Difference between revisions of "Team:Kent/Notebook"

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     <th> 15 </th><th> 16 </th><th> 17 </th><th> 18 </th><th> 19 </th><th> 20 </th><th> 21 </th>
 
     <th> 15 </th><th> 16 </th><th> 17 </th><th> 18 </th><th> 19 </th><th> 20 </th><th> 21 </th>
 
<tr>
 
<tr>
−
     <th> <a href="#day1">22</a> </th><th><a href="#day2"> 23</a> </th><th><a href="#day3"> 24</a> </th><th><a href="#day4"> 25</a> </th><th><a href="#day5"> 26</a> </th><th> 27 </th><th> 28 </th>
+
     <th> 22 </th><th><a href="#MEETING 1"> 23</a> </th><th> 24</th><th> 25 </th><th>26</th><th> 27 </th><th> 28 </th>
 
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<tr>
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     <th><a href="#day6"> 29</a> </th><th> <a href="#day7"> 30 </a> </th><th> </th><th> </th><th> </th><th> </th><th> </th>
+
     <th>29</th><th> <a href="#MEETING 2"> 30 </a> </th><th> </th><th> </th><th> </th><th> </th><th> </th>
 
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     <th> M </th> <th> T </th> <th> W </th><th> T </th><th> F </th><th> S </th><th> S </th>
 
     <th> M </th> <th> T </th> <th> W </th><th> T </th><th> F </th><th> S </th><th> S </th>
 
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     <th>  </th><th>  </th><th> <a href="#day8"> 1 </a> </th><th> <a href="#day9"> 2 </a> </th><th> <a href="#day10"> 3 </a> </th><th> 4 </th><th> 5 </th>
+
     <th>  </th><th>  </th><th> 1 </th><th> 2 </th><th> 3 </th><th> 4 </th><th> 5 </th>
 
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<tr>
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     <th> 6</th><th> 7 </th><th> 8 </th><th> 9 </th><th> 10 </th><th> 11 </th><th> 12 </th>
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     <th> 6</th><th> <a href="#MEETING 3"> 7</a> </th><th> 8 </th><th> 9 </th><th> 10 </th><th> 11 </th><th> 12 </th>
 
<tr>
 
<tr>
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     <th> 13 </th><th> 14 </th><th> 15 </th><th> 16 </th><th> 17 </th><th> 18 </th><th> 19 </th>
+
     <th> 13 </th><th> <a href="#MEETING 4"> 14 </a>  </th><th> 15 </th><th> 16 </th><th> 17 </th><th> 18 </th><th> 19 </th>
 
<tr>
 
<tr>
−
     <th> 20 </th><th> 21 </th><th> 22 </th><th> 23 </th><th> 24 </th><th> 25 </th><th> 26 </th>
+
     <th> 20 </th><th> <a href="#MEETING 5"> 21 </a> </th><th> 22 </th><th> 23 </th><th> 24 </th><th> 25 </th><th> 26 </th>
 
<tr>
 
<tr>
−
     <th> 27 </th><th> 28 </th><th> 29</th><th>30 </th><th> 31 </th><th> </th><th> </th>
+
     <th> 27 </th><th> <a href="#MEETING 6"> 28 </a>  </th><th> 29</th><th>30 </th><th> 31 </th><th> </th><th> </th>
 
<tr>
 
<tr>
 
</table>
 
</table>
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     <th>  </th><th>  </th><th>  </th><th>  </th><th>  </th><th> 1 </th><th> 2 </th>
 
     <th>  </th><th>  </th><th>  </th><th>  </th><th>  </th><th> 1 </th><th> 2 </th>
 
<tr>
 
<tr>
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     <th> 3</th><th> 4 </th><th> 5 </th><th> 6 </th><th> 7 </th><th> 8 </th><th> 9 </th>
+
     <th> 3</th><th><a href="#MEETING 7"> 4 </a>  </th><th> 5 </th><th> 6 </th><th> 7 </th><th> 8 </th><th> 9 </th>
 
<tr>
 
<tr>
−
     <th> 10 </th><th> 11 </th><th> 12 </th><th> 13 </th><th> 14 </th><th> 15 </th><th> 16 </th>
+
     <th> 10 </th><th> <a href="#MEETING 8"> 11 </a>  </th><th> 12 </th><th> 13 </th><th> 14 </th><th> 15 </th><th> 16 </th>
 
<tr>
 
<tr>
−
     <th> 17 </th><th> 18 </th><th> 19 </th><th> 20 </th><th> 21 </th><th> 22 </th><th> 23 </th>
+
     <th> 17 </th><th> <a href="#MEETING 9"> 18 </a> </th><th> 19 </th><th> 20 </th><th> 21 </th><th> 22 </th><th> 23 </th>
 
<tr>
 
<tr>
−
     <th> 24 </th><th> 25 </th><th> 26</th><th>27 </th><th> 28 </th><th> 29 </th><th> 30 </th>
+
     <th> 24 </th><th><a href="#MEETING 10"> 25 </a>  </th><th> 26</th><th>27 </th><th> 28 </th><th> 29 </th><th> 30 </th>
 
<tr>
 
<tr>
 
     <th> 31</th><th>  </th><th> </th><th></th><th> </th><th> </th><th> </th>
 
     <th> 31</th><th>  </th><th> </th><th></th><th> </th><th> </th><th> </th>
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     <th> M </th> <th> T </th> <th> W </th><th> T </th><th> F </th><th> S </th><th> S </th>
 
     <th> M </th> <th> T </th> <th> W </th><th> T </th><th> F </th><th> S </th><th> S </th>
 
<tr>
 
<tr>
−
     <th>  </th><th> 1 </th><th> 2 </th><th>3  </th><th> 4 </th><th> 5 </th><th> 6 </th>
+
     <th>  </th><th> <a href="#MEETING 11"> 1 </a>  </th><th> 2 </th><th>3  </th><th> 4 </th><th> 5 </th><th> 6 </th>
 
<tr>
 
<tr>
−
     <th> 7</th><th> 8 </th><th>9 </th><th> 10 </th><th> 11 </th><th> 12 </th><th> 13 </th>
+
     <th> 7</th><th> <a href="#MEETING 12"> 8 </a>  </th><th>9 </th><th> 10 </th><th> 11 </th><th> 12 </th><th> 13 </th>
 
<tr>
 
<tr>
−
     <th> 14 </th><th> 15 </th><th> 16 </th><th> 17 </th><th> 18 </th><th> 19 </th><th> 20 </th>
+
     <th> 14 </th><th> <a href="#MEETING 13"> 15 </a>  </th><th> 16 </th><th> 17 </th><th> 18 </th><th> 19 </th><th> 20 </th>
 
<tr>
 
<tr>
−
     <th> 21 </th><th> 22 </th><th> 23 </th><th> 24 </th><th> 25 </th><th> 26 </th><th> 27 </th>
+
     <th> 21 </th><th> <a href="#MEETING 14"> 22 </a>  </th><th> 23 </th><th> 24 </th><th> 25 </th><th> 26 </th><th> 27 </th>
 
<tr>
 
<tr>
 
     <th> 28 </th><th> 29 </th><th> 30</th><th> </th><th> </th><th> </th><th> </th>
 
     <th> 28 </th><th> 29 </th><th> 30</th><th> </th><th> </th><th> </th><th> </th>
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<div style="margin:2.5%">
 
<div style="margin:2.5%">
−
<a name="day1"></a><h4> Day 1 Wet lab <i>22/06/15</i> </h4>
+
<a name="MEETING 1"></a><h4> MEETING 1 <i>23/06/15</i> </h4>
−
<p><li> Made LB plates </li>
+
<p>
−
      <li> Made LB broth </li>
+
−
      <li> TIPS autoclaved </li>
+
 
</p>
 
</p>
  
−
<a name="day2"></a><h4> Day 2 Wet lab <i>23/06/15</i> </h4>
+
<a name="MEETING 2"></a><h4> MEETING 2 <i>30/06/15</i> </h4>
 
<p><li> Set up Top10 overnight, VS45 overnight, US348 overnight </li>
 
<p><li> Set up Top10 overnight, VS45 overnight, US348 overnight </li>
 
       <li> Meeting with advisors to discuss progress </li>
 
       <li> Meeting with advisors to discuss progress </li>
 
</p>
 
</p>
  
−
<a name="day3"></a><h4> Day 3 Wet lab <i>24/06/15</i></h4>
+
<a name="MEETING 3"></a><h4> MEETING 3 <i>7/07/15</i></h4>
−
<p><li> Filter sterilise the buffer</li>
+
<p>
−
      <li> 250ml no antibiotic broth, put top10 cells in</li>
+
−
      <li> Incubate until optical density is 0.6 </li>
+
−
      <li> Miniprep PSB1C3 plasmid </li>  
+
 
</p>
 
</p>
  
  
−
<a name="day4"></a><h4> Day 4 Wet lab <i> 25/06/15 </i> </h4>
+
<a name="MEETING 4"></a><h4> MEETING 4 <i> 14/07/15 </i> </h4>
−
<p> <li> Transformation </li>
+
<p>  
−
      <li> Prepare SBC media </li>
+
−
      <li> Digest of PSB1c3 from MS348 </li>
+
 
</p>
 
</p>
  
−
<a name="day5"></a><h4> Day 5 Wet lab <i> 26/06/15 </i> </h4>
+
<a name="MEETING 5"></a><h4> MEETING 5 <i> 21/07/15 </i> </h4>
−
<p><li> Calculated competent cell efficiency </li>
+
<p>
−
      <li> Agarose gel formation </li>
+
−
      <li> Gel electrophoresis </li>
+
 
</p>
 
</p>
  
−
<a name="day6"></a><h4> Day 6 Wet lab <i> 29/06/15 </i> </h4>
+
<a name="MEETING 6"></a><h4> MEETING 6<i> 28/07/15 </i> </h4>
−
<p></li> Transforming linear PSB1C3 </li>
+
<p>
 
</p>
 
</p>
  
−
<a name="day7"></a><h4> Day 7 Wet lab <i> 30/06/15 </i> </h4>
+
<a name="MEETING 7"></a><h4> MEETING 7 <i> 04/08/15 </i> </h4>
−
<p><li> Miniprep of PSB1A3 plasmid </li>
+
<p>
−
      <li> Gel electrophoresis of a large quantity of PSB1C3 </li>
+
−
      <li> Meeting with advisors to discuss progress </li>
+
 
</p>
 
</p>
  
−
<div class="notesideimage">
+
<a name="MEETING 8"></a><h4> MEETING 8 <i> 11/08/15 </i> </h4>
−
    <img src="https://static.igem.org/mediawiki/2015/f/ff/Team_Kent_CompetentCells.jpg" style="float: right; width: 50%; margin-right: 1%;  class="sideimage"/>
+
<p>  
−
</div>
+
</p>
  
−
<a name="day8"></a><h4> Day 8 Wet lab <i> 01/07/15 </i> </h4>
+
<a name="MEETING 9"></a><h4> MEETING 9 <i> 18/08/15 </i> </h4>
−
<p> <li> Gel electrophoresis of the purified DNA extracted </li>
+
<p>  
−
<li> Transformation of PSB1A3 circular plasmid to VS45 cells (competent) </li>
+
</p>
−
<li> Digest of all PSB1c3 plasmids </li> </p>
+
  
−
<a name="day9"></a><h4> Day 9 Wet lab <i> 02/07/15 </i> </h4>
+
<a name="MEETING 10"></a><h4>MEETING 10 <i> 25/08/15 </i> </h4>
−
<p> <li> Competent cells transformation efficiency </li>
+
<p>  
−
<li> Mini-prep of 1MS340 to get PSB1c3 plasmid </li>
+
</p>
−
<li> Gel extraction of big kb digest </li>
+
−
<li> Quantification of the digest. Added sample buffer to each digest </li>  </p>
+
  
−
<a name="day10"></a><h4> Day 10 Wet lab <i> 03/07/15 </i> </h4>
+
<a name="MEETING 11"></a><h4> MEETING 11 <i> 01/09/15 </i> </h4>
−
<p> <li> Transformation efficiency </li>
+
<p>  
 
</p>
 
</p>
  
 +
<a name="MEETING 12"></a><h4> MEETING 12 <i> 08/09/15 </i> </h4>
 +
<p>
 +
</p>
  
 +
<a name="MEETING 13"></a><h4>MEETING 13<i> 15/09/15 </i> </h4>
 +
<p>
 +
</p>
 +
 +
<a name="MEETING 14"></a><h4> MEETING 14<i> 22/09/15 </i> </h4>
 +
<p>
 +
</p>
  
 
</div>
 
</div>

Revision as of 16:36, 7 July 2015


iGEM Kent 2015

Notebook

June

M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30

July

M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 2930 31

August

M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 2627 28 29 30
31

September

M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30






















MEETING 1 23/06/15

MEETING 2 30/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight
  • Meeting with advisors to discuss progress

    • MEETING 3 7/07/15

    MEETING 4 14/07/15

    MEETING 5 21/07/15

    MEETING 6 28/07/15

    MEETING 7 04/08/15

    MEETING 8 11/08/15

    MEETING 9 18/08/15

    MEETING 10 25/08/15

    MEETING 11 01/09/15

    MEETING 12 08/09/15

    MEETING 13 15/09/15

    MEETING 14 22/09/15