Difference between revisions of "Team:Aix-Marseille/Parts"
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<p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed: | <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed: | ||
<ul><br/> | <ul><br/> | ||
| − | <li>-T7 promoter with RBS (Bba_K525998) and optimized Laccase from <i>T.Thermophilus</i> (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification </li><br/> | + | <li>-T7 promoter with RBS (Bba_K525998) and optimized Laccase from <i>T.Thermophilus</i> (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)</li><br/> |
| − | <li>-T7 promoter with RBS and optimized Laccase from <i>T.Thermophilus</i>, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion</li> | + | <li>-T7 promoter with RBS and optimized Laccase from <i>T.Thermophilus</i>, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)</li> |
</ul> | </ul> | ||
</div></p></span> | </div></p></span> | ||
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<p class="space20"><div align="justify"><span style ="font-family:Courier New">We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:<br/> | <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:<br/> | ||
<ul> | <ul> | ||
| − | <li>-Cytochrome C of S.oneidensis, a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion. | + | <li>-Cytochrome C <i>Synechocystis sp</i> (BBa_K1601001), a cyanobacteria that suggests a cytochrome c </li><br/> |
| − | + | <li>-Cytochrome C of <i>S.oneidensis</i> (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.</li><br/> | |
| − | <li>-Heme A from E.coli K12 that enables the heme overproduction</li><br/> | + | <li>-Heme A from <i>E.coli</i> K12 (BBa_K1601002)that enables the heme overproduction</li><br/> |
| − | <li>-Yeast signal CXXCH | + | <li>-Yeast signal CXXCH from <i>Synechocystis sp</i> responsible for the <i>S.oneidensis</i> CytC translocation into the periplasm using TAT system (BBa_S05318)</li><br/> |
| − | <li>-Yeast signal CXXCH | + | <li>-Yeast signal CXXCH from <i>Synechocystis sp</i> responsible for the <i>Synechocystis sp</i> CytC translocation into the periplasm using TAT system</li><br/> |
</ul> | </ul> | ||
</div></p></span> | </div></p></span> | ||
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<p class="space20"><div align="justify"><span style ="font-family:Courier New"> | <p class="space20"><div align="justify"><span style ="font-family:Courier New"> | ||
<ul> | <ul> | ||
| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>T.Thermophilus</i> and CytC <i>S.oneidensis</i></li> <br/> | + | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>T.Thermophilus</i> and CytC <i>S.oneidensis (BBa_K1601008)</i></li> <br/> |
| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>T.Thermophilus</i> and CytC <i>P.denitrificans</i></li><br/> | + | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>T.Thermophilus</i> and CytC <i>P.denitrificans (BBa_K1601011)</i></li><br/> |
| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) <i>T.Thermophilus</i> and CytC <i>Synechocystis sp</i></li><br/> | + | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) <i>T.Thermophilus</i> and CytC <i>Synechocystis sp(BBa_K1601013)</i></li><br/> |
| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>P.denitrificans</i></li><br/> | + | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>P.denitrificans (BBa_K1601009)</i></li><br/> |
| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>Synechocystis sp</i></li><br/> | + | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>Synechocystis sp (BBa_K1601010)</i></li><br/> |
| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>S.oneidensis</i></li> | + | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>S.oneidensis(BBa_K1601012)</i></li> |
</ul> </div></p></span> | </ul> </div></p></span> | ||
Revision as of 19:56, 18 September 2015
Parts
New biobricks using existing ones
We use existing biobrick to create new biobricks we needed:
- -T7 promoter with RBS (Bba_K525998) and optimized Laccase from T.Thermophilus (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)
- -T7 promoter with RBS and optimized Laccase from T.Thermophilus, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)
New biobricks we designed
We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:
- -Cytochrome C Synechocystis sp (BBa_K1601001), a cyanobacteria that suggests a cytochrome c
- -Cytochrome C of S.oneidensis (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.
- -Heme A from E.coli K12 (BBa_K1601002)that enables the heme overproduction
- -Yeast signal CXXCH from Synechocystis sp responsible for the S.oneidensis CytC translocation into the periplasm using TAT system (BBa_S05318)
- -Yeast signal CXXCH from Synechocystis sp responsible for the Synechocystis sp CytC translocation into the periplasm using TAT system
Linkers we designed
We designed some linker to facilitate the meeting of the laccase and the cytochrom C
- Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC S.oneidensis (BBa_K1601008)
- Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC P.denitrificans (BBa_K1601011)
- Rigid and structured linker designed to link (or connect) T.Thermophilus and CytC Synechocystis sp(BBa_K1601013)
- Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC P.denitrificans (BBa_K1601009)
- Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC Synechocystis sp (BBa_K1601010)
- Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC S.oneidensis(BBa_K1601012)
