Revision as of 19:51, 18 September 2015

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PCR:

PCR Purification:

Gel extraction:

Gibson Assembly Method:

Sticky-end DNA Insert Ligation into Vector DNA:

FastDigest Restriction:

Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation:

Yeast Genomic DNA Extraction LiOAc-SDS:

Yeast Transformation by Electroporation:

Test of Detection System in Saccharomyces cerevisiae:

@@ Line 324: / Line 324: @@
 <p><b>PCR:</b></p>
-https://2015.igem.org/File:ChalmersGothenburgPCRprotocol.pdf
 <p><b>PCR Purification:</b></p>