Difference between revisions of "Team:York/Parts"

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  <h1>Some Stuff</h1>
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  <h1>So what do all of these genes do?</h1>
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<p>Template for magic appearing Div's</p>
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<p>BBa_K1807000</p>
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<P>BBa_K1807001</p>
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<P></p>
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<P></p>
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<P></p>
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<P></p>
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<P></p>
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<P></p>
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<P></p>
 
<p>(Click to enlarge images)</p>
 
<p>(Click to enlarge images)</p>
  
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       <ul>
 
       <ul>
 
           <li style="display:block;">Click to view:</li>
 
           <li style="display:block;">Click to view:</li>
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           <li style="display:block;" onclick="toggleSection('PLACEHOLDER1')">Plaeholder no. 1</li>
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           <li style="display:block;" onclick="toggleSection('BBa_K1807000')">BBa_K1807000</li>
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<li style="display:block;" onclick="toggleSection('BBa_K1807001')">BBa_K1807001</li>
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<li style="display:block;" onclick="toggleSection('BBa_K1807002')">BBa_K1807002</li>
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<li style="display:block;" onclick="toggleSection('BBa_K1807003')">BBa_K1807003</li>
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<li style="display:block;" onclick="toggleSection('BBa_K1807004')">BBa_K1807004</li>
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<li style="display:block;" onclick="toggleSection('BBa_K1807005')">BBa_K1807005</li>
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<li style="display:block;" onclick="toggleSection('BBa_K1807006')">BBa_K1807006</li>
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<li style="display:block;" onclick="toggleSection('BBa_K1807007')">BBa_K1807007</li>
 
       </ul>
 
       </ul>
 
   </div>
 
   </div>
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<div class="col-md-9">
 
<div class="col-md-9">
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   <div class="section" id="PLACEHOLDER1">
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   <div class="section" id="BBa_K1807000">
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     <h3>Placeholder no.1</h3>
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     <h3>BBa_K1807000</h3>
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    <ul>
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    <li>This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of Xgal in the medium and chromosomal lacZΔM15 produces blue-coloured colonies. The device was used as an expression vector. SmaI restriction sites flank the lacZ alpha coding sequence. Cutting the part with this enzyme allows for the in-frame insertion of any desired protein coding sequence that contains the splice-in flanking sequences (they can be found in the design section). </li>
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    </ul>
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  </div>
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</div>
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<div class="col-md-9">
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  <div class="section" id="BBa_K1807001">
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    <h3>BBa_K1807001</h3>
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    <ul>
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    <li>This part codes for the exopolyphosphatase (PPX) enzyme of Escherichia coli. PPX is able to release phosphate residues from the ends of a polyphosphate chain.</li>
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    </ul>
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  </div>
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</div>
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 +
<div class="col-md-9">
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  <div class="section" id="BBa_K1807002">
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    <h3>BBa_K1807002</h3>
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    <ul>
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    <li>This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain. </li>
 +
    </ul>
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  </div>
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</div>
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 +
<div class="col-md-9">
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  <div class="section" id="BBa_K1807003">
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    <h3>BBa_K1807003</h3>
 
     <ul>
 
     <ul>
 
     <li>Some info</li>
 
     <li>Some info</li>
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   </div>
 
   </div>
 
</div>
 
</div>
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<div class="col-md-9">
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  <div class="section" id="BBa_K1807004">
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    <h3>BBa_K1807004</h3>
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    <ul>
 +
    <li>Some info</li>
 +
    <li>More info</li>
 +
    </ul>
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  </div>
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</div>
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 +
<div class="col-md-9">
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  <div class="section" id="BBa_K1807005">
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    <h3>BBa_K1807005</h3>
 +
    <ul>
 +
    <li>Some info</li>
 +
    <li>More info</li>
 +
    </ul>
 +
  </div>
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</div>
 +
 +
<div class="col-md-9">
 +
  <div class="section" id="BBa_K1807006">
 +
    <h3>BBa_K1807006</h3>
 +
    <ul>
 +
    <li>Some info</li>
 +
    <li>More info</li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
 +
<div class="col-md-9"
 +
  <div class="section" id="BBa_K1807007">
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    <h3>BBa_K1807007</h3>
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    <ul>
 +
    <li>Some info</li>
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    <li>More info</li>
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    </ul>
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  </div>
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</div>
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</div>
 
</div>

Revision as of 20:08, 18 September 2015

Part Table

Source Organism Gene Part Name Function Characterised Sequenced Submitted
Expression vector “pAdapt” in pSB1C3 lacZalpha BBa_K1807000 Used as cloning vector ✔ ✔ ✔
Escherichia coli EcPPX BBa_K1807001 Phosphate Kinase ✔ ✔
Escherichia coli EcPPK BBa_K1807002 Phosphate Kinase ✔ ✔
Escherichia coli EcPstSCAB Phosphate specific transporter ✔
Kingella oralis KoPPK BBa_K1807006 PolyPhosphate Kinase ✔ ✔
Sinorhizobium meliloti SmPstSCAB Phosphate specific transporter ✔ ✔ ✔
Candidatus Accumulibacter phosphatis ApPPK BA-91 BBa_K1807003 PolyPhosphate kinase ✔ ✔ ✔
Candidatus Accumulibacter phosphatis ApPPK SK-12 BBa_K1807004 PolyPhosphate kinase ✔ ✔ ✔
Candidatus Accumulibacter phosphatis ApPPK UW-1 BBa_K1807005 PolyPhosphate kinase ✔ ✔ ✔
Candidatus Accumulibacter phosphatis ApPstSCAB BBa_K1807007 Phosphate specific transporter ✔ ✔

So what do all of these genes do?

BBa_K1807000

BBa_K1807001

(Click to enlarge images)

  • Click to view:
  • BBa_K1807000
  • BBa_K1807001
  • BBa_K1807002
  • BBa_K1807003
  • BBa_K1807004
  • BBa_K1807005
  • BBa_K1807006
  • BBa_K1807007

BBa_K1807000

  • This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of Xgal in the medium and chromosomal lacZΔM15 produces blue-coloured colonies. The device was used as an expression vector. SmaI restriction sites flank the lacZ alpha coding sequence. Cutting the part with this enzyme allows for the in-frame insertion of any desired protein coding sequence that contains the splice-in flanking sequences (they can be found in the design section).

BBa_K1807001

  • This part codes for the exopolyphosphatase (PPX) enzyme of Escherichia coli. PPX is able to release phosphate residues from the ends of a polyphosphate chain.

BBa_K1807002

  • This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain.

BBa_K1807003

  • Some info
  • More info

BBa_K1807004

  • Some info
  • More info

BBa_K1807005

  • Some info
  • More info

BBa_K1807006

  • Some info
  • More info

BBa_K1807007

  • Some info
  • More info