Difference between revisions of "Team:UI Indonesia/project"

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function myFunction2() {

document.getElementById("judul").innerHTML = "Grand Design";

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document.getElementById("isi").innerHTML = "c";

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document.getElementById("isi").innerHTML = "The Grand Design – Toggle switch with spermicidal peptide. We design a device for gram positive bacteria. After discussion, we chose to use B. subtilis 168and E. coli BL21as our host. B. subtilis 168 is a model for gram positive toggle switch. E. coli BL21 and B. subtilis 168 are chosen for chassis for expression of SboA.

+

For grand design we designeda toggle switch with the spermicidal protein; Subtilosin A (SboA) in the system. In the other side of the switch, we designed a suicide protein: NdoA. The promoter in the system are Phyperspank(Phs) and PxylA. Phyperspank is a constitutive promoter for gram positive when the repressor protein, LacI is not present. When present, LacI bound to Phs, inhibiting the expression of the promoter. However, with addition of lactose or IPTG, LacI would bind to IPTG and detach from the promoter, causing the promoter to express the protein downstream. PxylA is another constitutive promoter for gram positive. PxylA is repressed by xylR. Xylose would bind to xylR and cause PxylA to express the protein downstream. We express xylR under the effect of Phs and express LacI under the effect of PxylA. Thus, when one promoter is active, it repress the other promoter. When PxylA is active, it express SboA, the spermicidal protein. It also express LacI which inhibit the other promoter, Phs. When induced with IPTG, LacI binds to IPTG and inhibition on Phs decrease. Thus protein downstream of Phs active, which is XylR. XylR repress PxylA, thus the expression of SboA stop, and the production of NdoA started. This Grand Design was the ideal design. However, we are not testing this device as a whole due to safety reason. The safety reason issue is the pathogenicity of the bacteria if we test this device as a whole.";

}

@@ Line 246: / Line 246: @@
 function myFunction2() {
 document.getElementById("judul").innerHTML = "Grand Design";
-     document.getElementById("isi").innerHTML = "c";
+     document.getElementById("isi").innerHTML = "The Grand Design – Toggle switch with spermicidal peptide.<br><br><br>We design a device for gram positive bacteria. After discussion, we chose to use B. subtilis 168and E. coli BL21as our host. B. subtilis 168 is a model for gram positive toggle switch. E. coli BL21 and B. subtilis 168 are chosen for chassis for expression of SboA.
+For grand design we designeda toggle switch with the spermicidal protein; Subtilosin A (SboA) in the system. In the other side of the switch, we designed a suicide protein: NdoA.<br>The promoter in the system are Phyperspank(Phs) and PxylA. Phyperspank is a constitutive promoter  for gram positive when the repressor protein, LacI is not present. When present, LacI bound to Phs, inhibiting the expression of the promoter. However, with addition of lactose or IPTG, LacI would bind to IPTG and detach from the promoter, causing the promoter to express the protein downstream.<br>PxylA is another constitutive promoter for gram positive. PxylA is repressed by xylR. Xylose would bind to xylR and cause PxylA to express the protein downstream.<br>We express xylR under the effect of Phs and express LacI under the effect of PxylA. Thus, when one promoter is active, it repress the other promoter. When PxylA is active, it express SboA, the spermicidal protein. It also express LacI which inhibit the other promoter, Phs. When induced with IPTG, LacI binds to IPTG and inhibition on Phs decrease. Thus protein downstream of Phs active, which is XylR. XylR repress PxylA, thus the expression of SboA stop, and the production of NdoA started.<br>This Grand Design was the ideal design. However, we are not testing this device as a whole due to safety reason. The safety reason issue is the pathogenicity of the bacteria if we test this device as a whole.";
 }