Difference between revisions of "Team:Exeter/Results"

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<h1>Experiments &amp; Protocols</h1>
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<h1>Results</h1>
  
 
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<h4>Experimental validation of GreenFET1J (<a href="https://2015.igem.org/Team:Exeter/Parts#toeholds">BBa_K1586000</a>):</h4>
 
<h4>Experimental validation of GreenFET1J (<a href="https://2015.igem.org/Team:Exeter/Parts#toeholds">BBa_K1586000</a>):</h4>
  
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As was explained on the <a href="">experiments page</a>, GreenJ's (BBa_K1586000) function was characterised and validated by measuring fluorescence intensity of GFP in response to increasing amounts of trigger RNA.
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As has been stated previously, we wish our diagnostic test to be carried out in the field with little technical equipment/training, therefore the visualisation of the result must be simple. In order to do this, we decided that a colour change would be best, and that the expression of chromoproteins on activation of the toehold would be a good way to achieve this. In order to better inform ourselves and the model, and also to generate data which would be useful for testing toeholds with chromoproteins as the protein coding regions, we decided to further characterise some of the chromoproteins in the iGEM registry; <a href="http://parts.igem.org/Part:BBa_K1431814">amajLime</a>, <a href="http://parts.igem.org/Part:BBa_K1073020">aeBlue</a>, and <a href="http://parts.igem.org/Part:BBa_K1073022">eforRed</a>.
 
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<h4>Absorbance spectra:</h4>
 
<h4>Absorbance spectra:</h4>

Revision as of 20:49, 18 September 2015

Results

Toehold experiments

In silico testing:

Experimental validation of GreenFET1J (BBa_K1586000):

As was explained on the experiments page, GreenJ's (BBa_K1586000) function was characterised and validated by measuring fluorescence intensity of GFP in response to increasing amounts of trigger RNA.

Further characterisation:

Absorbance spectra:

Standard curve - concentration vs. OD:

  • Contact us:
    exeterigem@gmail.com