Difference between revisions of "Team:Chalmers-Gothenburg/Protocols"

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<h1>Protocols</h1>
 
<h1>Protocols</h1>
  
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<p><b>PCR:</b></p><html><a href ="https://static.igem.org/mediawiki/2015/b/be/ChalmersGothenburgPCRprotocol.pdf"> PCR </a> </html>
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<html><a href ="https://static.igem.org/mediawiki/2015/b/be/ChalmersGothenburgPCRprotocol.pdf"> PCR </a> </html>
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<html><a href ="https://static.igem.org/mediawiki/2015/0/07/ChalmersGothenburgFastDigestRestrictionEnzymeProtocol.pdf"> FastDigest Restriction </a> </html>
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<html><a href ="https://static.igem.org/mediawiki/2015/c/c4/ChalmersGothenburgYeastTransformationByElectroporationProtocol.pdf"> Yeast Transformation by Electroporation </a> </html>
  
 
<p><b>PCR Purification:</b></p>
 
<p><b>PCR Purification:</b></p>

Revision as of 20:18, 18 September 2015



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Protocols

PCR

FastDigest Restriction

Yeast Transformation by Electroporation

PCR Purification:

Gel extraction:

Gibson Assembly Method:

Sticky-end DNA Insert Ligation into Vector DNA:

FastDigest Restriction:

Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation:

Yeast Genomic DNA Extraction LiOAc-SDS:

Yeast Transformation by Electroporation:

Test of Detection System in Saccharomyces cerevisiae:


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