Difference between revisions of "Team:Warwick/Results"
| Line 70: | Line 70: | ||
<ul> <li> A: Wild type DH5α-Z1 E. <i>coli</i> cells </li> | <ul> <li> A: Wild type DH5α-Z1 E. <i>coli</i> cells </li> | ||
<li> B: Uninduced Lpp_OmpA-sZF2 transformed cells</li> | <li> B: Uninduced Lpp_OmpA-sZF2 transformed cells</li> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/0/0c/Warwick_U-2.png" width="300" height="300"> | ||
<li> C: Induced Lpp_OmpA-sZF2 transformed cells</li> | <li> C: Induced Lpp_OmpA-sZF2 transformed cells</li> | ||
<img src="https://static.igem.org/mediawiki/2015/1/13/Warwick_I-2.png" width="300" height="300"> | <img src="https://static.igem.org/mediawiki/2015/1/13/Warwick_I-2.png" width="300" height="300"> | ||
| Line 99: | Line 100: | ||
These induced cells are the most clearly fluorescent from the set that was measured. Based on the previous results we decided to alter the protocol for preparing cells for microscopy, instead of fixing the cells on a glass slide, these cells were in liquid medium on the slide. The alternative protocol can be found here. By comparing images A and C it is clear that the fluorescence is caused by the protein being expressed on the cell surface. Image B shows that the Pl_lac promoter did not leak and cause uninduced cells to express the protein in great enough numbers to be visible. | These induced cells are the most clearly fluorescent from the set that was measured. Based on the previous results we decided to alter the protocol for preparing cells for microscopy, instead of fixing the cells on a glass slide, these cells were in liquid medium on the slide. The alternative protocol can be found here. By comparing images A and C it is clear that the fluorescence is caused by the protein being expressed on the cell surface. Image B shows that the Pl_lac promoter did not leak and cause uninduced cells to express the protein in great enough numbers to be visible. | ||
</p> | </p> | ||
| − | |||
| − | |||
| − | |||
<p>_______________________________________________________________________________________________________________________________________</p> | <p>_______________________________________________________________________________________________________________________________________</p> | ||
| Line 108: | Line 106: | ||
This experiment was conducted with all the zinc fingers, however all but | This experiment was conducted with all the zinc fingers, however all but | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
</html> | </html> | ||
{{WarwickFooter}} | {{WarwickFooter}} | ||
Revision as of 19:09, 18 September 2015
