v:shapes="image02.png"><![endif]></span></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>26th August</b></p>
+<p class=MsoNormal>Single digestion reaction</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=a8 border=1 cellspacing=0 cellpadding=0 width=592
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black .75pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:.75pt solid black;mso-border-insidev:.75pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=199 valign=top style='width:149.25pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>(in <span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=198 valign=top style='width:148.5pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>K592025</p>
+  </td>
+  <td width=195 valign=top style='width:146.25pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>J23119</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=199 valign=top style='width:149.25pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>EcoRI</span></p>
+  </td>
+  <td width=198 valign=top style='width:148.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.3</p>
+  </td>
+  <td width=195 valign=top style='width:146.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=199 valign=top style='width:149.25pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>DNA</p>
+  </td>
+  <td width=198 valign=top style='width:148.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2</p>
+  </td>
+  <td width=195 valign=top style='width:146.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=199 valign=top style='width:149.25pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>10XEcoRI Buffer</p>
+  </td>
+  <td width=198 valign=top style='width:148.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1</p>
+  </td>
+  <td width=195 valign=top style='width:146.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=199 valign=top style='width:149.25pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>100X BSA</p>
+  </td>
+  <td width=198 valign=top style='width:148.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.1</p>
+  </td>
+  <td width=195 valign=top style='width:146.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=199 valign=top style='width:149.25pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Distilled water</p>
+  </td>
+  <td width=198 valign=top style='width:148.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>6.6</p>
+  </td>
+  <td width=195 valign=top style='width:146.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>6.6</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6;mso-yfti-lastrow:yes'>
+  <td width=199 valign=top style='width:149.25pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Total Volume</p>
+  </td>
+  <td width=198 valign=top style='width:148.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>10</p>
+  </td>
+  <td width=195 valign=top style='width:146.25pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>10</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><b style='mso-bidi-font-weight:normal'>27th August</b></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>3A assembly double digestion</p>
+<table class=a9 border=1 cellspacing=0 cellpadding=0 width=624
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black .75pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:.75pt solid black;mso-border-insidev:.75pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>(in <span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Promoter(J23119)</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>amilCP</span>(K592025)</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Linear backbone</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>DNA</p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>10</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>10X Buffer2</p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2.5</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2.5</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>2.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>100X BSA</p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>EcoRI</span></p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>SpeI</span></p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>PstI</span></p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:7'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>XbaI</span></p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:8'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>DpnI</span></p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:9'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>Distlled</span>
+  water</p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>14</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>14</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>5.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:10;mso-yfti-lastrow:yes'>
+  <td width=132 valign=top style='width:98.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Total Volume</p>
+  </td>
+  <td width=175 valign=top style='width:130.95pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>20</p>
+  </td>
+  <td width=167 valign=top style='width:125.2pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>20</p>
+  </td>
+  <td width=151 valign=top style='width:113.05pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>20</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal>Kept at 37°C for 4 hours<span class=GramE>,<span
+style='mso-spacerun:yes'>  </span>heat</span>-kill at 80°C for 20min.</p>
+<p class=MsoNormal>Colonies obtained (~10)<span class=GramE>,<span
+style='mso-spacerun:yes'>  </span>were</span> inoculated and <span
+class=SpellE>miniprep</span> was done with <span class=SpellE>qiagen</span>
+kit.</p>
+<p class=MsoNormal><span style='mso-spacerun:yes'> </span></p>
+<table class=aa border=0 cellspacing=0 cellpadding=0 width=606
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;mso-yfti-tbllook:
+;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'>
+  <td width=606 valign=top style='width:454.75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Table3: Double digestion for 2A
+  assembly</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal>2A assembly Double digestion:</p>
+<p class=MsoNormal><span style='mso-spacerun:yes'> </span></p>
+<table class=ab border=1 cellspacing=0 cellpadding=0 width=592
+ style='border-collapse:collapse;mso-table-layout-alt:fixed;border:none;
+ mso-border-alt:solid black .75pt;mso-yfti-tbllook:1536;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
+ mso-border-insideh:.75pt solid black;mso-border-insidev:.75pt solid black'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>(in <span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Promoter(J23119)</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border:solid black 1.0pt;
+  border-left:none;mso-border-left-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>amilCP</span>(K592025)</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>DNA</p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>7.8</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>7</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>10X Buffer2</p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>100X BSA</p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.2</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>SpeI</span></p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>PstI</span></p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'><span class=SpellE>XbaI</span></p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>-</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:7'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Distilled water</p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>0.8</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:8;mso-yfti-lastrow:yes'>
+  <td width=177 valign=top style='width:132.75pt;border:solid black 1.0pt;
+  border-top:none;mso-border-top-alt:solid black .75pt;padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>Total Volume</p>
+  </td>
+  <td width=209 valign=top style='width:156.75pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>10</p>
+  </td>
+  <td width=206 valign=top style='width:154.5pt;border-top:none;border-left:
+  none;border-bottom:solid black 1.0pt;border-right:solid black 1.0pt;
+  mso-border-top-alt:solid black .75pt;mso-border-left-alt:solid black .75pt;
+  padding:5.0pt 5.0pt 5.0pt 5.0pt'>
+  <p class=MsoNormal style='line-height:normal'>10</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span style='mso-spacerun:yes'> </span></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+</div>
 </body>
 </html>

Difference between revisions of "Team:IISER Pune/Notebook"

Revision as of 19:07, 18 September 2015

List of Note Books

Hijack Module (Fast robust oscillator) Notebook

Chromoproteins