Difference between revisions of "Team:Dundee/Safety"

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             <h1><highlight class="highlight">Fingerprint Ageing</highlight></h1>
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             <h1><highlight class="highlight">Safety</highlight></h1>
             <h3><highlight class="highlight">The Report</highlight></h3>
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         <h1>Abstract</h1>
 
         <h1>Abstract</h1>
            <p>Lanosterol synthase (LSS) an oxidosqualene cyclase (OSC) enzyme that specifically binds to squalene epoxide (2,3- oxidosqualene), which is present in fingerprints. We successfully managed to clone lanosterol synthase into pSB1C3 and the over expression vector pQE80-L and successfully characterised using an anti-His antibody. We then continued on in the hope to purify this enzyme from E. coli using Nickel affinity chromatography.</p>
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         <h1>Future Work</h1>
 
         <h1>Future Work</h1>
         
 
 
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                <img class="report-img" src="https://static.igem.org/mediawiki/2015/8/8d/Finger-fig7.png">
 
                    <figcaption class="report-img">
 
                      <p><b>Figure 7 -</b>Crystal structure of lanosterol synthase with the active sites at positions 232 and 455 (red). The yellow arrows indicate the active sites in laonsterol synthase that will have to be mutated to inactivate the enzyme to avoid unwanted activity.</p>
 
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                <a href="https://2015.igem.org/Team:Dundee/Lab_Book/Chromate_Detector" class="btn btn-primary btn-lg pull-right" role="button">Our Lab Records for Fingerprint Ageing</a>
 
  
  

Revision as of 20:52, 18 September 2015

Safety

Abstract

Introduction

Future Work