Competent E. coli were transformed with 10 µL ligation product and plated on LB plate with ampicillin (BBa_K823023). No white colonies visible. Transformation failed.
Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Psalt tasA Transformation zp70"
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Latest revision as of 20:44, 18 September 2015
Psalt tasA Transformation (zp70)
Transformation was done to introduce the ligated plasmid DNA into E. coli.
To create a salt inducible promotor.
Transformation failed.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>
00:00, 26 Augsut 2015 - 00:00, 26 August 2015