Difference between revisions of "Team:Dundee/Safety"

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<h2>Safety in iGEM</h2>
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
  
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    <header id="header-fingerprint">
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            <h1><highlight class="highlight">Fingerprint Ageing</highlight></h1>
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            <h3><highlight class="highlight">The Report</highlight></h3>
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<h4>Safe Project Design</h4>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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        <h1>Abstract</h1>
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            <p>Lanosterol synthase (LSS) an oxidosqualene cyclase (OSC) enzyme that specifically binds to squalene epoxide (2,3- oxidosqualene), which is present in fingerprints. We successfully managed to clone lanosterol synthase into pSB1C3 and the over expression vector pQE80-L and successfully characterised using an anti-His antibody. We then continued on in the hope to purify this enzyme from E. coli using Nickel affinity chromatography.</p>
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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<h4>Safe Lab Work</h4>
 
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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          <h1>Introduction</h1>
  
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<h4>Safe Shipment</h4>
 
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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        <h1>Future Work</h1>
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              <figure align="center">
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                <img class="report-img" src="https://static.igem.org/mediawiki/2015/8/8d/Finger-fig7.png">
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                    <figcaption class="report-img">
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                      <p><b>Figure 7 -</b>Crystal structure of lanosterol synthase with the active sites at positions 232 and 455 (red). The yellow arrows indicate the active sites in laonsterol synthase that will have to be mutated to inactivate the enzyme to avoid unwanted activity.</p>
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                    </figcaption>
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              </figure>
  
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                <a href="https://2015.igem.org/Team:Dundee/Lab_Book/Chromate_Detector" class="btn btn-primary btn-lg pull-right" role="button">Our Lab Records for Fingerprint Ageing</a>
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Revision as of 20:51, 18 September 2015

Fingerprint Ageing

The Report

Abstract

Lanosterol synthase (LSS) an oxidosqualene cyclase (OSC) enzyme that specifically binds to squalene epoxide (2,3- oxidosqualene), which is present in fingerprints. We successfully managed to clone lanosterol synthase into pSB1C3 and the over expression vector pQE80-L and successfully characterised using an anti-His antibody. We then continued on in the hope to purify this enzyme from E. coli using Nickel affinity chromatography.

Introduction

Future Work

Figure 7 -Crystal structure of lanosterol synthase with the active sites at positions 232 and 455 (red). The yellow arrows indicate the active sites in laonsterol synthase that will have to be mutated to inactivate the enzyme to avoid unwanted activity.

Our Lab Records for Fingerprint Ageing