Difference between revisions of "Team:Aix-Marseille/Parts"

Line 324: Line 324:
 
                 <div class="col-md-12 text-center">
 
                 <div class="col-md-12 text-center">
 
                     <h2 class="gray">SPONSORS</h2>
 
                     <h2 class="gray">SPONSORS</h2>
−
                    <ul class="list-inline space30 icon">
+
<ul class="list-inline space30 icon">
−
                        <li><a href="http://polytech.univ-amu.fr/"><img src="http://i.imgur.com/iLFZ36R.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
  <li><a href="https://www.corning.com/worldwide/en/products/life-sciences.html">
−
                        <li><a href="http://www.crous-aix-marseille.fr/social/fsdie-social"><img src="http://i.imgur.com/KlP5G8h.png" class="img-grey mautomargin" width="200" height="150"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/8/87/Corninglogo.jpg" width="200" align="center"
−
                        <li><a href="http://www.univ-amu.fr/"><img src="http://i.imgur.com/EcfF9Kw.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    class="img-grey mautomargin"></a></li>
−
                        <li><a href="http://www.cnrs.fr/"><img src="http://i.imgur.com/IaWMSIr.jpg" width="200" height="150" class="img-grey mautomargin"></a></li>
+
  <li><a href="http://www.crous-aix-marseille.fr/social/fsdie-social">
−
                        <li><a href="http://www3.gehealthcare.fr/"><img src="http://i.imgur.com/ryVz5vv.jpg" width="200" height="100" class="img-grey mautomargin"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/1/18/KlP5G8h.jpg"  
−
                        <li><a href="https://www.corning.com/worldwide/en/products/life-sciences.html"><img src="http://i.imgur.com/ycgjpR7.jpg" width="225" height="100" class="img-grey mautomargin"></a></li>
+
    class="img-grey mautomargin" width="200" align="center"></a></li>
−
                        <li><a href="http://www.grandluminy.com/"><img src="http://i.imgur.com/GK5IMdz.jpg" width="200" height="100" class="img-grey mautomargin"></a></li>
+
  <li><a href="http://www.cnrs.fr/">
−
                        <li><a href="https://www.eppendorf.com/FR-fr/"><img src="http://i.imgur.com/jUjj2YD.jpg" width="250" height="100" class="img-grey mautomargin"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/8/8e/CNRSlogo.jpg"
−
                        <li><a href="http://www.dutscher.com/frontoffice/home;jsessionid=4164FFDE7B048361D89406E266542A13"><img src="http://i.imgur.com/wjZ6U1A.jpg" width="200" height="100" class="img-grey mautomargin"></a></li>
+
    width="200" class="img-grey mautomargin" align="center"></a></li>
−
<li><a href="http://www.mn-net.com/"><img src="http://i.imgur.com/apuJ5in.jpg" width="200" height="100" class="img-grey mautomargin"></a></li>
+
  <li><a href="https://www.qiagen.com/fr/">
−
<li><a href="https://fr.groupeonet.com/Onet-Proprete-et-Services"><img src="http://i.imgur.com/5tokNzS.png" width="200" height="100" class="img-grey mautomargin"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/f/f1/Qialogo.jpg" width="200" align="center"
−
<li><a href="https://www.qiagen.com/fr/"><img src="http://i.imgur.com/4jXkcLB.jpg" width="200" height="100"class="img-grey mautomargin"></a></li>
+
    class="img-grey mautomargin"></a></li>
−
<li><a href="https://www.sigmaaldrich.com/france.html"><img src="http://i.imgur.com/PUNXaNj.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
  <li><a href="http://www.univ-amu.fr/">
−
<li><a href="http://www.starlab.de/int/?l=5"><img src="http://i.imgur.com/SsgB8IC.jpg" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/3/38/EcfFf9Kw.png"  
−
<li><a href="http://fr.ambafrance-us.org/"><img src="http://i.imgur.com/m3ieC2R.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    class="img-grey mautomargin" width="200" align="center" ></a></li>
−
<li><a href="https://www.neb.com/"><img src="http://i.imgur.com/ll3uagr.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
  <li><a href="http://polytech.univ-amu.fr/">
−
<li><a href="http://sciences.univ-amu.fr/"><img src="http://i.imgur.com/FtFgEFj.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/1/10/ILFZ36R.png"  
−
<li><a href="https://eu.idtdna.com/site"><img src="http://i.imgur.com/ocgTw7q.jpg" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    class="img-grey mautomargin" width="200" align="center"></a></li>
−
 
+
  <li><a href="http://www.grandluminy.com/">
−
 
+
    <img src="https://static.igem.org/mediawiki/2015/6/66/GandLlogo.jpg" width="200" align="center"
−
 
+
    class="img-grey mautomargin"></a></li>
−
                    </ul>
+
  <li><a href="https://www.eppendorf.com/FR-fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/d/db/Eppendorflogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.dutscher.com/frontoffice/home">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/10/Deutcherflogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.mn-net.com/">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/1b/MNlogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="https://fr.groupeonet.com/Onet-Proprete-et-Services">
 +
    <img src="https://static.igem.org/mediawiki/2015/3/3d/5tokNzS.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www3.gehealthcare.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/b/bd/GEHlogo.jpg" width="200"  
 +
    class="img-grey mautomargin" align="center" ></a></li>
 +
  <li><a href="https://www.sigmaaldrich.com/france.html">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/41/PUNXaNj.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="http://www.starlab.de/int/?l=5">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/13/StarLablogo.jpg" align="center"
 +
    class="img-grey mautomargin" width="200" height="100"></a></li>
 +
  <li><a href="http://fr.ambafrance-us.org/">
 +
    <img src="https://static.igem.org/mediawiki/2015/2/2b/M3ieC2R.jpg" align="center"
 +
    class="img-grey mautomargin" width="200" height="100"></a></li>
 +
  <li><a href="https://www.neb.com/">
 +
    <img src="https://static.igem.org/mediawiki/2015/a/a9/Ll3uagr.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="http://sciences.univ-amu.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/4a/FtFgEFj.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="https://eu.idtdna.com/site">
 +
    <img src="https://static.igem.org/mediawiki/2015/d/d9/IDTlogo.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
</ul>
  
 
                    
 
                    
Line 354: Line 387:
 
               <div class="space20"></div>
 
               <div class="space20"></div>
 
         </div>
 
         </div>
−
 
 
     </section>
 
     </section>
 
       <!-- start footer -->
 
       <!-- start footer -->

Revision as of 21:16, 18 September 2015

Chew fight

Parts

New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:

  • -T7 promoter with RBS (Bba_K525998) and optimized Laccase from T.Thermophilus (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)

  • -T7 promoter with RBS and optimized Laccase from T.Thermophilus, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)










New biobricks we designed

We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:
  • -Cytochrome C Synechocystis sp (BBa_K1601001), a cyanobacteria that suggests a cytochrome c

  • -Cytochrome C of S.oneidensis (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.

  • -Heme A from E.coli K12 (BBa_K1601002)that enables the heme overproduction

  • -Yeast signal CXXCH from Synechocystis sp responsible for the S.oneidensis CytC translocation into the periplasm using TAT system (BBa_S05319)

  • -Yeast signal CXXCH from Synechocystis sp responsible for the Synechocystis sp CytC translocation into the periplasm using TAT system (BBa_S05320)







Linkers we designed

We designed some linker to facilitate the meeting of the laccase and the cytochrom C

  • Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC S.oneidensis (BBa_K1601008)

  • Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC P.denitrificans (BBa_K1601011)

  • Rigid and structured linker designed to link (or connect) T.Thermophilus and CytC Synechocystis sp(BBa_K1601013)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC P.denitrificans (BBa_K1601009)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC Synechocystis sp (BBa_K1601010)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC S.oneidensis(BBa_K1601012)







New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:
  • -Yeast signal CXXCH from Synechocystis sp responsible for the S. oneidensis CytC translocation into the periplasm using TAT system
  • -T7 promoter with RBS and Laccase E.coli (Bba_K863006) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601003)
  • -T7 promoter with RBS and Laccase T.Thermophilus (Bba_K863011) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601004)
  • -Cytochrome C Heme Lyase (CCHL)(BBa_K1601005)
  • -Yeast signal CXXCH (BBa_K1601006)
  • -Cytochrome C P.Denitrificans (BBa_K1601007)
  • -Cytochrome C Synechocystis sp - His Tag (BBa_S05314)
  • -Cytochrome C S.oneidensis - HisTag (BBa_S05315)






New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:
  • Yeast signal CXXCH - Cytochrome C Synechocystis sp (BBa_S05316)
  • Cytochrome C P.Denitrificans - His Tag (BBa_S05317)
  • Yeast signal CXXCH - Cytochrome C S.oneidensiswithout stop codon (BBa_S05318)
  • T7 promoteur,RBS - Cytochrome C S.oneidensis - HisTag (BBa_K1601016)






MORE INFORMATION ON FACEBOOK !

SPONSORS

Useful Links

Contact Us


  • Aix Marseille Université
  • Marseille
  • France

Chew figth project, for the iGEM competition. See you soon in Boston !