Difference between revisions of "Team:NUDT CHINA/Collaborations"

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{{NUDT_CHINA}}
 
 
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<h2> Collaborations</h2>
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<!--IGEM LOGO-->
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<style type="text/css">
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#logoContainer {
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position: fixed;
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top: 30px;
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right: 30px;
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</style>
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<!--NUDT_Container-->
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<style type="text/css">
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#NUDT_Container
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{
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margin: 30px 180px;
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padding: 30px 0px;
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font-family: 'verdana',sans-serif;
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box-shadow: 0 3px 8px rgba(0,0,0,.1);
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position: relative;
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#NUDT_Container table{
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#NUDT_Container td{
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#NUDT_Container ul
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{
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list-style-position:inside;
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padding: 10px 50px;
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border: 0;
  
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<p>
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}
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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#NUDT_Container li
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</p>
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{
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padding: 0;
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border: 0;
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}
  
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<div class="highlightBox">
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#NUDT_Container p{
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text-decoration: none;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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color: #888887;
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position: relative;
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padding: 10px 50px;
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font-size: 16px;
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}
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#NUDT_Container a {
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position: relative;
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    text-decoration:none;
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    color:#d82545;
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}
  
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<h4> Which other teams can we work with? </h4>
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#NUDT_Container a:hover
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<p>
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{
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the <a hreef="https://2015.igem.org/Judging/Awards#Medals">iGEM team collaboration gold medal criterion</a>.
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text-decoration:underline;
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</p>
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}
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#NUDT_Container h1, h2, h3, h4, h5{
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color: #444;
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margin: 20px 0px;
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padding:4px 8px;
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letter-spacing: -1px;
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position: relative;
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}
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#NUDT_Container h1 {
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font:bold 28px/12px 'verdana',sans-serif;
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border-left: 10px solid #444;
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line-height:28px;
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}
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#NUDT_Container h2 {
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font:bold 20px/6px 'verdana',sans-serif;
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border-left: 30px solid #444;
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line-height:20px;
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}
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#NUDT_Container h3 {
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font:bold 16px/4px 'verdana',sans-serif;
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padding-left: 50px;
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line-height:16px;
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}
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/*CASE*/
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#NUDT_Container ca {
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position: relative;
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display: inline-block;
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margin: 10px 100px;
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    padding: 10px 20px;
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    text-decoration: none;
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background-color: #bcbcbc;
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color: #000000;
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border-radius: 8px 8px;
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outline: none;
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cursor: pointer;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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font-size: 16px;
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}
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#NUDT_Container team {
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position: relative;
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display: inline-block;
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width: auto;
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height: auto;
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margin: 10px 50px;
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    padding: 20px 20px;
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    text-decoration: none;
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color: #000000;
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border-radius: 8px 8px;
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outline: none;
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cursor: pointer;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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font-size: 16px;
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}
  
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<p>
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</style>
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In order to meet the gold medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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<!--DIA_Container-->
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</p>
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<style type="text/css">
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</div>
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#NUDT_Dia_Container
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{
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width: auto;
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margin: 0px 100px;
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}
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</style>
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<!--BLANK_Container-->
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<style type="text/css">
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.BLANK_Container
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{
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width: auto;
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}
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</style>
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<!--TEAM-->
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<style type="text/css">
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.team_Container
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{
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margin: 20px 10px;
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padding: 20px 10px;
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width: auto;
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}
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</style>
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<!--NUDT_Footer-->
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<style type="text/css">
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#NUDT_Footer
 +
{
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clear: both;
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width: auto;
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padding: 30px 0px;
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font-family: 'verdana',sans-serif;
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position: relative;
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}
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#NUDT_Footer p{
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text-decoration: none;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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color: #000000;
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position: relative;
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padding: 10px 50px;
 +
font-size: 16px;
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}
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</style>
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<!--Common-->
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<div id = "myMainContainer">
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<div id = "menu">
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<img src="https://static.igem.org/mediawiki/2015/9/99/Menu.png"/>
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<li><a id = "HOME" href="https://2015.igem.org/Team:NUDT_CHINA"><span>HOME</span></a></li>
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<li><a id = "TEAM" href="https://2015.igem.org/Team:NUDT_CHINA/Team"><span>TEAM</span></a></li>
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 +
<li><a id = "PROJECT" href = "#"><span>PROJECT</span></a></li>
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<li><a id = "Description" href = "https://2015.igem.org/Team:NUDT_CHINA/Description" class = "b"><span>Description</span></a></li>
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<li><a id = "Experiments" href = "https://2015.igem.org/Team:NUDT_CHINA/Experiments" class = "b"><span>Experiments &amp; Protocols</span></a></li>
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<li><a id = "Results" href="https://2015.igem.org/Team:NUDT_CHINA/Results"  class = "b"><span>Results</span></a></li>
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<li><a id = "Design" href="https://2015.igem.org/Team:NUDT_CHINA/Design" class = "b"><span>Design</span></a></li>
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<li><a id = "PARTS" href = "#"><span>PARTS</span></a></li>
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<li><a id = "Team" href = "https://2015.igem.org/Team:NUDT_CHINA/Parts" class = "b"><span>Team Parts</span></a></li>
 +
<li><a id = "Basic" href = "https://2015.igem.org/Team:NUDT_CHINA/Basic_Part" class = "b"><span>Basic Parts</span></a></li>
 +
<li><a id = "Composite" href="https://2015.igem.org/Team:NUDT_CHINA/Composite_Part"  class = "b"><span>Composite Parts</span></a></li>
  
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<p>
 
−
Here are some suggestions for projects you could work on with other teams:
 
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</p>
 
  
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<ul>
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<li><a id = "NOTEBOOK" href="https://2015.igem.org/Team:NUDT_CHINA/Notebook"><span>NOTEBOOK</span></a></li>
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li><a id = "ATTRIBUTIONS" href="https://2015.igem.org/Team:NUDT_CHINA/Attributions"><span>ATTRIBUTIONS</span></a></li>
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<li> Characterize another team's part </li>
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<li><a id = "COLLABORATIONS" href="https://2015.igem.org/Team:NUDT_CHINA/Collaborations"><span>COLLABORATIONS</span></a></li>
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<li> Debug a construct </li>
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<li><a id = "HUMAN" href="https://2015.igem.org/Team:NUDT_CHINA/Practices"><span>HUMAN PRACTICES</span></a></li>  
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<li> Model or simulating another team's system </li>
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<li><a id = "SAFETY" href="https://2015.igem.org/Team:NUDT_CHINA/Safety"><span>SAFETY</span></a></li>  
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<li> Test another team's software</li>
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<li><a id = "MODELING" href="https://2015.igem.org/Team:NUDT_CHINA/Modeling"><span>MODELING</span></a></li>
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<li> Help build and test another team's hardware project</li>
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    </div>
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<li> Mentor a high-school team</li>
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    <div id = "logoContainer">
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</ul>
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    <a href="https://igem.org/Main_Page"><img  id = "iGEM_LOGO" src="https://static.igem.org/mediawiki/2015/e/ec/TEAM_NUDT_CHINA_iGEM_Logo.png" style="width: 130px; height:135px"/></a>
 +
    </div>
 +
<link href="https://2015.igem.org/Team:NEFU_China/cleariGEM?action=raw&ctype=text/css" rel="stylesheet" /> </head>
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</html>
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<html>
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<!--Main Container-->
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<div id = "NUDT_Container" class = "clearStyle">
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<!--subCommon-->
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<div>
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<img src="https://static.igem.org/mediawiki/2015/0/06/Team_NUDT_CHINA_banner.jpg" style = "width: 100%; height: auto"/>
 +
</div>
 +
<h1>COLLABORATION</h1>
 +
<table>
 +
<tr>
 +
<p style = "font-size: 12px; text-align: left; padding: 0px 50px 0px 100px;">TIPS1: The left <B style = "color: #d82545">MENU :)</B> links to other NUDT_CHINA website.</p>
 +
<p style = "font-size: 12px; text-align: left; padding: 0px 50px 0px 100px;">TIPS2: The right <B style = "color: #d82545">NAVIGATE :)</B> posites on content in this page. (Valid when <B style = "color: #d82545">UNFOLDED</B>)</p>
 +
<p style = "font-size: 12px; text-align: left; padding: 0px 50px 0px 100px;">TIPS3: Click <B style = "color: #d82545">SUBTITLE</B> to <B style = "color: #d82545">FOLD/UNFOLD</B> related content. </p>
 +
</tr>
 +
<tr><button id = "slideAll" style = "margin: 0px 50px 0px 100px;"> CLICK TO FOLD/UNFOLD ALL</button>
 +
</tr>
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</table>
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<script type="text/javascript">
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$(document).ready(function(){
 +
$("#slideAll").click(function(){
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$(".BLANK_Container").slideToggle(300);
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});
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});
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</script>
 +
<!--Collaboration with NJU_CHINA-->
 +
<h2 id = "pos1">I.Collaboration with NJU_CHINA<B style = "color: #d82545">(CLICK)</B></h2>
 +
<div class = "BLANK_Container" id = "slide1">
 +
<p>NJU_CHINA helped us by constructing one of our TAL effectors (TALE1) using Golden Gate technique. They also edited the original vector pTAL1 provided by the Kit to add EcoRI, SpeI and PstI cutting cite, thus ensuring the engineered TAL effector can meet the RCF10 bio-brick standard. This new vector was then used when we construct the other two TAL effectors ourselves. We really appreciate their assistance in our experiment.</p>
 +
<p>In return, we performed nanoparticle tracking analysis (NTA) for NJU_CHINA to help them to have a more precise determination of the quantity and size of secreted exosomes.</p>
 +
<p>The use of Nanosight enabled quantification and size determination of the EV, as nanoparticles can be automatically tracked and sized based on Brownian motion and the diffusion coefficient. Because exosomes are more homogenous and generally smaller than most EVs with a diameter size ranging from 40 to 120 nm, the percentage of nanoparticles whose size ranges from 40 to 120 nm could be a good indicator of total amount of exosomes. The shift of peak of size distribution of EV from 170 nm to 120 nm and the significant raise of the peak indicated the increase of relative level of exosomes in secreted EVs after overexpression nSMase2 in HEK293 cells.</p>
 +
<p>The result proved the feasible and effective improvement of manufacturing exosomes by overexpressing nSMase2.</p>
 +
<center>
 +
<Table>
 +
<tr>
 +
<td><img src="https://static.igem.org/mediawiki/2015/a/a9/NUDT_CHINA_ColNJU.png" style="width: 600px; height:auto"></td>
 +
</tr>
 +
<tr><td><p style = "font-size: 12px; text-align:left;">Figure 4. Characterization of secreted exosomes after overexpression of nSMase2 in HEK293 cells. (A and B) Representative screen shots of the NTA videos for EV from HEK293 cells under normal condition or after transfection with nSMase2 plasmid. (C-F) Size and intensity of EV from HEK293 cells under normal condition or after transfection with nSMase2 plasmid. (G) Concentration of different particle sizes of exosomes with (red line) or without (blue line) transfection with nSMase2 plasmid. The peak of size distribution of EV shifted from 170 nm to 120 nm after transfection with nsMase2 plasmid, indicating the increase in quantity of secreted exosomes.</p></td></tr>
 +
</Table>
 +
</center>
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<center>
 +
<table>
 +
<tr>
 +
<td style = "padding: 0px 15px;"><img src="https://static.igem.org/mediawiki/2015/c/c4/NUDT-logo.jpg" style = "width: 300px; height:auto;"></td>
 +
<td style = "padding: 0px 15px;"><img src="https://static.igem.org/mediawiki/2015/3/37/NJU-logo-NUDT-col.jpg" style = "width: 200px; height:auto;;"></td>
 +
</tr>
 +
<tr>
 +
<td>NUDT_CHINA</td>
 +
<td>NJU_China</td>
 +
</tr>
 +
</table>
 +
</center>
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
$("#pos1").click(function(){
 +
$("#slide1").slideToggle(300);
 +
});
 +
});
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</script>
 +
</div>
  
 +
<h2 id = "pos2">II.Collaboration with USTC-software<B style = "color: #d82545">(CLICK)</B></h2>
 +
<div class = "BLANK_Container" id = "slide2">
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<p>This year, we helped USTC-software by sending our part to them, which we submitted last year numbered for BBa_k1532006.</p>
 +
<p>They asked us for help because they needed the plasmid luxR numbered for C0062 in their project, but what they obtained in the kit plate was proved to be wrong by sequencing. As luck would have it, we submitted it as one of our bricks last year, so we sent it to them as soon as possible to ensure their project runs smoothly.</p>
 +
<p>You can goto <a href="https://2015.igem.org/Team:USTC-software/Home" style = "color:#d82545; text-decoration:underline;">USTC-software’s Wiki</a> to affirm our contributions.</p>
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<center>
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<table>
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<tr>
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<td style = "padding: 0px 15px;"><img src="https://static.igem.org/mediawiki/2015/c/c4/NUDT-logo.jpg" style = "width: 300px; height:auto;"></td>
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<td style = "padding: 0px 15px;"><img src="https://static.igem.org/mediawiki/2015/1/16/USTC-logo.jpeg" style = "width: 200px; height:auto;"></td>
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</tr>
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<tr>
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<td>NUDT_CHINA</td>
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<td>USTC-software</td>
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</table>
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<h2 id = "pos3">III.Collaboration with NEFU_China<B style = "color: #d82545">(CLICK)</B></h2>
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<div class = "BLANK_Container" id = "slide3">
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<p id = "pos31"></p>
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<h3>Wet Lab: Sent a plasmid vector to NEFU_China</h3>
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<p>This year, we helped NEFU_China by sending a plasmid vector to them. </p>
 +
<p>We got in touch with NEFU_China in the 2015 CiCC (China iGEMer Committee). When we exchanged ideas with others, they expressed the difficulty they faced at that time that they were lack of a pivotal plasmid vector which is too hard to buy. We were pleased to offer it to them as we have stored it in our plasmid library. We can obtain a huge sense of satisfaction by helping our friends get over the trouble.</p>
 +
<p>They asked us for help because they needed the plasmid luxR numbered for C0062 in their project, but what they obtained in the kit plate was proved to be wrong by sequencing. As luck would have it, we submitted it as one of our bricks last year, so we sent it to them as soon as possible to ensure their project runs smoothly.</p>
 +
<p>You can goto <a href="https://2015.igem.org/Team:NEFU_China/Home" style = "color:#d82545; text-decoration:underline;">NEFU_China’s Wiki</a> to affirm our contributions.</p>
 +
<p></p>
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<p id = "pos32"></p>
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<h3>We used and improved NEFU_China’s “Flight iGEM”</h3>
 +
<p>This  year, we helped NEFU_China to improved their “Flight iGEM” platform which aims to build a friendly website to make the wiki development easier.</p>
 +
<p>We got touch with NEFU_China in the 2015 CiCC (China iGEMer Committee). Then, we participated in the “Flight iGEM” beta test. Below is our contributions to “Flight iGEM”:</p>
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<tr>
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<td><p style = 'padding: 0px 90px'>1. Improve the Human-Computer Interaction (HCI) for the “Flight iGEM” Magic function.</p></td>
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<td><p style = 'padding: 0px 90px'>2. Figure out the BUG in the <B style = "color:#d82545">login bar</B> which is significant because the 2015 wiki new rules forbad the revise of login bar.</p></td>
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</tr>
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<tr>
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<td><p style = 'padding: 0px 90px'>3. Contact with the <B style = "font-style:italic">igem.org </B>to make sure the feasibility of  <B style = "font-style:italic">cleariGEM </B>template.</p></td>
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<td><img src="https://static.igem.org/mediawiki/2015/f/fb/NUDT_CHINA_ColNEFU.png" style = "width: 350px; height:auto;"></td>
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<td>We are the users of "Flight iGEM"</td>
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</tr>
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</table>
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</center>
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<p>You can goto <a href="https://2015.igem.org/Team:NEFU_China/Software" style = "color:#d82545; text-decoration:underline;">NEFU_China’s Software page</a> to affirm our contributions and more details about the “Flight iGEM”.</p>
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<p></p>
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<center>
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<table>
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<tr>
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<td style = "padding: 0px 15px;"><img src="https://static.igem.org/mediawiki/2015/c/c4/NUDT-logo.jpg" style = "width: 300px; height:auto;"></td>
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<td style = "padding: 0px 15px;"><img src="https://static.igem.org/mediawiki/2015/f/ff/NEFU-logo.png" style = "width: 200px; height:auto;;"></td>
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</tr>
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<td>NUDT_CHINA</td>
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<td>NEFU_China</td>
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</table>
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<li><a href="#myMainContainer" class = "t"><span>TOP/TIP</span></a></li>
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<li><a href="#pos1"><span>NJU_CHINA</span></a></li>
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<li><a href="#pos2"><span>USTC-software</span></a></li>
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<li><a href="#pos3"><span>NEFU_China</span></a></li>
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<li><a href="#pos31" class = 'b'><span>Wet Lab</span></a></li>
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<li><a href="#pos32" class = 'b'><span>Wiki Build</span></a></li>
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<img src="https://static.igem.org/mediawiki/2015/9/93/Team_NUDT_CHINA_Navigate.png"/>
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Revision as of 20:16, 18 September 2015

COLLABORATION

TIPS1: The left MENU :) links to other NUDT_CHINA website.

TIPS2: The right NAVIGATE :) posites on content in this page. (Valid when UNFOLDED)

TIPS3: Click SUBTITLE to FOLD/UNFOLD related content.

I.Collaboration with NJU_CHINA(CLICK)

NJU_CHINA helped us by constructing one of our TAL effectors (TALE1) using Golden Gate technique. They also edited the original vector pTAL1 provided by the Kit to add EcoRI, SpeI and PstI cutting cite, thus ensuring the engineered TAL effector can meet the RCF10 bio-brick standard. This new vector was then used when we construct the other two TAL effectors ourselves. We really appreciate their assistance in our experiment.

In return, we performed nanoparticle tracking analysis (NTA) for NJU_CHINA to help them to have a more precise determination of the quantity and size of secreted exosomes.

The use of Nanosight enabled quantification and size determination of the EV, as nanoparticles can be automatically tracked and sized based on Brownian motion and the diffusion coefficient. Because exosomes are more homogenous and generally smaller than most EVs with a diameter size ranging from 40 to 120 nm, the percentage of nanoparticles whose size ranges from 40 to 120 nm could be a good indicator of total amount of exosomes. The shift of peak of size distribution of EV from 170 nm to 120 nm and the significant raise of the peak indicated the increase of relative level of exosomes in secreted EVs after overexpression nSMase2 in HEK293 cells.

The result proved the feasible and effective improvement of manufacturing exosomes by overexpressing nSMase2.

Figure 4. Characterization of secreted exosomes after overexpression of nSMase2 in HEK293 cells. (A and B) Representative screen shots of the NTA videos for EV from HEK293 cells under normal condition or after transfection with nSMase2 plasmid. (C-F) Size and intensity of EV from HEK293 cells under normal condition or after transfection with nSMase2 plasmid. (G) Concentration of different particle sizes of exosomes with (red line) or without (blue line) transfection with nSMase2 plasmid. The peak of size distribution of EV shifted from 170 nm to 120 nm after transfection with nsMase2 plasmid, indicating the increase in quantity of secreted exosomes.

NUDT_CHINA NJU_China

II.Collaboration with USTC-software(CLICK)

This year, we helped USTC-software by sending our part to them, which we submitted last year numbered for BBa_k1532006.

They asked us for help because they needed the plasmid luxR numbered for C0062 in their project, but what they obtained in the kit plate was proved to be wrong by sequencing. As luck would have it, we submitted it as one of our bricks last year, so we sent it to them as soon as possible to ensure their project runs smoothly.

You can goto USTC-software’s Wiki to affirm our contributions.

NUDT_CHINA USTC-software

III.Collaboration with NEFU_China(CLICK)

Wet Lab: Sent a plasmid vector to NEFU_China

This year, we helped NEFU_China by sending a plasmid vector to them.

We got in touch with NEFU_China in the 2015 CiCC (China iGEMer Committee). When we exchanged ideas with others, they expressed the difficulty they faced at that time that they were lack of a pivotal plasmid vector which is too hard to buy. We were pleased to offer it to them as we have stored it in our plasmid library. We can obtain a huge sense of satisfaction by helping our friends get over the trouble.

They asked us for help because they needed the plasmid luxR numbered for C0062 in their project, but what they obtained in the kit plate was proved to be wrong by sequencing. As luck would have it, we submitted it as one of our bricks last year, so we sent it to them as soon as possible to ensure their project runs smoothly.

You can goto NEFU_China’s Wiki to affirm our contributions.

We used and improved NEFU_China’s “Flight iGEM”

This year, we helped NEFU_China to improved their “Flight iGEM” platform which aims to build a friendly website to make the wiki development easier.

We got touch with NEFU_China in the 2015 CiCC (China iGEMer Committee). Then, we participated in the “Flight iGEM” beta test. Below is our contributions to “Flight iGEM”:

1. Improve the Human-Computer Interaction (HCI) for the “Flight iGEM” Magic function.

2. Figure out the BUG in the login bar which is significant because the 2015 wiki new rules forbad the revise of login bar.

3. Contact with the igem.org to make sure the feasibility of cleariGEM template.

We are the users of "Flight iGEM"

You can goto NEFU_China’s Software page to affirm our contributions and more details about the “Flight iGEM”.

NUDT_CHINA NEFU_China