Difference between revisions of "Team:Tuebingen/Notebook"
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<!-- 29.6. - 5.7. --> | <!-- 29.6. - 5.7. --> | ||
<div id="dia0" class="dia cal"> | <div id="dia0" class="dia cal"> | ||
| − | <h2> | + | <h2>Cre-Dronpa</h2> |
| − | <h2> | + | <p> |
| + | Ligation of gene blocks (Cre, Cre_sn, Dronpa, HCV, HCV+linker, nluc), linker L6, L9, L12, L15, nDegron and NLS with pSB1C3 and transformation in DH10beta: </p> | ||
| + | <p> | ||
| + | pSB-Cre_sn and pSB-Luciferase did not yield correct colonies (transformation repeated), the other constructs resulted in correct colonies | ||
| + | </p> | ||
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <p> Ligation of gene blocks (tADH, loxP WT, loxP66, loxP71) with pSB1C3 and transformation in DH10beta</p> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <p> Mutagenesis primer design pRS313, pRS315, pRS316 </p> | ||
</div> | </div> | ||
| − | |||
<!-- 6.7. - 12.7. --> | <!-- 6.7. - 12.7. --> | ||
<div id="dia1" class="dia cal"> | <div id="dia1" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<div id="dia2" class="dia cal"> | <div id="dia2" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<div id="dia3" class="dia cal"> | <div id="dia3" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<div id="dia4" class="dia cal"> | <div id="dia4" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<!-- 3.8. - 9.8. --> | <!-- 3.8. - 9.8. --> | ||
<div id="dia5" class="dia cal"> | <div id="dia5" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<!-- 10.8. - 16.8. --> | <!-- 10.8. - 16.8. --> | ||
<div id="dia6" class="dia cal"> | <div id="dia6" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<!-- 17.8. - 23.8. --> | <!-- 17.8. - 23.8. --> | ||
<div id="dia7" class="dia cal"> | <div id="dia7" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<!-- 24.8. - 30.8. --> | <!-- 24.8. - 30.8. --> | ||
<div id="dia8" class="dia cal"> | <div id="dia8" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<div id="dia9" class="dia cal"> | <div id="dia9" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<div id="dia10" class="dia cal"> | <div id="dia10" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
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<div id="dia11" class="dia cal"> | <div id="dia11" class="dia cal"> | ||
| − | + | <h2>Cre-Dronpa</h2> | |
| + | <h2>Cre Reporter Cassette</h2> | ||
| + | <h2>pRS Vectors</h2> | ||
| + | <h2>Sensors/Promotors</h2> | ||
| + | <h2>Interlabstudy</h2> | ||
</div> | </div> | ||
Revision as of 20:15, 18 September 2015
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Cre-Dronpa
Ligation of gene blocks (Cre, Cre_sn, Dronpa, HCV, HCV+linker, nluc), linker L6, L9, L12, L15, nDegron and NLS with pSB1C3 and transformation in DH10beta:
pSB-Cre_sn and pSB-Luciferase did not yield correct colonies (transformation repeated), the other constructs resulted in correct colonies
Cre Reporter Cassette
Ligation of gene blocks (tADH, loxP WT, loxP66, loxP71) with pSB1C3 and transformation in DH10beta
pRS Vectors
Mutagenesis primer design pRS313, pRS315, pRS316