Difference between revisions of "Team:Chalmers-Gothenburg/Protocols"
| Line 325: | Line 325: | ||
<p><b>PCR:</b> https://2015.igem.org/File:ChalmersgothenburgPCRProtocol.pdf</p> | <p><b>PCR:</b> https://2015.igem.org/File:ChalmersgothenburgPCRProtocol.pdf</p> | ||
| − | <p>PCR Purification</p> | + | <p><b>PCR Purification:</b></p> |
| − | <p>Gel extraction</p> | + | <p><b>Gel extraction:</b></p> |
| − | <p>Gibson Assembly Method</p> | + | <p><b>Gibson Assembly Method:</b></p> |
| − | <p>Sticky-end DNA Insert Ligation into Vector DNA</p> | + | <p><b>Sticky-end DNA Insert Ligation into Vector DNA:</b></p> |
| − | <p>FastDigest Restriction </p> | + | <p><b>FastDigest Restriction:</b></p> |
| − | <p>Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation</p> | + | <p><b>Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation:</b></p> |
| − | <p>Yeast Genomic DNA Extraction LiOAc-SDS</p> | + | <p><b>Yeast Genomic DNA Extraction LiOAc-SDS:</b></p> |
| − | <p>Yeast Transformation by Electroporation</p> | + | <p><b>Yeast Transformation by Electroporation:</b></p> |
| − | <p>Test of Detection System in Saccharomyces cerevisiae</p> | + | <p><b>Test of Detection System in Saccharomyces cerevisiae:</b></p> |
</html> | </html> | ||
Revision as of 19:47, 18 September 2015
Protocols
PCR: https://2015.igem.org/File:ChalmersgothenburgPCRProtocol.pdf
PCR Purification:
Gel extraction:
Gibson Assembly Method:
Sticky-end DNA Insert Ligation into Vector DNA:
FastDigest Restriction:
Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation:
Yeast Genomic DNA Extraction LiOAc-SDS:
Yeast Transformation by Electroporation:
Test of Detection System in Saccharomyces cerevisiae: